Responses of Pre-Service Chemistry Teachers at UIN Walisongo Semarang towards e-Learning Management System during Covid-19 Pandemic

Abstract: This study aims to analyze the response of UIN Walisosngo Semarang students to the e-learning system during the COVID-19 pandemic. Quantitative descriptive is the method used in this study with the research instrument in the form of a response questionnaire given to students using the google form. The subjects of this research are 21 pre-service chemistry teacher. The results showed that the e-learning learning media applied during online learning was very beneficial for students. This is evidenced by the student's response to the questions given at the time of filling out the questionnaire by showing quite positive results with an average of 63.81% who answered agree. Based on the data obtained from the research, it shows that e-learning can be applied in online learning at UIN Walisongo Semarang. However, the use of e-learning must always be improved to achieve better learning goals. Keywords: e-learning management system, pre-service chemistry teachers, covid-19 pandemic. Abstrak: Penelitian ini bertujuan untuk menganalisis respons mahasiswa UIN Walisosngo Semarang terhadap sistem e-learning selama pandemi COVID-19. Deskriptif kuantitatif merupakan metode yang digunakan dalam penelitian ini dengan instrument penelitiannya adalah berupa angket respons yang diberikan kepada mahasiswa menggunakan google form. Subjek dari penelitian ini mahasiswa Pendidikan Kimia yang berjumlah 21 (dua puluh satu) orang. Hasil dari penelitian menunjukkan bahwa media pembelajaran e-learning yang diimplementasikan selama pembelajaran daring sangatlah bermanfaat bagi mahasiswa. Hal ini dibuktikan dengan respons mahasiswa terhadap pertanyaan yang diberikan pada saat pengisian angket dengan menunjukkan hasil cukup positif dengan rata-rata yang menjawab setuju sebesar 63,81%. Berdasarkan data yang didapatkan dari penelitian menunjukkan bahwa e-learning dapat diterapkan dalam pembelajaran online di UIN Walisongo Semarang. Namun penggunaan e-learning tersebut harus selalu ditingkatkan untuk mencapai tujuan pembelajaran yang lebih baik lagi Kata kunci: sistem menejemen pembelajaran daring, calon guru kimia, pandemi covid-19. DOI: http://dx.doi.org/10.23960/jppk.v11.i1.2022.1

Preliminary Knowledge Analysis of First-Year Undergraduate Student at UIN Walisongo on Green Chemistry Concept

Abstract: Preliminary Knowledge Analysis of First-Year Undergraduate Student at UIN Walisongo on Green Chemistry Concept. Green chemistry is a form of application of chemistry in reducing or minimizing environmental problems. The implementation of green chemistry in education aims to increase student awareness of the environment, especially early-level students (students in semesters 1 and 3). This study aimed to analyze the knowledge of the green chemistry concept of early-level students of the Chemistry Education Study Program at UIN Walisongo Semarang. The research method used is a qualitative descriptive method. Respondents from the study were early-level students of the Chemistry Education at UIN Walisongo Semarang, totaling 52 people. The selection of respondents was based on the fact that the students of that class had already received a Chemistry Education course for Sustainable Chemistry, which included applying the concept of green chemistry. The average result of the study was 84%, with a positive response. The study results can be used as a reference for further research. Keywords: green chemistry, sustainable development, first-year undergraduate students. Abstrak: Analisis Pengetahuan Awal Mahasiswa Tahun Pertama di UIN Walisongo tentang tentang Konsep Kimia Hijau. Kimia hijau adalah bentuk penerapan kimia dalam mengurangi atau meminimalkan masalah lingkungan. Penerapan green chemistry dalam pendidikan bertujuan untuk meningkatkan kesadaran mahasiswa terhadap lingkungan, khususnya mahasiswa tingkat awal (mahasiswa semester 1 dan 3). Penelitian ini bertujuan untuk menganalisis pengetahuan konsep green chemistry pada mahasiswa tingkat awal Program Studi Pendidikan Kimia UIN Walisongo Semarang. Metode penelitian yang digunakan adalah metode deskriptif kualitatif. Responden penelitian ini adalah mahasiswa tingkat awal Pendidikan Kimia UIN Walisongo Semarang yang berjumlah 52 orang. Pemilihan responden didasarkan pada fakta bahwa siswa kelas tersebut telah mendapatkan mata kuliah Pendidikan Kimia untuk Kimia Berkelanjutan, yang termasuk dalam penerapan konsep green chemistry. Hasil rata-rata penelitian adalah 84%, dengan respon positif. Hasil penelitian tersebut dapat dijadikan sebagai acuan untuk penelitian selanjutnya. Kata kunci: kimia hijau, pengembangan berkelanjutan, mahasiswa tahun pertama.DOI: http://dx.doi.org/10.23960/jppk.v11.i1.2022.1

