Experimental characterization of the effects of pneumatic tubing on unsteady pressure measurements

Advances in aircraft control system designs have, with increasing frequency, required that air data be used as flight control feedback. This condition requires that these data be measured with accuracy and high fidelity. Most air data information is provided by pneumatic pressure measuring sensors. Typically unsteady pressure data provided by pneumatic sensing systems are distorted at high frequencies. The distortion is a result of the pressure being transmitted to the pressure sensor through a length of connective tubing. The pressure is distorted by frictional damping and wave reflection. As a result, air data provided all-flush, pneumatically sensed air data systems may not meet the frequency response requirements necessary for flight control augmentation. Both lab and flight test were performed at NASA-Ames to investigate the effects of this high frequency distortion in remotely located pressure measurement systems. Good qualitative agreement between lab and flight data are demonstrated. Results from these tests are used to describe the effects of pneumatic distortion in terms of a simple parametric model

Using H-alpha Morphology and Surface Brightness Fluctuations to Age-Date Star Clusters in M83

We use new WFC3 observations of the nearby grand design spiral galaxy M83 to develop two independent methods for estimating the ages of young star clusters. The first method uses the physical extent and morphology of Halpha emission to estimate the ages of clusters younger than tau ~10 Myr. It is based on the simple premise that the gas in very young (tau < few Myr) clusters is largely coincident with the cluster stars, is in a small, ring-like structure surrounding the stars in slightly older clusters (e.g., tau ~5 Myr), and is in a larger ring-like bubble for still older clusters (i.e., ~5-10 Myr). The second method is based on an observed relation between pixel-to-pixel flux variations within clusters and their ages. This method relies on the fact that the brightest individual stars in a cluster are most prominent at ages around 10 Myr, and fall below the detection limit (i.e., M_V < -3.5) for ages older than about 100 Myr. These two methods are the basis for a new morphological classification system which can be used to estimate the ages of star clusters based on their appearance. We compare previous age estimates of clusters in M83 determined from fitting UBVI Halpha measurements using predictions from stellar evolutionary models with our new morphological categories and find good agreement at the ~95% level. The scatter within categories is ~0.1 dex in log tau for young clusters (10 Myr) clusters. A by-product of this study is the identification of 22 "single-star" HII regions in M83, with central stars having ages ~4 Myr.Comment: 33 pages, 10 figures, 3 tables; published in March Ap

Hubble Space Telescope Images of Stephan's Quintet: Star Cluster Formation in a Compact Group Environment

Analysis of Hubble Space Telescope/Wide Field Planetary Camera 2 images of Stephan's Quintet, Hickson Compact Group 92, yielded 115 candidate star clusters (with V-I < 1.5). Unlike in merger remants, the cluster candidates in Stephan's Quintet are not clustered in the inner regions of the galaxies; they are spread over the debris and surrounding area. Specifically, these sources are located in the long sweeping tail and spiral arms of NGC 7319, in the tidal debris of NGC 7318B/A, and in the intragroup starburst region north of these galaxies. Analysis of the colors of the clusters indicates several distinct epochs of star formation that appear to trace the complex history of dynamical interactions in this compact group.Comment: 24 pages, 21 figures (13 PostScript and 8 JPEG), LaTeX (uses aastexug.sty), accepted for publication in the Astronomical Journal (July 2001). Full-resolution PostScript figures available at http://www.astro.psu.edu/users/gallsc/sq/figs.tar.g

Mannose binding lectin is required for alphavirus-induced arthritis/myositis

Mosquito-borne alphaviruses such as chikungunya virus and Ross River virus (RRV) are emerging pathogens capable of causing large-scale epidemics of virus-induced arthritis and myositis. The pathology of RRV-induced disease in both humans and mice is associated with induction of the host inflammatory response within the muscle and joints, and prior studies have demonstrated that the host complement system contributes to development of disease. In this study, we have used a mouse model of RRV-induced disease to identify and characterize which complement activation pathways mediate disease progression after infection, and we have identified the mannose binding lectin (MBL) pathway, but not the classical or alternative complement activation pathways, as essential for development of RRV-induced disease. MBL deposition was enhanced in RRV infected muscle tissue from wild type mice and RRV infected MBL deficient mice exhibited reduced disease, tissue damage, and complement deposition compared to wild-type mice. In contrast, mice deficient for key components of the classical or alternative complement activation pathways still developed severe RRV-induced disease. Further characterization of MBL deficient mice demonstrated that similar to C3(-/-) mice, viral replication and inflammatory cell recruitment were equivalent to wild type animals, suggesting that RRV-mediated induction of complement dependent immune pathology is largely MBL dependent. Consistent with these findings, human patients diagnosed with RRV disease had elevated serum MBL levels compared to healthy controls, and MBL levels in the serum and synovial fluid correlated with severity of disease. These findings demonstrate a role for MBL in promoting RRV-induced disease in both mice and humans and suggest that the MBL pathway of complement activation may be an effective target for therapeutic intervention for humans suffering from RRV-induced arthritis and myositis.This work was supported by NIH/NIAMS R01 AR 047190 awarded to MTH

