The vaginal-PVPA: A vaginal mucosa-mimicking in vitro permeation tool for evaluation of mucoadhesive formulations

Drug administration to the vaginal site has gained increasing attention in past decades, highlighting the need for reliable in vitro methods to assess the performance of novel formulations. To optimize formulations destined for the vaginal site, it is important to evaluate the drug retention within the vagina as well as its permeation across the mucosa, particularly in the presence of vaginal fluids. Herewith, the vaginal-PVPA (Phospholipid Vesicle-based Permeation Assay) in vitro permeability model was validated as a tool to evaluate the permeation of the anti-inflammatory drug ibuprofen from liposomal formulations (i.e., plain and chitosan-coated liposomes). Drug permeation was assessed in the presence and absence of mucus and simulated vaginal fluid (SVF) at pH conditions mimicking both the healthy vaginal premenopausal conditions and vaginal infection/pre-puberty/post-menopause state. The permeation of ibuprofen proved to depend on the type of formulation (i.e., chitosan-coated liposomes exhibited lower drug permeation), the mucoadhesive formulation properties and pH condition. This study highlights both the importance of mucus and SVF in the vaginal model to better understand and predict the in vivo performance of formulations destined for vaginal administration, and the suitability of the vaginal-PVPA model for such investigations

The standard of living in Latin America during the twentieth century

New and consistent series for Latin American real incomes, life expectancy and adult literacy over the twentieth century reveal that living standards rose most rapidly between the nineteen-thirties and -seventies, a period characterised by increased state intervention and reduced trade openness. Within the region, Brazil and Mexico advanced most over the century as a whole despite the early start made by Argentina and Chile, although convergence between larger countries was accompanied by divergence from smaller ones. There was no sustained narrowing of the income gap with the US at all between 1900 and 2000 but some convergence in living standards due to improved life expectancy. Our new estimates of regional per capita income also permit a clearer comparison with both Europe and Asia. The major advances in living standards achieved in the middle decades of the century were closely related to early industrialisation, rapid urbanisation and the extension of primary health and education. Subsequent economic volatility and fiscal fragility limited further increases in living standards, undermining social consensus on development strategy