Seasonal Transfer and Net Accumulation of Suspended Sediment in the Yellow and East China Seas

Sediment Transport and Morphodynamic

Adaptive backstepping control for optimal descent with embedded autonomy

Using Lyapunov stability theory, an adaptive backstepping controller is presented in this paper for optimal descent tracking. Unlike the traditional approach, the proposed control law can cope with input saturation and failure which enables the embedded autonomy of lander system. In addition, this control law can also restrain the unknown bounded terms (i.e., disturbance). To show the controller’s performance in the presence of input saturation, input failure and bounded external disturbance, simulation was carried out under a lunar landing scenario

Emergent Decision-Making Practices In Technology-Supported Self-Organizing Distributed Teams

We seek to identify work practices that make technology-supported, self-organizing, distributed (or virtual) teams (TSSODT for short) effective in producing outputs satisfactory to their sponsors, meeting the needs of their members, and continuing to function. A particularly important practice for team effectiveness is decision making: are the right decisions made at the right time to get the work done in a way that satisfies team sponsors, keeps contributors happy and engaged, and enables continued team success? In this research-in-progress paper, we report on an inductive qualitative analysis of 120 decision episodes taken by two Free/Libre Open Source Software development teams. Our analysis revealed differences in decision-making practices that seem to be related to differences in overall team effectiveness

Utilization of H-reversal Trajectory of Solar Sail for Asteroid Deflection

Near Earth Asteroids have a possibility of impacting with the Earth and always have a thread on the Earth. This paper proposes a way of changing the trajectory of the asteroid to avoid the impaction. Solar sail evolving in a H-reversal trajectory is utilized for asteroid deflection. Firstly, the dynamics of solar sail and the characteristics of the H-reversal trajectory are analyzed. Then, the attitude of the solar sail is optimized to guide the sail to impact with the object asteroid along a H-reversal trajectory. The impact velocity depends on two important parameters: the minimum solar distance along the trajectory and lightness number. A larger lightness number and a smaller solar distance lead to a higher impact velocity. Finally, the deflection capability of a solar sail impacting with the asteroid along the H-reversal is discussed. The results show that a 10 kg solar sail with a lead-time of one year can move Apophis out of a 600-m keyhole area in 2029 to eliminate the possibility of its resonant return in 2036

Metagenomic and metatranscriptomic analyses reveal activity and hosts of antibiotic resistance genes in activated sludge

This is the author accepted manuscript.Wastewater treatment plants (WWTPs) are a source and reservoir for subsequent spread of various antibiotic resistance genes (ARGs). However, little is known about the activity and hosts of ARGs in WWTPs. Here, we utilized both metagenomic and metatranscriptomic approaches to comprehensively reveal the diversity, abundance, expression and hosts of ARGs in activated sludge (AS) from three conventional WWTPs in Taiwan. Based on deep sequencing data and a custom-made ARG database, a total of 360 ARGs associated with 24 classes of antibiotics were identified from the three AS metagenomes, with an abundance range of 7.06 × 10−1–1.20 × 10−4 copies of ARG/copy of 16S rRNA gene. Differential coverage binning analysis revealed that >22 bacterial phyla were the putative hosts of the identified ARGs. Surprisingly, genus Mycobacterium and family Burkholderiaceae were observed as multi-drug resistant harboring 14 and 50 ARGs. Metatranscriptome analysis showed 65.8% of the identified ARGs were being expressed, highlighting that ARGs were not only present, but also transcriptionally active in AS. Remarkably, 110 identified ARGs were annotated as plasmid-associated and displayed a close to two-fold increased likelihood of being transcriptionally expressed compared to those ARGs found exclusively within bacterial chromosomes. Further analysis showed the transcript abundance of aminoglycoside, sulfonamide, and tetracycline resistance genes was mainly contributed by plasmid-borne ARGs. Our approach allowed us to specifically link ARGs to their transcripts and genetic context, providing a comprehensive insight into the prevalence, expression and hosts of ARGs in AS. Overall, results of this study enhance our understanding of the distribution and dissemination of ARGs in WWTPs, which benefits environmental risk assessment and management of ARB and ARGsEuropean Union's Horizon 202

