37 research outputs found
High-quality permanent draft genome sequence of Rhizobium leguminosarum bv. viciae strain GB30; an effective microsymbiont of Pisum sativum growing in Poland
Rhizobium leguminosarum bv. viciae GB30 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Pisum sativum. GB30 was isolated in Poland from a nodule recovered from the roots of Pisum sativum growing at Janow. GB30 is also an effective microsymbiont of the annual forage legumes vetch and pea. Here we describe the features of R. leguminosarum bv. viciae strain GB30, together with sequence and annotation. The 7,468,464 bp high-quality permanent draft genome is arranged in 78 scaffolds of 78 contigs containing 7,227 protein-coding genes and 75 RNA-only encoding genes, and is part of the GEBA-RNB project proposal
High-quality permanent draft genome sequence of Rhizobium leguminosarum bv. viciae strain GB30; an effective microsymbiont of Pisum sativum growing in Poland
Rhizobium leguminosarum bv. viciae GB30 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Pisum sativum. GB30 was isolated in Poland from a nodule recovered from the roots of Pisum sativum growing at Janow. GB30 is also an effective microsymbiont of the annual forage legumes vetch and pea. Here we describe the features of R. leguminosarum bv. viciae strain GB30, together with sequence and annotation. The 7,468,464 bp high-quality permanent draft genome is arranged in 78 scaffolds of 78 contigs containing 7,227 protein-coding genes and 75 RNA-only encoding genes, and is part of the GEBA-RNB project proposal
High-quality permanent draft genome sequence of Rhizobium leguminosarum bv. viciae strain GB30; an effective microsymbiont of Pisum sativum growing in Poland
Rhizobium leguminosarum bv. viciae GB30 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Pisum sativum. GB30 was isolated in Poland from a nodule recovered from the roots of Pisum sativum growing at Janow. GB30 is also an effective microsymbiont of the annual forage legumes vetch and pea. Here we describe the features of R. leguminosarum bv. viciae strain GB30, together with sequence and annotation. The 7,468,464 bp high-quality permanent draft genome is arranged in 78 scaffolds of 78 contigs containing 7,227 protein-coding genes and 75 RNA-only encoding genes, and is part of the GEBA-RNB project proposal
Influence of quorum sensing signal molecules on biofilm formation in Proteus mirabilis O18
The influence of basis of quorum sensing molecules on Proteus strains is much less known as compared to Pseudomonas or Escherichia. We have previously shown that a series of acylated homoserine lactones (acyl-HSL) does not influence the ureolytic, proteolytic, or hemolytic abilities, and that the swarming motility of Proteus mirabilis rods is strain specific. The aim of the presented study was to find out if the presence of a series of acyl-HSL influences biofilm formation of P. mirabilis laboratory strain belonging to O18 serogroup. This serogroup is characterized by the presence of a unique non-carbohydrate component, namely phosphocholine. Escherichia coli and P. mirabilis O18 strains used in this work contains cloned plasmids encoding fluorescent protein genes with constitutive gene expression. In mixed biofilms in stationary and continuous flow conditions, P. mirabilis O18 overgrow whole culture. P. mirabilis O18 strain has genetically proved a presence of AI–2 quorum sensing system. Differences in biofilm structure were observed depending on the biofilm type and culture methods. From tested acylated homoserine lactones (BHL, HHL, OHL, DHL, dDHL, tDHL), a significant influence had BHL on thickness, structure, and the amount of exopolysaccharides produced by biofilms formed by P. mirabilis O18 pDsRed2
Influence of phosphate and ammonia on the growth, exopolysaccharide production and symbiosis of Rhizobium leguminosarum bv. Trifolii TA1 with clover (Trifolium pratense)
The
Rhizobium
-legume interaction is sensitive to a number of environmental factors, among which phosphate (Pi) and ammonium availability are the most important. We investigated the effect of Pi and ammonia concentration on exopolysaccharide production and symbiosis
Trifolium pratense
with
Rhizobium leguminosarum
bv.
