Multiple linear regression.

Predictors for relative CSF phosphofetuin-A/relative serum phosphofetuin-A quotient. (PDF)</p

Concentrations of fetuin-A in serum and CSF.

Fig 1 shows the results of the concentration measurements of fetuin-A in CSF and serum. The correlation of fetuin-A in serum (y-axis, mg/ml) with an elevated C-reactive protein concentration (x-axis, (A)) and the presence of neuroinflammatory disease (x-axis, (B)) is shown. The relation between fetuin-A in CSF (y-axis, μg/ml) and a disruption of the blood-CSF barrier (x-axis) is displayed in (C). (D) and (E) show the connection of the serum fetuin-A/total serum protein ratio (y-axis) with an intrathecal IgG synthesis (x-axis, (D)) and the disruption of the blood-CSF barrier (x-axis, (E)). In (F), the link between the CSF fetuin-A/total CSF protein ratio (y-axis) and age (x-axis, years) is shown as a scatter plot. (G) and (H) show the correlation between the CSF fetuin-A/serum fetuin-A quotient QFet (y-axis, x103) and the CSF albumin/serum albumin quotient QAlb (x-axis, x103, (G)) and the presence of a blood-CSF barrier disruption (x-axis, (H)). (G) represents a scatter blot double logarithmically. Values of the control group (black) and inflammatory group (white) are plotted separately. (H) shows a boxplot.</p

Multiple linear regression.

Predictors for fetuin-A concentration (μg/ml) in cerebrospinal fluid. (PDF)</p

Clinical data of samples for phosphorylation studies.

Clinical data of samples for phosphorylation studies.</p

Multiple linear regression.

Predictors for relative CSF phosphofetuin-A concentrations. (PDF)</p

Multiple linear regression.

Predictors for serum fetuin-A/serum total protein ratio. (PDF)</p

Glycosylation patterns of fetuin-A in CSF and serum.

Fig 2 illustrates the results of the glycosylation studies of fetuin-A. (A) and (B) show the glycosylation patterns of fetuin-A with (A) and without (B) double bands. On each membrane, lane 1 shows undigested fetuin-A, lane 2 shows fetuin-A after PNGase-F digestion and lane 3 shows fetuin-A after sialidase-Au digestion. (C) compares the occurrence of double bands in the control group and inflammatory group as a bar chart. (D) displays the kinetic of PNGase-F digestion in serum and CSF. Lane 1 shows undigested fetuin-A, lane 2 shows the pattern after 1 minute of digestion, lane 3 displays the pattern after 10 minutes of digestion and lane 4 shows fetuin-A after 3 hours of PNGase-F digestion.</p

Phosphorylation patterns of fetuin-A in CSF and serum.

Fig 3 displays the results of the statistical analysis of the phosphorylation studies. (A) and (B) show relative CSF phosphofetuin-A concentrations (y-axis, %) in comparison to the CSF albumin/serum albumin quotient (A, x-axis, x103) and in the presence of neuroinflammatory diseases (B). (C) and (D) show absolute phosphofetuin-A levels in CSF (y-axis, μg/ml) in comparison to the CSF albumin/serum albumin quotient ((C), x-axis, x103) and a blood-CSF barrier disruption ((D), x-axis). (E) and (F) display the results of the linear regression with the relative CSF phosphofetuin-A/relative serum phosphofetuin-A quotient. This quotient is shown on the y-axis. (E) shows a scatter plot with the CSF albumin/serum albumin quotient (x-axis, x103). In (E) the results are shown divided into control group (black) and inflammatory group (white). (F) shows the correlation of the CSF phosphofetuin-A/serum phosphofetuin-A quotient with the presence of a neuroinflammatory disorder (x-axis).</p

Clinicial diagnoses of samples for glycosylation and phosphorylation studies.

Clinicial diagnoses of samples for glycosylation and phosphorylation studies.</p

Concentrations of fetuin-A in CSF and CSF fetuin-A/serum fetuin-A quotient.

S1 Fig shows supplementary results of the concentrations measurements of fetuin-A in CSF and serum. The correlation of CSF fetuin-A (y-axis, μg/ml) with an elevated C-reactive protein concentration (x-axis) is displayed as a boxplot in (A). The connection between the CSF fetuin-A/serum fetuin-A quotient (y-axis, x103) and age (x-axis, years) is displayed as scatter plot in (B). (C) shows the correlation of the CSF fetuin-A/serum fetuin-A quotient (y-axis, x103) with an elevated C-reactive protein concentration (x-axis) as a boxplot. (TIF)</p