Functional characterization of the mitochondrial 12S rRNA C1494T mutation associated with aminoglycoside-induced and non-syndromic hearing loss

In this study, we report the biochemical characterization of the deafness-associated mitochondrial 12S rRNA C1494T mutation using 27 cybrid cell lines constructed by transferring mitochondria from 9 lymphoblastoid cell lines derived from a Chinese family into human mitochondrial DNA (mtDNA)-less (ρ°) cells. Six cybrids derived from two asymptomatic members, and nine cybrids derived from three symptomatic members of the Chinese family carrying the C1494T mutation exhibited ∼38 and 43% decrease in the rate of mitochondrial protein labeling, respectively, compared with twelve cybrids derived from four Chinese control individuals. These defects are apparently a primary contributor to significant reductions in the rate of overall respiratory capacity or the rate of malate/glutamate promoted respiration, or succinate/G3P-promoted respiration, or TMPD/ascorbate-promoted respiration in mutant cybrid cell lines derived from either symptomatic or asymptomatic individuals. Furthermore, the very significant/nearly identical increase in the ratio of doubling times in DMDM medium in the presence/absence of high concentration of paromomycin was observed in symptomatic or asymptomatic cybrid cell lines carrying the C1494T mutation as compared with the average rate in control cell lines. These observations provide the direct biochemical evidences that the C1494T mutation is a pathogenic mtDNA mutation associated with aminoglycoside-induced and non-syndromic hearing loss. In addition, these data provide the first biochemical evidence that nuclear background plays a critical role in the phenotypic manifestation of non-syndromic hearing loss and aminoglycoside toxicity associated with the C1494T mutation

Response Surface Methodology to Optimize Enzymatic Preparation of Deapio-Platycodin D and Platycodin D from Radix Platycodi

In the present work, we reported the enzymatic preparation of deapio-platycodin D (dPD) and platycodin D (PD) optimized by response surface methodology (RSM) from Radix Platycodi. During investigation of the hydrolysis of crude platycosides by various glycoside hydrolases, snailase showed a strong ability to transform deapio-platycoside E (dPE) and platycoside E (PE) into dPD and PD with 100% conversion. RSM was used to optimize the effects of the reaction temperature (35–45 °C), enzyme load (5–20%), and reaction time (4–24 h) on the conversion process. Validation of the RSM model was verified by the good agreement between the experimental and the predicted values of dPD and PD conversion yield. The optimum preparation conditions were as follows: temperature, 43 °C; enzyme load, 15%; reaction time, 22 h. The biotransformation pathways were dPE→dPD3→dPD and PE→PD3→PD, respectively. The determined method may be highly applicable for the enzymatic preparation of dPD and PD for medicinal purposes and also for commercial use

Micro- and nano-fluidics around HAB cells

Have you ever wondered how algae stay so clean? Most flowering-plant leaves also stay clean. Under air, films of water and “dirt” are repelled. Repulsion forces the water into droplets that easily roll off because these leaves are covered in hydrophobic nm- to µm- sized grooves and pillars, producing superhydrophobicity (SH) at the surface. Similarly, most algal cells bear a glycocalyx of organic fibrils that give surface structure, and are often hydrophobic. Glycocalyxes serve many functions, but whether they produce SH is poorly known. SH coatings are being developed to prevent fouling of ships and aquaculture structures without using toxins, so this technology could help understand how algae defeat fouling. Glycocalyxes are composed of exopolymeric secretions (EPS), and algae sometimes make the water more viscous using this tightly and more loosely bound EPS. EPS is also sometimes sticky. SH cuticles on copepods may change ambient fluid microdynamics by allowing slip at their surfaces, and facilitate filter feeding. By managing ambient viscosity and surface properties including slipping and sticking, algae may have the tools to engineer ambient fluidics and stay clean and unfouled