2 research outputs found
The synthesis of recombinant membrane proteins in yeast for structural studies
- Author
- Publication venue
- 'Elsevier BV'
- Publication date
- 01/02/2016
- Field of study
Get PDFHistorically, recombinant membrane protein production has been a major challenge meaning that many fewer membrane protein structures have been published than those of soluble proteins. However, there has been a recent, almost exponential increase in the number of membrane protein structures being deposited in the Protein Data Bank. This suggests that empirical methods are now available that can ensure the required protein supply for these difficult targets. This review focuses on methods that are available for protein production in yeast, which is an important source of recombinant eukaryotic membrane proteins. We provide an overview of approaches to optimize the expression plasmid, host cell and culture conditions, as well as the extraction and purification of functional protein for crystallization trials in preparation for structural studies
CMS Physics Technical Design Report, Volume II: Physics Performance
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- Abazov V M
- Abbott B
- Abbott B
- Abdullin S
- Abdullin S
- Abdullin S
- Abdullin S
- Abdullin S
- Abdullin S
- Abdullin S Drozdetskiy A
- Abramov V
- Acosta D
- Acosta D
- Acosta D
- Acosta D
- Acosta D
- Adam W Mangano B Speer T Todorov T
- Adloff C
- Aguilar-Saavedra J A Riemann T
- Albrow M G Rostovtsev A
- Alcaraz J
- Aldaya M
- Aldaya M Arce P Caballero J de la Cruz B Garcia-Abia P Hernandez J M Josa M Ruiz E
- Alekhin S
- ALEPH
- Allanach B C
- Allanach B C
- Alwall J
- Anastasiou C
- Andreev V P Cline D.B Otwinowski S
- Arkani-Hamed N
- Arneodo M
- Arneodo M Diehl M
- Asatrian H M Hovhannisyan A Poghosyan V Greub C Hurth T
- Assamagan K A
- ATLAS Collaboration
- ATLAS Collaboration
- Avati V
- Azuelos G
- Baarmand M Hashemi M Nikitenko A
- Baarmand M Hashemi M Nikitenko A
- Baarmand M Mermerkaya H Vodopiyanov I
- Baffioni S
- Bagliesi G Dutta S Gennai S Kalinowski A Konecki M Kotlinski D Moortgat F Nikitenko A Wendland L Wakefield S
- Barbieri R
- Bardin D Y
- Bartalini P
- Bartalini P
- Bartalini P
- Bartalini P Chierici R De Roeck A
- Bartsch V
- Bartsch V Quast G
- Bartsch V Quast G
- Beauchemin P
- Beauchemin P-H
- Bedjidian M
- Bedjidian M Kodolova O
- Beenakker W Hopker R Spira M
- Bejar S Guasch J Sola J
- Belotelov I
- Belotelov I
- Belotelov I
- Belotelov I
- Belotelov I Golutvin I Bourilkov D Lanyov A Rogalev E Savina M Shmatov S
- Belyaev A
- Benedetti D Cucciarelli S Hill C Incandela J Koay S Riccardi C Santocchia A Schmidt A Torre P Weiser C
- Beneke M
- Berezhnoi A V
- Betev B Bourilkov D Mavrodiev S. Shch
- Binoth T
- Binoth T
- Binoth T
- Bityukov S
- Bityukov S I
- Bityukov S I
- Bityukov S I
- Bityukov S Krasnikov N Taperechkina V
- Bloch D
- Bluj M
- Boonekamp M
- Boos E
- Boos E Bunichev V Dudko L Savrin V Sherstnev A
- Bopp F W Engel R Ranft J
- Bourilkov D
- Bourilkov D
- Brigljević V
- Brignole A
- Bruni G
- Buege V