PENGEMBANGAN FORMULASI BIOMASSA LIMBAH POHON SENGON DAN BONGGOL JAGUNG DALAM INOVASI SENGGOL-CHAR (BIOCHAR LIMBAH POHON SENGON DAN BONGGOL JAGUNG) MELALUI PROSES PIROLISIS

Indonesia adalah negara dengan sumber daya alam kehutanan yang melimpah. Salah satunya potensi kehutanan berupa pohon sengon dan jagung yang berada di desa Pagertoyo Kecamatan Limbangan Kabupaten Kendal. Potensi tersebut menjadi bagian dari komoditi unggulan yang banyak dimanfaatkan dalam sektor industri dan pemenuhan kebutuhan sehari-hari seperti bahan bakar. Namun dalam pemanfaatannya tersebut, masih menyisakan limbah yang berupa limbah gergajian pohon sengon dan bonggol jagung pasca pemanenan. Tujuan dari penelitian ini adalah untuk mengetahui efektivitas penggunaan SENGGOL-CHAR yang bahan bakunya berasal dari kedua jenis limbah tersebut serta memberikan solusi atas krisis pemanasan global yang semakin meningkat. Metode yang dilakukan pada penelitian ini menggunakan metode eksperimental dengan pendekatan penelitian kualitatif. Hasil penelitian menunjukkan bahwa formulasi bahan SENGGOL-CHAR ini mampu mereduksi dan menyerap CO2 hasil dari pembakaran kedua limbah tersebut menggunakan teknologi Pirolisis, dengan kadar unsur C dalam bentuk arang memiliki kadar lebih sedikit dibandingkan ketika dalam bentuk kayu dalam pemenuhan energi masyarakat yang bersifat ramah lingkungan. Serta memiliki kandungan unsur seperti N, P, dan K yang mana sangat berfungsi dalam penyuburan tana

Uji kelayakan video interaktif materi larutan elektrolit dan non elektrolit berbasis green chemistry

Penelitian ini dilatarbelakangi dari penerapan pembelajaran blended learning yang membutuhkan media yang tepat untuk memahami materi larutan elektrolit dan non elektrolit. Media yang dapat digunakan salah satunya adalah video interaktif berisi praktikum materi larutan elektrolit dan non elektrolit berbasis green chemistry. Video interaktif yang didesain dapat diakses secara fleksibel. Praktikum berisi percobaan dengan alat dan bahan yang murah dan mudah ditemukan. Tujuan dalam penelitian ini untuk mengetahui desain serta menguji kelayakan video interaktif larutan elektrolit dan non elektrolit berbasis green chemistry. Metode penelitian yang digunakan adalah penelitian deskriptif kuantitatif. Analisis data menggunakan minifacet rasch model serta diolah secara deskriptif. Desain dari video interaktif berisi kompetensi dasar, tujuan pembelajaran, apersepsi, materi larutan elektrolit dan non elektrolit, serta praktikum larutan elektrolit dan non elektrolit berbasis green chemistry. Analisis uji kelayakan video interaktif yang didesain dilihat dari analisis item dan respon siswa. Analisis uji kelayakan yang dilihat dari analisis item didapatkan hasil bahwa media yang didesain sudah layak karena nilai yang didapatkan sudah sesuai dengan batasan nilai Outfit MnSq yakni 0,5 sampai 1,5, batasan nilai ZStd yakni (-2) sampai 2 dan Pt Mea yakni nilai yang dihasilkan harus positif. Uji kelayakan media yang dilihat dari respon siswa didapatkan hasil bahwa media yang didesain sangat layak dengan skor rata-rata 92,86%

Response to Video Experiments on Identification of Electrolyte and Non-Electrolyte Solutions with Easy, Cheap, and Environmentally Friendly Methods, Tools, and Materials Through Online Media

During a pandemic like this, face-to-face learning is being transferred directly to online learning. Experiments with electrolyte and non-electrolyte solutions require student activity to achieve learning objectives, so an innovative and effective method and media are needed. The purpose of this study was to find out how the response of video viewers that had been distributed through youtube media, both in terms of quality, methods, materials, and chemical content discussed. The research method is by surveying the opinions (responses) of the audience who watched the video, then described qualitatively with graphic data from the response results as support. From the results of the video publication, it was obtained how the response was obtained statistically, and then the statistical data was analyzed based on the average response of each question. It was found that most of the audience agreed on the quality, easy application of the method, cheap materials, and the concept of electrolyte solution being discussed. It was concluded that the experimental media with video in this study could be a solution for teachers to keep teaching their students by practising independently at home with the video content in this study as a guide.

