EBNA-1 quasispecies detected with FLX sequencing.

<p>Minority EBV sequence variants at a level of ≥10% were detected in two samples only. Sequence mixtures present at a ≥5% threshold revealed minority variants in 3.6% of investigated nucleotide positions (27/749 nucleotides sequenced,), increasing to 5.0% at a ≥2% cut-off (37/749 nucleotides,) and 8.3% at a ≥1% cut-off (62/749 nucleotides,). Samples 7, 8 and 13 did not have minority species present at ≥1%.</p

Putative HLA-DRB1*15 binders within autologous EBNA-1 peptide sequences and candidate myelin antigens.

<p>Putative HLA-DRB1*15 binders within autologous EBNA-1 peptide sequences and candidate myelin antigens.</p

EBNA-1 sequence variation identified with next-generation sequencing technology, and impact on HLA-DRB1*15 binding affinity within known epitopes.

<p>The two most frequent variants for the A) AEG epitope and B) MVF epitope are printed in bold, the most frequent variant is underlined. All epitopes are predicted to be weak binders (affinity 50nM-500nM).</p

Extended axoglial brain protein dataset with HLA-DRB1*1501 predicted brain epitopes overlapping with predicted EBV binders.

<p>Extended axoglial brain protein dataset with HLA-DRB1*1501 predicted brain epitopes overlapping with predicted EBV binders.</p

EBNA-1 sequence conservation and relationship to NetMHCII predicted HLA-DRB1*DR15-restricted epitopes derived from patient MS sequences.

<p>Shown are EBNA-1 amino acid positions 473–593. Predicted epitopes: blue boxes, known epitopes: black underlined, predicted epitope core: grey shade.</p

Age and gender distribution of patients recruited from the Perth Demyelinating Disease Database.

<p>Age and gender distribution of patients recruited from the Perth Demyelinating Disease Database.</p

Phylogenetic tree of C-terminal EBNA-1 Sanger sequences including the reference strains B95-8, AG876, GD1 and HKNPC1.

<p>Phylogenetic tree covering nucleotide positions B95-8: 109135–109815; EBNA-1: 1186–1866; blue triangles: EBV reference strains; red dots: MS cases (n = 53).</p