- Buttar C
- Buttar C
- Buttar C M
- Butterworth J Butterworth S Waugh B Stirling W Whalley M
- Cacciari M
- Campbell J
- Campbell J M
- Carena M Heinemeyer S Wagner C Weiglein G
- Catani S
- Catani S
- Cavalli D
- CDF
- CDF Run II Collaboration Abulencia A
- Cerminara G
- Chang C-H
- Chekanov S V
- Chen G Meng X Tao J
- Cheng T P
- Chierici R Dierlamm A
- Chiorboli M Galanti M Tricomi A
- Clerbaux B Mahmoud T Collard C Lemaire M-C Litvin V
- Clerbaux B Mahmoud T Collard C Miné P
- CMS Collaboration
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- CMS Collaboration Acosta D
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- CMS Collaboration Acosta D
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- CMS Collaboration Acosta D
- CMS Collaboration Acosta D
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- CMS Collaboration Gninenko N Kirsanov M Krasnikov N Matveev N
- CMS Collaboration Karafasoulis K Kyriakis A Petrakou H Mazumdar K
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- CMS Collaboration Lethuillier M
- CMS Collaboration Litvin V
- CMS Collaboration Marinelli N
- CMS Collaboration Meschi E
- CMS Collaboration Pieri M
- CMS Collaboration Pieri M
- CMS/TOTEM Collaboration
- Collard C Lemaire M-C
- Collins J C
- CompHEP Collaboration Boos E Bunichev V Dubinin M Dudko L Edneral V Ilyin V Kryukov A Savrin V Semenov A Sherstnev A
- Corcella G
- Cousins R
- Cousins R Mumford J Valuev S
- Cousins R Mumford J Valuev V
- Cousins R Mumford J Valuev V
- Croft R
- Cucciarelli S Konecki M Kotlinski D Todorov T
- Cvetic M
- D'Hondt J
- D'Hondt J Heyninck J Lowette S
- D'Hondt J Heyninck J Lowette S
- D'Hondt J Lowette S Buchmuller O Cucciarelli S Schilling F P Spiropulu M Mehdiabadi S Benedetti D Pape L
- D'Hondt J Lowette S Hammad G Heyninck J Van Mulders P
- D'Hondt J Lowette S Heyninck J Kasselmann S
- Davatz G
- Davatz G
- Davatz G Dittmar M Giolo-Nicollerat A S
- Davatz G Dittmar M Giolo-Nicollerat A-S
- Davatz G Giolo-Nicollerat A Zanetti M
- Davids M
- Dawson S
- de Boer W
- De Roeck A
- De Roeck A Slabospitsky S
- Degrassi G Maltoni F
- Delaere C
- Denegri D Rurua L Stepanov N
- Diaz R A Martinez R Alexis Rodriguez J
- Dighe A S
- Dissertori G
- Dittmar M Dreiner H
- Djouadi A
- Djouadi A
- Djouadi A Ferrag S
- Dobbs M
- Dobbs M A
- Dominici D Dewhirst G Nikitenko A Gennai S Fanò L
- Drollinger V
- Drollinger V Gasparini U Torassa E Zanetti M
- Dubinin M Ilyin V Savrin V
- Dubinin M Litvin V Ma Y Newman H Pieri M
- ECFA/DESY LC Physics Working Group Collaboration Aguilar-Saavedra J A
- Ellis S D
- Engel R Gaisser T K Stanev T Lipari P
- Esen S Harris R
- Esen S Harris R M
- Espinosa J R
- Eynard G
- Favara A Pieri M
- Fernandez J
- Ferro F
- Field R
- Field R D
- Finley C
- Fisher R A
- Foudas C Nikitenko A Takahashi M
- FP420 Collaboration Albrow M G
- Frixione S
- Frixione S
- Frixione S
- Frixione S
- Frixione S Webber B R
- Futyan D Fortin D Giordano D
- Gennai S
- Gennai S Nikitenko A Wendland L
- Giele W
- Giolo-Nicollerat A-S
- Gleisberg T
- Gleisberg T
- Glosser C J
- Godfrey S
