2 research outputs found
EKSPRESI PROTEIN P53 DAN HSP70 PADA SEL PUNCA KARSINOMA NASOFARING YANG RESISTEN TERHADAP RADIOTERAPI
Latar belakang: Pada penderita Karsinoma Nasofaring (KNF) masih sering ditemukan kekambuhan meskipun sudah mendapat terapi yang lengkap. Penelitian terbaru membuktikan bahwa kekambuhan disebabkan oleh sel punca KNF yang resisten terhadap radioterapi. Mekanisme resistensi sel punca kanker terhadap radioterapi diduga karena hambatan terhadap apoptosis dan atau memicu proliferasi. Hambatan terhadap apoptosis disebabkan oleh penurunan protein p53 (wild type), selain over-ekspresi
Hsp70. Tujuan: Menjelaskan mekanisme resistensi sel punca KNF terhadap radioterapi berdasarkan profil ekspresi protein p53(wild type)dan Hsp70.
Metode: Penelitian true experimental dengan menggunakan rancangan randomisasi kelompok kontrol sebelum dan sesudah tes. Kultur sel punca KNF dibagi menjadi dua kelompok, masing-masing 16 sampel. Pada kelompok perlakuan diberikan paparan radioterapi dengan dosis 1,5 Gy menggunakan pesawat Linac, lalu diinkubasi selama 24 jam. Sebelum dan sesudah perlakuan pada kedua kelompok diperiksa ekspresi p53 (wild type) dan Hsp70. Pemeriksaan menggunakan metode flowcytometry.
Hasil: Ekspresi p53 (wild type) antara kelompok perlakuan dan kontrol terdapat perbedaan yang tidak bermakna dengan p=0,576 (p≥0,05). Ekspresi Hsp70, antara kelompok perlakuan dan kontrol terdapat perbedaan yang tidak bermakna dengan p=0,172 (p≥0,05). Kesimpulan: Tidak terdapat perubahan ekspresi p53 (wild type) dan Hsp70 pada sel punca KNF yang resisten terhadap radioterapi
Effectiveness of a multicentre nasopharyngeal carcinoma awareness programme in Indonesia
Objective: To evaluate the effectiveness of a
nasopharyngeal carcinoma (NPC) awareness
programme on the short-term and long-term
improvement of knowledge and referral of patients with
NPC by primary healthcare centres (PHCCs) staff in
Indonesia.
Design: The NPC awareness programme consisted of
12 symposia including a Train-The-Trainer component,
containing lectures about early symptoms and risk
factors of NPC, practical examination and the referral
system for NPC suspects. Before and after training
participants completed a questionnaire. The Indonesian
Doctors Association accredited all activities.
Participants: 1 representative general practitioner
(GP) from each PHCC attended an NPC awareness
symposium. On the basis of the Train-The-Trainer
principle, GPs received training material and were
obligated to train their colleagues in the PHCC.
Results: 703 GPs attended the symposia and trained
1349 staff members: 314 other GPs, 685 nurses and
350 midwives. After the training, respondents’ average
score regarding the knowledge of NPC symptoms
increased from 47 points (of the 100) to 74 points
(p<0.001); this increase was similar between
symposium and Train-The-Trainer component
(p=0.88). At 1½ years after the training, this
knowledge remained significantly increased at 59
points (p<0.001).
Conclusions: The initial results of this NPC
awareness programme indicate that the programme
effectively increases NPC knowledge in the short and
long term and therefore should be continued. Effects
of the improved knowledge on the stage at diagnoses
of the patients with NPC will still need to be
scrutinised. This awareness programme can serve as a
blueprint for other cancer types in Indonesia and for
other developing countries