trifolii
TA1 (RtTA1). The optimal Pi concentration in the bacterial growth medium for RtTA1 growth and exopolysaccharide production was in the range from 0.9 mM to 8.1 mM. Independently of Pi concentration, ammonium (NH
4
Cl) concentration above 8.1 mM in the culture medium significantly decreased EPS production, indicating a regulatory role of this nutrient on the EPS production in the RtTA1 strain. Pi availability has a beneficial effect on both partners of symbiosis. Pi concentration in the plant medium in the range from 1.7 mM to 5 mM was optimal for nodule formation, nodule occupancy and nitrogen fixation ability. Despite of
T. pratense
cv. Bryza tolerance on high Pi concentration, 20 mM Pi occurs to be nearly phytotoxic, which negatively affects almost all symbiotic parameters. Large amounts of starch were accumulated in the nodules formed by clover grown on medium containing high Pi concentration
Usefulness of Nod preparation (LCOs) use to presowable dressing of pea seeds (Pisum sativum L.)
Przeprowadzone dotychczas badania nad symbiozą roślin bobowatych i rizobiów odkryły podłoże genetyczne tego zjawiska
i pozwoliły na zidentyfikowanie licznych metabolitów roślinnych i bakteryjnych zaangażowanych w proces nawiązania symbiozy
i rozwój brodawek korzeniowych. Jednym z takich związków są bakteryjne czynniki Nod (LCOs), uznawane za cząstki
sygnalne uczestniczące w wymianie informacji pomiędzy bakterią i rośliną, wpływające między innymi na powstawanie
brodawek korzeniowych na roślinach bobowatych. Związki te, na drodze od producenta do organizmu docelowego, często
podlegają procesom powodującym zmniejszenie ich stężenia, dyfundują lub rozpadają się w roztworze glebowym oraz są
degradowane przez mikroorganizmy glebowe. Można zatem przypuszczać, że niedostateczna ich ilość jest przyczyną mało
wydajnej symbiozy, co znacznie ogranicza zaopatrzenie roślin w azot i zmniejsza ich plonowanie. W badaniach określano
wpływ stosowania czynników Nod na ontogenezę, kształtowanie fizjologicznych wskaźników wzrostu oraz plonowanie
dwóch zróżnicowanych morfologicznie odmian grochu siewnego (genotyp o normalnym ulistnieniu i genotyp wąsolistny –
afila). Zastosowanie czynników Nod zwiększyło m.in. liczbę i masę brodawek korzeniowych, co w konsekwencji prowadziło
do wytwarzania większej masy organów wegetatywnych i generatywnych przez rośliny grochu siewnego.The studies conducted till now on symbiosis of papilionaceous plants and rhizobia discovered the genetic background of
this phenomenon and allowed identification of numerous plant and bacteria metabolites involved at process of starting of
symbiosis and development of nodules. One of these compounds are bacteria Nod factors (LCOs), recognised as signal particles
participating in change of information between a bacteria and a plant, effected among others on nodules formation on
papilionaceous plants. These compounds are often submitted to processes which cause decrease of their concentration on
the road from producer to destined organism, diffuse or break-up in the soil solution and are degraded by soil microorganisms.
It may be assumed that their insufficient amount is a cause of low efficient symbiosis, what considerably limits plant
supply with nitrogen and decreases their yielding. In this studies it was evaluated an effect of Nod factors use on ontogenesis,
forming of physiological growth indexes and yielding of two morphological differentiated pea varieties (genotype with
normal foliage and narrow leaved genotype - afila type). Using of Nod factors increased among others number and weight
of nodules, what in a consequence led to production of greater mass of vegetative and generative organs by pea plants
Poszukiwanie ekologicznych metod zwiększenia wydajności symbiotycznego wiązania azotu przez groch siewny (Pisum sativum L.)
