Informed Consent to Address Trust, Control, and Privacy Concerns in User Profiling

More and more, services and products are being personalised or\ud tailored, based on user-related data stored in so called user profiles or user\ud models. Although user profiling offers great benefits for both organisations and\ud users, there are several psychological factors hindering the potential success of user profiling. The most important factors are trust, control and privacy\ud concerns. This paper presents informed consent as a means to address the\ud hurdles trust, control, and privacy concerns pose to user profiling

DNA nanoparticles as ocular drug delivery platform

The field of DNA nanotechnology has progressed rapidly in recent years and now offers facile fabrication of a large variety of nanostructures of different shapes and geometries. In this thesis, short nucleic acid sequences are functionalized with hydrophobic units to form the basis of an ocular drug delivery platform. These DNA amphiphiles self-assemble into micellar nanoparticles (NPs) that exhibit a high affinity to the ocular surface. After selecting the best carrier, loading of the medication can easily be performed by employing drug specific aptamers that are hybridized on the NP. The developed platform shows a significantly prolonged availability of the drug compared to the free medication. In future applications, this allows for a lower dosing regime of the eye drop and a reduced drug concentration, which greatly improve the treatment of ophthalmic diseases

DNA nanoparticles as ocular drug delivery platform

The field of DNA nanotechnology has progressed rapidly in recent years and now offers facile fabrication of a large variety of nanostructures of different shapes and geometries. In this thesis, short nucleic acid sequences are functionalized with hydrophobic units to form the basis of an ocular drug delivery platform. These DNA amphiphiles self-assemble into micellar nanoparticles (NPs) that exhibit a high affinity to the ocular surface. After selecting the best carrier, loading of the medication can easily be performed by employing drug specific aptamers that are hybridized on the NP. The developed platform shows a significantly prolonged availability of the drug compared to the free medication. In future applications, this allows for a lower dosing regime of the eye drop and a reduced drug concentration, which greatly improve the treatment of ophthalmic diseases

United in Diversity? A Study on the Implementation of Sanctions in the European Union

The implementation of European Union (EU) policies has been investigated for several policy areas, but Decisions made under the Common Foreign and Security Policy (CFSP) have rarely been considered. While many CFSP measures are applicable throughout the EU without the need for further action on the domestic level, some Decisions must be implemented by Council Regulations. These Council Regulations adopted with the intent to implement CFSP Decisions have qualities of Directives, which delegate implementing tasks to member states and require transposition. The aim of this article is to investigate whether restrictive measures imposed by the EU are uniformly implemented across the member states, and, if not, to what extent implementation performance varies. We observe significant differences in implementation performance across member states. The findings of this article are twofold. First, we claim that implementation and compliance studies should involve CFSP decisions more systematically. Second, empirical confirmation is provided of how uneven transposition and application occurs also in CFSP matters. This study is based on empirical work that consisted of desk research and semi-structured interviews with national competent authorities of 21 EU member states taking place between March 2020 and January 2021

On the origin of vanillyl alcohol oxidases

Vanillyl alcohol oxidase (VAO) is a fungal flavoenzyme that converts a wide range of para-substituted phenols. The products of these conversions, e.g. vanillin, coniferyl alcohol and chiral aryl alcohols, are of interest for several industries. VAO is the only known fungal member of the 4-phenol oxidising (4PO) subgroup of the VAO/PCMH flavoprotein family. While the enzyme has been biochemically characterised in great detail, little is known about its physiological role and distribution in fungi. We have identified and analysed novel, fungal candidate VAOs and found them to be mostly present in Pezizomycotina and Agaricomycotina. The VAOs group into three clades, of which two clades do not have any characterised member. Interestingly, bacterial relatives of VAO do not form a single outgroup, but rather split up into two separate clades. We have analysed the distribution of candidate VAOs in fungi, as well as their genomic environment. VAOs are present in low frequency in species of varying degrees of relatedness and in regions of low synteny. These findings suggest that fungal VAOs may have originated from bacterial ancestors, obtained by fungi through horizontal gene transfer. Because the overall conservation of fungal VAOs varies between 60 and 30% sequence identity, we argue for a more reliable functional prediction using critical amino acid residues. We have defined a sequence motif P-x-x-x-x-S-x-G-[RK]-N-x-G-Y-G-[GS] that specifically recognizes 4PO enzymes of the VAO/PCMH family, as well as additional motifs that can help to further narrow down putative functions. We also provide an overview of fingerprint residues that are specific to VAOs

On the Putative Detection of z>0 X-ray Absorption Features in the Spectrum of Markarian 421

In a series of papers, Nicastro et al. have reported the detection of z>0 OVII absorption features in the spectrum of Mrk421 obtained with the Chandra Low Energy Transmission Grating Spectrometer (LETGS). We evaluate this result in the context of a high quality spectrum of the same source obtained with the Reflection Grating Spectrometer (RGS) on XMM-Newton. The data comprise over 955ks of usable exposure time and more than 26000 counts per 50 milliAngstrom at 21.6 Angstroms. We concentrate on the spectrally clean region (21.3 < lambda < 22.5 Angstroms) where sharp features due to the astrophysically abundant OVII may reveal an intervening, warm--hot intergalactic medium (WHIM). We do not confirm detection of any of the intervening systems claimed to date. Rather, we detect only three unsurprising, astrophysically expected features down to the Log(N_i)~14.6 (3 sigma) sensitivity level. Each of the two purported WHIM features is rejected with a statistical confidence that exceeds that reported for its initial detection. While we can not rule out the existence of fainter, WHIM related features in these spectra, we suggest that previous discovery claims were premature. A more recent paper by Williams et al. claims to have demonstrated that the RGS data we analyze here do not have the resolution or statistical quality required to confirm or deny the LETGS detections. We show that our careful analysis resolves the issues encountered by Williams et al. and recovers the full resolution and statistical quality of the RGS data. We highlight the differences between our analysis and those published by Williams et al. as this may explain our disparate conclusions.Comment: 19 pages/7 figures/4 tables. 060424 submitted to ApJ 060522 re-submitted following ApJ reques