Anticancer Effect of a Typhonium flagelliforme L. in Raji Cells Through Telomerase Expression

Cancer cells have a relatively high telomerase activity compared to normal cells, so the cancer cells have the ability to continue to proliferate and undergo mitosis uncontrolled. Telomerase is an enzyme which responsible for telomere length, a DNA segment that is at the end of eukaryotic cell chromosomes. Telomeres and telomerase play a role in the incidence of carcinogenesis. Natural materials such as taro mice (Typhonium flagelliforme) have potential as anticancer. The purpose of the study was to determine the effects of plant extracts of rat taro on the expression of telomerase in cancer cell Raji. The research method is experimental studies in some form cancerous cell culture cell line, Raji. Used as a control normal cells is Vero cell. The Culture medium used RPMI for Raji cell  and M199 for Vero cell. The study consisted of three groups, control, doses of 1 ½ IC50 and IC50 doses. Expression of telomerase enzyme was measured by the Immunohystochemistry method (IHC). The results showed that the expression of telomerase in cancer cells showed values significantly higher than the normal cells (Vero). Giving mice taro plant extracts (Typhonium flagelliforme) were able to decrease the expression of telomerase significantly in both treatment doses. It was concluded that rodent tuber extract (Typhonium flagelliforme Lodd) can reduce the expression of telomerase in Raji cells, so that the rodent tuber extract (Typhonium flagelliforme Lodd) has potential as an anticancer through the expression of telomerase.Keywords: telomerase, IHC, Typhonium flagelliform

Cytotoxicity assay of Typhonium flagelliforme Lodd against breast and cervical cancer cells

BACKGROUND \ud Cancer is one of the causes of high mortality. Breast and cervical cancers are two of the most frequent cancers affecting women around the world, including Indonesia. Natural materials such as rodent tuber (Typhonium flagelliforme) have anticancer potentials. The rodent tuber extract contains ribosome inactivating proteins (RIPs) capable of cutting the DNA or RNA of cancer cells and blocking the growth of cancer cells. The purpose of this study was to evaluate the cytotoxic effects of Typhonium flagelliforme Lodd extract on HeLa cervical cancer and Michigan Cancer Foundation-7 (MCF-7) breast cancer cells.\ud \ud METHODS\ud Subjects were cultured cell lines of HeLa cells in Rosswell Park Memorial Institute (RPMI) and of MCF-7 cells in Dulbecco’s Minimum Essential Medium (DMEM). Rodent tuber ethanolic extract was diluted in dimethyl sulfoxide (DMSO). The cytotoxicity assay used the 3-(4,5-dimethyl thiazol-2-yl,5-diphenyl) tetrazolium bromide (MTT) method. Absorbance was read in an ELISA reader at a wavelength of 595 nm.\ud \ud RESULTS\ud Rat tuber extract at all dilutions (500; 250; 125; 62.5; 31.25; 15.625;7.81; 3.9 ìg/mL) showed cytotoxic effects against HeLa and MCF-7 cells. Higher concentrations of the extract gave a higher proliferation inhibition effect. Calculated IC50 values of the extract by probit analysis were 30.19 ìg/mL against HeLa cells and 5.586 ìg/mL against MCF-7 cells.\ud \ud CONCLUSIONS\ud Ethanolic extract of Typhonium flagelliforme Lodd has cytotoxic effects against HeLa cells and MCF-7 cells. The cytotoxic effects against MCF-7 cells are greater than the cytotoxic effects against HeLa cells

Ethanolic periwinkle leaf extract reduces telomerase expression in T47D cancer cells

BACKGROUND\ud Cancer cells have a relatively high telomerase activity and a lower p53 protein expression than normal cells, so that cancer cells have the ability to continue to proliferate and do not undergo apoptosis. One of the cancer treatments is chemotherapy using bioactive ingredients from synthesis or isolation of natural materials. One of the plants that have potential as anticancer agent is periwinkle (Catharanthus roseus L). The research objective was to evaluate the effect of ethanolic periwinkle leaf extract against p53 protein and telomerase expression in T47D cancer cells. \ud \ud METHODS\ud An experimental study with controls was conducted involving T47D breast cancer cells. They were divided into 3 groups (control, ½ dose of IC50/26.849 µg/mL, and one dose of IC50/53.699 µg/mL) at a cell density of 1 x 104 cells/well. Expression of p53 and telomerase was measured by the immunohistochemistry method. Data were analyzed using one-way ANOVA followed by a multiple comparison test.\ud \ud RESULTS\ud Periwinkle leaf extract significantly increased p53 protein expression (p<0.05) at both treatment doses, ½ IC50 and IC50, compared to the control group and it highly significantly reduced telomerase expression (p<0.01), in comparison with the control group at both treatment doses. \ud \ud CONCLUSION\ud Periwinkle leaf extract has potential as an anti-breast cancer agent by increasing p53 protein expression and inhibiting telomerase expression.\ud \u