- Goldberg D E
- Gorishnii S G
- Goulianos K
- Graham D J
- Grenier P
- Grothe M
- Gumus K Akchurin N Esen S Harris R M
- Hagiwara K
- Han T Logan H E Wang L-T
- Harlander R V
- Harlander R V
- Harlander R V Hofmann F
- Harris C M
- Hauser J
- Haywood S
- Heavy Flavor Averaging Group Collaboration Barberio E
- Heinemeyer S
- Heinemeyer S
- Heinson A
- Heister A Kodolova O Konoplianikov V Petrushanko S Rohlf J Tully C Ulyanov A
- Hewett J L
- Higgs Working Group Collaboration Assamagan K
- Higgs Working Group Collaboration Carena M
- Hinchliffe I
- Hof C Hebbeker T Hoepfner K
- Holland J H
- Iancu Edmond Venugopalan Raju
- James E Maravin Y Mulders M Neumeister N
- Jiang Y
- Kalinowski A
- Kalinowski A Konecki M Kotlinski D
- Kalinowski A Konecki M Kotliński D
- Kalliopuska J
- Karafasoulis K
- Karimaki V
- Karsch Frithjof
- Kauer N
- Kharchilava Avto
- Kinnunen R
- Kinnunen R Denegri D
- Kinnunen R Lehti S
- Kisselev A
- Kluge T Rabbertz K Wobisch M
- Knowles I G
- Kodolova O Bedjidian M Petrouchanko S
- Konoplianikov V Kodolova O Ulyanov A
- Krauss F
- Kreuzer P
- Kulesza A
- Kunori S Kinnunen R Nikitenko A
- Kyriazopoulou S Markou C
- Lane K D
- Lane K D
- Langenegger U Spira M Starodumov A Trueb P
- Larios F
- Lehmann E L
- Lehti S
- Lehti S
- Lehti S
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- LEP Higgs Working Group for Higgs boson searches Collaboration
- Lethuillier M
- Lethuillier M Agram J-L Baty C Gascon-Shotkin S Perries S Ravat O
- LHC/LC Study Group Collaboration Weiglein G
- Lokhtin I P
- Lokhtin I P Snigirev A M
- Lokhtin I P Snigirev A M
- Lonnblad L
- Lowette S D'Hondt J Heyninck J Vanlaer P
- Lowette S D'Hondt J Heyninck J Vanlaer P
- Lowette S D'Hondt J Vanlaer P
- Lykken J D
- Ma E Sarkar U
- Maltoni F
- Maltoni F
- Mangano M
- Mangano M L
- Mangeol D Goerlach U
- Martin S P
- Mathews P
- Meridiani P Paramatti R
- Mohapatra R N
- Monk J Pilkington A
- Moretti S Nolten M R Ross D A
- Mrenna S
- Muon Collaboration Bennett G W
- Murayama H Watanabe I Hagiwara K
- Nason P
- Nikitenko A Quast G Ciulli V
- O'Neill R
- Oljemark F
- Paige F E
- Petrov A
- Pi H
- Pi H Avery P Rohlf J Tully C Kunori S
- Piotrzkowski K
- Plehn T
- Prokofiev K Speer T
- Pukhov A
- Pumplin J
- Ravat O
- Read A
- Read A L
- Rizzi A Palla F Segneri G
- Roberfroid V
- Roesler S Engel R Ranft J
- Ryutin R
- Santocchia A
- Seez C
- Sjostrand T Lonnblad L Mrenna S
- Sjostrand T Lonnblad L Mrenna S Skands P
- Slabospitsky S R
- Speer T
- Spira M
- Spira M
- Starinets Andrei O
- Steinhauser M
- Strohmer R
- Stump D
- Stump D R
- Tevatron Electroweak Working Group Collaboration
- Tevatron Electroweak Working Group Collaboration
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- TOTEM Collaboration
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- Vanlaer P
- Vos M Palla F
- Weiser C
- Weng J
- Whalley M R Bourilkov D Group R C
- Wilks S S
- Wobisch M
- Wu P
- Wulz C-E
- Yao W-M
- Yeh P
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- Zykunov V
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