Conducted till now studies on symbiosis of papilionaceous plants and rhizobia discovered the genetic background of this
phenomenon and allowed to identify numerous plant and bacteria metabolites involved in the process of starting of
symbiosis and development of nodules. One of this compounds are flavonoids, recognised as signal particles participating
in information exchange between a bacteria and a plant, affecting among others nodules formation on papilionaceous
plants. These compounds are often submitted to processes which decrease their concentration on the way from a producer
to a destined organism, they diffuse or break-up in the soil solution and are degraded by soil microorganisms. It can be then
assumed that their insufficient amount is a cause of low efficient symbiosis, what considerably limits plant supply with
nitrogen and decreases their yielding. An effect of use of flavonoid extract obtained from germinating seeds on ontogenesis,
forming of physiological growth indexes and yielding of pea was evaluated in these studies. Application of a flavonoid
preparation increased, among others, the number and weight of nodules and activity of nitrogenase, what in a consequence
led to production of greater mass of vegetative and generative organs by pea plants.Prowadzone dotychczas badania nad symbiozą roślin motylkowatych i rizobiów odkryły podłoże genetyczne tego zjawiska i
pozwoliły na zidentyfikowanie licznych metabolitów roślinnych i bakteryjnych zaangażowanych w proces nawiązania
symbiozy i rozwój brodawek korzeniowych. Jednym z takich związków sa flawonoidy, uznawane za cząstki sygnalne
uczestniczące w wymianie informacji pomiędzy bakterią i rośliną, wpływające między innymi na powstawanie brodawek
korzeniowych na roślinach motylkowatych. Związki te często podlegają procesom powodującym zmniejszenie ich stężenia
na drodze od producenta do organizmu docelowego, dyfundują lub rozpadają się w roztworze glebowym oraz są
degradowane przez mikroorganizmy glebowe. Można zatem przypuszczać, że niedostateczna ich ilość jest przyczyną mało
wydajnej symbiozy, co znacznie ogranicza zaopatrzenie roślin w azot i zmniejsza ich plonowanie. W badaniach określano
wpływ stosowania wyciągu flawonoidowego z kiełkujących nasion na ontogenezę, kształtowanie fizjologicznych wskaźników
wzrostu oraz plonowanie grochu siewnego. Zastosowanie preparatu flawonoidowego zwiększyło między innymi liczbę i
masę brodawek korzeniowych oraz aktywność nitrogenazy, co w konsekwencji prowadziło do wytwarzania większej masy
organów wegetatywnych i generatywnych przez rośliny grochu siewnego
Nod factors improve the nitrogen content and rhizobial diversity of faba bean and alter soil dehydrogenase, protease, and acid phosphomonoesterase activities
Nod factors produced by rhizobia are one of the
most important signals involved in symbiotic associations involving
legumes. A field trial was performed to assess the symbiotic
activity, rhizosphere biological parameters, and plant biomass
of faba bean (Vicia faba L.) treated with Nod factors. The soil
was a Haplic Luvisol derived from loess. The faba bean seeds
(cv. Granit) were soaked with an Nod factors solution (260 nM)
or water (control) and sown. At the flowering stage, the genetic
diversity of rhizobia (based on PCR-RFLP profiles and the
sequencing of the 16-23S rDNA and nodD gene), nitrogenase
activity (acetylene reduction assay), and nodule biomass were
evaluated. Nitrogen yield and plant biomass were determined at
the flowering and maturity stages. Rhizosphere soil was examined
during plant growth in relation to the activities of dehydrogenase,
protease, urease, and acid phosphomonoesterase. The results indicated
that the application of the Nod factors improved nitrogenase
activity (by 74-80%, depending on the parameter analysed) and
increased the genetic diversity of rhizobia inhabiting root nodules,
plant nitrogen content (by 16.8%, at maturity), and seed protein
yield (by 14.6%). The rhizobial population became more heterogeneous
under the influence of the Nod factors than it was for the
control (12 and 7 specific genotypes, respectively). At the flowering
stage, Nod factors enhanced dehydrogenase, protease, and
acid phosphomonoesterase activities by 46, 36 and 9%, respectively.
The results revealed the positive effect of Nod factors at
reducing water deficiency effects during a growing season with
a short-term rainfall deficit