Cytotoxicity assay of Typhonium flagelliforme Lodd against breast and cervical cancer cells

BACKGROUND Cancer is one of the causes of high mortality. Breast and cervical cancers are two of the most frequent cancers affecting women around the world, including Indonesia. Natural materials such as rodent tuber (Typhonium flagelliforme) have anticancer potentials. The rodent tuber extract contains ribosome inactivating proteins (RIPs) capable of cutting the DNA or RNA of cancer cells and blocking the growth of cancer cells. The purpose of this study was to evaluate the cytotoxic effects of Typhonium flagelliforme Lodd extract on HeLa cervical cancer and Michigan Cancer Foundation-7 (MCF-7) breast cancer cells. METHODS Subjects were cultured cell lines of HeLa cells in Rosswell Park Memorial Institute (RPMI) and of MCF-7 cells in Dulbecco’s Minimum Essential Medium (DMEM). Rodent tuber ethanolic extract was diluted in dimethyl sulfoxide (DMSO). The cytotoxicity assay used the 3-(4,5-dimethyl thiazol-2-yl,5-diphenyl) tetrazolium bromide (MTT) method. Absorbance was read in an ELISA reader at a wavelength of 595 nm. RESULTS Rat tuber extract at all dilutions (500; 250; 125; 62.5; 31.25; 15.625;7.81; 3.9 ì g/ mL) showed cytotoxic effects against HeLa and MCF-7 cells. Higher concentrations of the extract gave a higher proliferation inhibition effect. Calculated IC50 values of the extract by probit analysis were 30.19 ìg/mL against HeLa cells and 5.586 ì g/mL against MCF-7 cells. CONCLUSIONS Ethanolic extract of Typhonium flagelliforme Lodd has cytotoxic effects against HeLa cells and MCF-7 cells. The cytotoxic effects against MCF-7 cells are greater than the cytotoxic effects against HeLa cells

Examination of Telomerase Expression with Immuno-Hystochemistry Techniques on Some of Cancer Cells

Background: Cancer is a disease that gets serious attention in the medical world. This is due to the ever increasing number of patients and there has been no effective way to treat. Cancer cells have telomerase activity is relatively high compared to normal cells, so the cancer cells have the ability to continue to proliferate. Cancer cells undergo uncontrolled mitosis and have high telomerase activity compared to cells normal. Telomerase is an enzyme responsible for telomere length, a segment of DNA that is the tip of chromosomes in eukaryotic cells. Telomeres are associated with the process of ageing and carcinogenesis. The purpose of this study was to determine the expression of telomerase in some cells such as breast cancer, cervical cancer, and lung cancer. Methods: The research method is experimental studies in several cancer cell cultures in the form of cell line. Cancer cells used were: HeLa (cervical cancer), MCF7 and T47D (breast cancer), WiDr (lung cancer), and Raji (lymphoma) with culture medium RPMI, DMEM, and M199. Vero cells is used (fibroblast cells) as a control (normal cells). Expression of telomerase enzyme was measured by the Immunohystochemistry (IHC) method. Results: The results showed that the cancer cells have activity/higher telomerase expression were highly significant (p&lt;0.01) compared to normal cells (Vero cells). Similarly, the expression of telomerase in HeLa versus WiDr, WiDr versus T47D, T47D versus Raji, and Raji versus MCF7 also showed highly significant differences (p&lt;0.01). Telomerase expression between cancer cells that showed significant difference (HeLa cells versus Raji cells; HeLa cells versus MCF7 cell; T47D cells versus MCF7 cells) (p&lt;0.05). No significant difference was found in the group of HeLa cells versus T47D, WiDr versus Raji cells, and WiDr versus MCF7. Conclusions: It was concluded, that the cancer cells have telomerase expression of specific and different from each other, depending on the type of cell. T47D breast cancer cells have telomerase expression of the highest, followed by cervical cancer cells (HeLa). Lung cancer cells (WiDr) with cell lymphoma (Raji) has almost the same expression and both have lower expression