14 research outputs found
Joint association of early life lower respiratory tract infection and <i>PAI-1</i> risk genotype (<i>rs2227631</i>) with Asthma.
<p>LRI indicates subjects who were diagnosed with a lower respiratory tract infection requiring medical attention before age 2 years old. Adjusted for age, sex, ethnicity, ancestry, socioeconomic factors, environmental factors (history of farm animal exposure and received antibiotics during first year), family history of asthma and recruitment site.</p
Adjusted effect of <i>rs2227631</i>, infection and <i>rs2227631</i> gene-infection interaction on asthma exacerbations.
<p>Adjusted effect of <i>rs2227631</i>, infection and <i>rs2227631</i> gene-infection interaction on asthma exacerbations.</p
Demographic and clinical characteristics of subjects.
<p>Demographic and clinical characteristics of subjects.</p
Adjusted effect of <i>rs2227631</i>, infection and <i>rs2227631</i>gene-infection interaction on lung function in asthmatic subjects.
<p>Adjusted effect of <i>rs2227631</i>, infection and <i>rs2227631</i>gene-infection interaction on lung function in asthmatic subjects.</p
Joint association of bronchiolitis/ RSV and <i>PAI-1</i> risk genotype (<i>rs2227631</i>) with Asthma.
<p>RSV indicates subjects who were diagnosed with bronchiolitis or RSV before age 2 years old. Adjusted for age, sex, ethnicity, ancestry, socioeconomic factors, environmental factors (history of farm animal exposure and received antibiotics during first year), family history of asthma and recruitment site.</p
Adjusted effects of <i>rs2227631</i>, and infection on asthma.
<p>Adjusted effects of <i>rs2227631</i>, and infection on asthma.</p
Association results of 593 CNVs to asthma.
<p>CNVs for association testing in the full Hutterite pedigree were identified in the 16 sequenced genomes. The points for these CNVs are colored based on the results of the whole-genome sequencing to represent whether the variant was observed in cases only (red), control individuals only (blue), or in both case and control individuals (gray). The genomic position is represented on the x-axis and the −log<sub>10</sub>(p-value) of the nominal association of each CNV to asthma in the full Hutterite pedigree is on the y-axis.</p
Overview of sequenced genomes.
<p>Ideograms of the autosomes and X chromosome are shown. Red bars represent segments of autozygosity observed in five individuals with asthma (parents of the sequenced trios) and yellow triangles represent asthma-specific gene-disruptive SNVs or indels. The regions of autozygosity in multiple cases on chromosomes 1q and 5q were also observed to be autozygous in at least one control individual.</p
Comparison of CNV allele frequency between Hutterites and CEU.
<p>A histogram of the difference in allele frequency for the non-reference allele between Hutterites and the CEU individuals from the 1000 Genomes Project is shown. On the x-axis are bins for the absolute value of the non-reference allele frequency in the Hutterites minus the allele frequency in CEU. The y-axis represents the number of CNVs in each bin.</p
Whole-Genome Sequencing of Individuals from a Founder Population Identifies Candidate Genes for Asthma
<div><p>Asthma is a complex genetic disease caused by a combination of genetic and environmental risk factors. We sought to test classes of genetic variants largely missed by genome-wide association studies (GWAS), including copy number variants (CNVs) and low-frequency variants, by performing whole-genome sequencing (WGS) on 16 individuals from asthma-enriched and asthma-depleted families. The samples were obtained from an extended 13-generation Hutterite pedigree with reduced genetic heterogeneity due to a small founding gene pool and reduced environmental heterogeneity as a result of a communal lifestyle. We sequenced each individual to an average depth of 13-fold, generated a comprehensive catalog of genetic variants, and tested the most severe mutations for association with asthma. We identified and validated 1960 CNVs, 19 nonsense or splice-site single nucleotide variants (SNVs), and 18 insertions or deletions that were out of frame. As follow-up, we performed targeted sequencing of 16 genes in 837 cases and 540 controls of Puerto Rican ancestry and found that controls carry a significantly higher burden of mutations in <i>IL27RA</i> (2.0% of controls; 0.23% of cases; nominal p = 0.004; Bonferroni p = 0.21). We also genotyped 593 CNVs in 1199 Hutterite individuals. We identified a nominally significant association (p = 0.03; Odds ratio (OR) = 3.13) between a 6 kbp deletion in an intron of <i>NEDD4L</i> and increased risk of asthma. We genotyped this deletion in an additional 4787 non-Hutterite individuals (nominal p = 0.056; OR = 1.69). <i>NEDD4L</i> is expressed in bronchial epithelial cells, and conditional knockout of this gene in the lung in mice leads to severe inflammation and mucus accumulation. Our study represents one of the early instances of applying WGS to complex disease with a large environmental component and demonstrates how WGS can identify risk variants, including CNVs and low-frequency variants, largely untested in GWAS.</p></div