Interaction of Oral Bacteria With Gingival Epithelial Cell Multilayers

Primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. Multilayers maintained at an air-liquid interface in low calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. Multilayers were infected with fluorescently labeled Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum or Streptococcus gordonii, and bacterial association was determined by confocal microscopy and quantitative image analysis. P. gingivalis invaded intracellularly and spread cell to cell. A. actinomycetemcomitans and F. nucleatum remained extracellular and showed intercellular movement through the multilayer. S. gordonii remained extracellular and predominantly associated with the superficial cell layer. None of the bacterial species disrupted barrier function as measured by transepithelial electrical resistance. P. gingivalis did not elicit secretion of proinflammatory cytokines. However, A. actinomycetemcomitans and S. gordonii induced IL-1β, TNF-α, IL-6 and IL-8 secretion; and F. nucleatum stimulated production of IL-1β and TNF-α. A. actinomycetemcomitans, F. nucleatum and S. gordonii, but not P. gingivalis, increased levels of apoptosis after 24 h infection. The results indicate that the organisms with pathogenic potential were able to traverse the epithelium, while the commensal bacteria did not. In addition, distinct host responses characterized the interaction between the junctional epithelium and oral bacteria

Ross River virus envelope glycans contribute to disease through activation of the host complement system

Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis. As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV envelope glycoproteins act as ligands for MBL. Using a panel of RRV mutants lacking the envelope N-linked glycans, we found that MBL deposition onto infected cells was dependent on the E2 glycans. Moreover, the glycan-deficient viruses exhibited reduced disease and tissue damage in a mouse model of RRV-induced myositis compared to wild-type RRV, despite similar viral load and inflammatory infiltrates within the skeletal muscle. Instead, the reduced disease induced by glycan-deficient viruses was linked to decreased MBL deposition and complement activation within inflamed tissues. These results demonstrate that the viral N-linked glycans promote MBL deposition and complement activation onto RRV-infected cells, contributing to the development of RRV-induced myositis

Toward Near‐Field Tsunami Forecasting Along the Cascadia Subduction Zone Using Rapid GNSS Source Models

Over the past 15 years and through multiple large and devastating earthquakes, tsunami warning systems have grown considerably in their efficacy in providing timely and accurate forecasts to affected communities. However, one part of tsunami warning that still needs improvement is forecasts catered to local, near‐field communities in the time after an earthquake rupture but before coastal inundation. In this study, we test a rapid, Global Navigation Satellite Systems (GNSS)‐driven earthquake characterization model using a large data set of synthetic megathrust ruptures for its near‐field tsunami forecasting potential. We also provide a framework for tsunami forecasting that focuses on the likelihood of exceedance of user‐defined coastal amplitudes that may be of use in the first 15 min following an earthquake. Specifically, we can estimate the earthquake magnitude, without saturation, for 82% of tested ruptures. We can also identify test ruptures as dominantly thrust events, without analyst guidance for 92% of tested thrust ruptures. Finally, modeling the tsunami component of our rapidly estimated fault rupture leads to greater than 80% accuracy in identifying tsunami impact at key coastal amplitude thresholds. This is promising for near‐field warning when the time prior to inundation is limited to tens of minutes. We focus this study on large megathrust ruptures along the quiescent Cascadia subduction zone where there is already a dense GNSS network

Improved KL->pi e nu Form Factor and Phase Space Integral with Reduced Model Uncertainty

Using the published KTeV sample of 2 million KL-> pi e nu decays and a new form factor expansion with a rigorous bound on higher order terms, we present a new determination of the KL->pi e nu form factor and phase space integral. Compared to the previous KTeV result, the uncertainty in the new form factor expansion is negligible and results in an overall uncertainty in the phase space integral (IKe) that is a factor of two smaller: IKe = 0.15392 +- 0.00048 \.Comment: 3 pages, 2 figures, submitted to PRD Rapid Communicatio

Measurements of the Decay KL→e+e−γK_L \to e^+e^-\gamma

The E799-II (KTeV) experiment at Fermilab has collected 83262 $K_L \to e^+e^-\gamma$ events above a background of 79 events. We measure a decay width, normalized to the $K_L \to \pi^0\pi^0\pi^0_D$ (\pi^0 \to \gamma\gamma, \pi^0 to \gamma\gamma, \pi^0_D \to e^+e^-\gamma) decay width, of $\Gamma($K_L \to e^+e^-\gamma$)/\Gamma(K_L \to \pi^0\pi^0\pi^0_D) = (1.3302 \pm 0.0046_{stat} \pm 0.0102_{syst}) \times 10^{-3}$. We also measure parameters of two $K_L \gamma^{\ast}\gamma$ form factor models. In the Bergstrom, Masso, and Singer (BMS) parametrization, we find \caks = -0.517 \pm 0.030_{stat} \pm 0.022_{syst}. We separately fit for the first parameter of the D'Ambrosio, Isidori, and Portoles (DIP) model and find \adip = -1.729 \pm 0.043_{stat} \pm 0.028_{syst}.Comment: 5 pages, 3 figures, submitted to PR