Linear regression and significance among Th1, Th2, Th17 and Treg profile targets based on ΔC_T values, total number of fungal cells, and number of viable fungal cells.

<p>Analysis of T cell subsets: Treg (A to E), Th1 (F and G), Th2 (H to L), Th17 (M to P). <b>#</b>: in front of the regression lines represents significant correlation (p and r² values). Regression analysis was performed using patient data.</p

Gene expression analysis of Treg profile targets.

<p>(A) Comparison of transcription factor FoxP3 between JLD patients and healthy controls. (B) Comparison of FoxP3 transcription factor between patients with a single lesion and patients with multiple lesions. (C) Comparison of CTLA4 marker expression between JLD patients and healthy controls. (D) Comparison of CTLA4 marker expression between patients with a single lesion and patients with multiple lesions. (E) Comparison of IL-10 cytokine expression between JLD patients and healthy controls. (F) Comparison of IL-10 cytokine expression between patients with a single lesion and patients with multiple lesions. (G) Comparison of TGF-β1 cytokine expression between JLD patients and healthy controls. (H) Comparison of TGF-x1 cytokine expression between patients with a single lesion and patients with multiple lesions. (I) Comparison of IKZF2 marker expression between JLD patients and healthy controls. (J) Comparison of IKZF2 marker expression between patients with a single lesion and patients with multiple lesions. Statistical analysis: Mann-Whitney U test (#p≤0.05)</p

Gene expression analysis of Th1 profile targets.

<p>(A) Comparison of T-bet transcription factor expression between JLD patients and healthy controls. (B) Comparison of T-bet transcription factor expression between patients with single lesion and multiple lesions. (C) Comparison of IFN-γ cytokine expression between JLD patients and healthy controls. (D) Comparison of IFN-γ cytokine expression between patients with a single lesion and patients with multiple lesions. Statistical analysis: Mann-Whitney U test (#p≤0.05)</p

Gene expression analysis of Th2 profile targets.

<p>(A) Comparison of the GATA3 transcription factor between JLD patients and healthy controls. (B) Comparison of GATA3 transcription factor between patients with a single lesion and patients with multiple lesions. (C) Comparison of IL-4 cytokine expression between JLD patients and healthy controls. (D) Comparison of IL-4 cytokine expression between patients with a single lesion and patients with multiple lesions. (E) Comparison of IL-5 cytokine between JLD patients and healthy controls. (F) Comparison of IL-5 cytokine expression between patients with a single lesion and patients with multiple lesions. (G) Comparison of IL-13 cytokine expression between JLD patients and healthy controls. (H) Comparison of IL-13 cytokine expression between patients with a single lesion and patients with multiple lesions. (I) Comparison of IL-33 cytokine expression between JLD patients and healthy controls. (J) Comparison of IL-33 cytokine expression between patients with a single lesion and patients with multiple lesions. Statistical analysis: Mann-Whitney U test (#p≤ 0.05)</p

Gene expression analysis of Th17 profile targets.

<p>(A) Comparison of RORC transcription factor between JLD patients and healthy controls. (B) Comparison of RORC transcription factor between patients with a single lesion and patients with multiple lesions. (C) Comparison of IL-17A cytokine expression between JLD patients and healthy controls. (D) Comparison of IL-17A cytokine expression between patients with a single lesion and patients with multiple lesions. (E) Comparison of IL-17F cytokine expression between JLD patients and healthy controls. (F) Comparison of IL-17F cytokine expression between patients with a single lesion and patients with multiple lesions. (G) Comparison of IL-22 cytokine expression between JLD patients and healthy controls. (H) Comparison of IL-22 cytokine expression between patients with single lesion and patients with multiple lesions. Statistical analysis: Mann-Whitney U test (#p≤0.05)</p