Imaging of Ultra-Weak Photon Emission in a Rheumatoid Arthritis Mouse Model

Analytical BioScience

Traditional Chinese Medicine-Based Subtyping of Early-Stage Type 2 Diabetes Using Plasma Metabolomics Combined with Ultra-Weak Photon Emission

The prevalence of type 2 diabetes mellitus (T2DM) is increasing rapidly worldwide. Because of the limited success of generic interventions, the focus of the disease study has shifted toward personalized strategies, particularly in the early stages of the disease. Traditional Chinese medicine (TCM) is based on a systems view combined with personalized strategies and has improved our knowledge of personalized diagnostics. From a systems biology perspective, the understanding of personalized diagnostics can be improved to yield a biochemical basis for such strategies; for example, metabolomics can be used in combination with other system-based diagnostic methods such as ultra-weak photon emission (UPE). In this study, we investigated the feasibility of using plasma metabolomics obtained from 44 pre-T2DM subjects to stratify the following TCM-based subtypes: Qi-Yin deficiency, Qi-Yin deficiency with dampness, and Qi-Yin deficiency with stagnation. We studied the relationship between plasma metabolomics and UPE with respect to TCM-based subtyping in order to obtain biochemical information for further interpreting disease subtypes. Principal component analysis of plasma metabolites revealed differences among the TCM-based pre-T2DM subtypes. Relatively high levels of lipids (e.g., cholesterol esters and triglycerides) were important discriminators of two of the three subtypes and may be associated with a higher risk of cardiovascular disease. Plasma metabolomics data indicate that the lipid profile is an essential component captured by UPE with respect to stratifying subtypes of T2DM. The results suggest that metabolic differences exist among different TCM-based subtypes of pre-T2DM, and profiling plasma metabolites can be used to discriminate among these subtypes. Plasma metabolomics thus provides biochemical insights into system-based UPE measurements. (C) 2019 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company.Analytical BioScience

Ultraweak Photon Emission as a Non-Invasive Health Assessment: A Systematic Review

Analytical BioScience

Иберийско-кавказское языкознание N42

Analytical BioScience

Statistical analysis of the spontaneously emitted photon signals from palm and dorsal sides of both hands in human subjects

Photon signals emitted spontaneously from dorsal and palm sides of both hands were recorded using 6000 time windows of size T=50. ms in 50 healthy human subjects. These photon signals demonstrated universal behaviour by variance and mean. The data sets for larger time windows up to T=50. s were obtained by merging the data recorded with T=50. ms. The behaviour of Fano factor regarding different window sizes was investigated. The Fano factor hovered around one in signals up to T=3. s and increased slowly with the increase in window size. This indicated super-Poissonian distribution of photo counts. The Fano factor curve F(T) obtained by averaging all subjects and locations had a characteristic shape. Data suggest that the shape is essentially a combination of a smaller sub-population of individuals with sub-Poissonian and a larger sub-population with predominantly super-Poissonian photo count distribution. Averaging the data obtained by randomly shuffling observed data sets was flat and did not show any structure with T. The same applied both to the observed background data sets and the data sets obtained by randomly shuffling background in 50 measurements. The Fano factor was also flat in 50 measurements documented both by a standard LED as well as its shuffled data sets. The structure in the shape F(T) is characteristic of human signals. It may contain valuable information about metabolic processes and may have diagnostic relevance. © 2010 Elsevier B.V

Delayed luminescence: an experimental protocol for Chinese herbal medicines

Analytical BioScience

Measuring ultra-weak photon emission as a non-invasive diagnostic tool for detecting early-stage type 2 diabetes: A step toward personalized medicine

Analytical BioScience

Cellular glutathione levels in HL-60 cells during respiratory burst are not correlated with ultra-weak photon emission

Analytical BioScience

A Chinese literature overview on ultra-weak photon emission as promising technology for studying system-based diagnostics.

To present the possibilities pertaining to linking ultra-weak photon emission (UPE) with Chinese medicine-based diagnostics principles, we conducted a review of Chinese literature regarding UPE with respect to a systems view of diagnostics. Data were summarized from human clinical studies and animal models published from 1979 through 1998. The research fields can be categorized as follows: (1) human physiological states measured using UPE; (2) characteristics of human UPE in relation to various pathological states; and (3) the relationship between diagnosis (e.g., Chinese syndromes) and the dynamics of UPE in animal models. We conclude that UPE has clear potential in terms of understanding the systems view on health and disease as described using Chinese medicine-based diagnostics, particularly from a biochemistry-based regulatory perspective. Linking UPE with metabolomics can further bridge biochemistry-based Western diagnostics with the phenomenology-based Chinese diagnostics, thus opening new avenues for studying systems diagnostics in the early stage of disease, for prevention-based strategies, as well as for systems-based intervention in chronic disease.Analytical BioScience

Pharmacological targeting of ROS reaction network in myeloid leukemia cells monitored by ultra-weak photon emission

Acute myeloid leukemia (AML) is a blood cancer that is caused by a disorder of the process that normally generates neutrophils. Function and dysfunction of neutrophils are key to physiologic defense against pathogens as well as pathologies including autoimmunity and cancer. A major mechanism through which neutrophils contribute to health and disease is oxidative burst, which involves rapid release of reactive oxygen species (ROS) generated by a chemical reaction network catalyzed by enzymes including NADPH oxidase and myeloperoxidase (MPO). Due to the involvement of neutrophil-derived reactive oxygen species in many diseases and importance of NADPH oxidase and MPO-mediated reactions in progression and treatment of myeloid leukemia, monitoring this process and modulating it by pharmacological interventions is of great interest. In this work, we have evaluated the potential of a label-free method using ultra-weak photon emission (UPE) to monitor ROS production in neutrophil-like HL60 myeloid leukemia cells. Suppression of ROS was achieved by several drug candidates that target different parts of the reaction pathway. Our results show that UPE can report on ROS production as well as suppression by pharmacological inhibitors. We find that UPE is primarily generated by MPO catalyzed reaction and thus will be affected when an upstream reaction is pharmacologically modulated