MA plots comparing the magnitude of change (log(signal array1) - log(signal array2)) on the y axis against the average log signal intensity (x axis)

<p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p> The green threshold lines show ± 2-fold changes. The color coding of the plot indicates the density of probesrepresented by that data point. The kits compared in each plot are given on the right hand side and the donor sample labeled by the kit is indicated at the top of each plot

Box plots of logged probe level signal intensities for each of the GeneChip arrays included in this study

<p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p

Overlaid electropherograms from the analysis of unfragmented biotinylated cRNA products from the IVT reactions of the 3 different labeling kits by the Agilent 2100 Bioanalyzer

<p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p> The replicate reactions from donor A are shown for each kit: One-Cycle data represented as blue and green line; BioArray as black and orange and Superscript by the pink and turquoise lines. 1 μl of the final volume (One-Cycle = 21 μl; BioArray = 60 μl; Superscript = 100 μl) of purified IVT reaction is loaded. The RNA ladder (peaks represented in red) contains a mixture of RNAs of known concentration and size (50 (lower marker) 200, 500, 1,000, 2,000, 4,000, and 6,000 bases from left to right)

Bar chart showing averaged signal intensities for all internal control probesets (QC2) with corresponding standard deviations

<p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p

Evaluation of transcripts spiked into the total RNA sample (poly-A controls, QC3)

<p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p> Averaged signal intensity of each of the 3 GeneChips showing the standard deviation

Overlaid Bioanalyzer electropherograms for fragmented labeled cRNA targets showing the size distribution of fragmented target

<p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p> One-Cycle replicates (blue and green); Superscript replicates (orange and black); BioArray replicates (pink and turquoise). RNA ladder in red showing the lower alignment marker and the 200 and 500 base markers