2 research outputs found
Modification and Optimization of the Bis-picolylamide-Based Relay Protection for Carboxylic Acids to be Cleaved by Unusual Complexation with Cu<sup>2+</sup> Salts
A simple modification of our recently published protection
scheme for carboxylic acids as amides resulted in a new protecting
group with significantly improved properties. It requires shorter
reaction times for deprotection and allows us to replace CuĀ(OTf)<sub>2</sub> by CuCl<sub>2</sub>, indicating at the same time the importance
of the nature of the anion of the Cu<sup>2+</sup> source. Since the
new scheme fulfills all criteria required for an ideal protection
group it should find widespread application in synthetic organic chemistry
Dye Dynamics in Three-Color FRET Samples
Time-resolved emission data (fluorescence decay and fluorescence
depolarization) of two three-color FoĢrster resonance energy
transfer (tc-FRET) systems consisting of a carbostyril donor (D),
a ruthenium complex (Ru) as relay dye, and a Cy5 derivative (Cy) or,
optionally, an anthraquinone quencher (Q) were carefully analyzed
using advanced distribution analysis models. Thereby, it is possible
to get information on the flexibility and mobility of the chromophores
which are bound to double stranded (ds) DNA. Especially the distance
distribution based on the analysis of the fluorescence depolarization
is an attractive approach to complement data of fluorescence decay
time analysis. The distance distributions extracted from the experimental
data were in excellent agreement with those determined from accessible
volume (AV) simulations. Moreover, the study showed that for tc-FRET
systems the combination of dyes emitting on different time scales
(e.g., nanoseconds vs microseconds) is highly beneficial in the distribution
analysis of time-resolved luminescence data in cases where macromolecules
such as DNA are involved. Here, the short lifetimes can yield information
on the rotation of the dye molecule itself and the long lifetime can
give insight in the overall dynamics of the macromolecule