Olfactory variation in mouse husbandry and its implications for refinement and standardisation: UK survey of non-animal scents

With their highly sensitive olfactory system, the behaviour and physiology of mice are not only influenced by the scents of conspecifics and other species, but also by many other chemicals in the environment. The constraints of laboratory housing limit a mouse’s capacity to avoid aversive odours that could be present in the environment. Potentially odorous items routinely used for husbandry procedures, such as sanitizing products and gloves, could be perceived by mice as aversive or attractive, and affect their behaviour, physiology and experimental results. A survey was sent to research institutions in the UK to enquire about husbandry practices that could impact on the olfactory environment of the mouse. Responses were obtained from 80 individuals working in 51 institutions. Husbandry practices varied considerably. Seventy percent of respondents reported always wearing gloves for handling mice, with nitrile being the most common glove material (94%) followed by latex (23%) and vinyl (14%). Over six different products were listed for cleaning surfaces, floors, anaesthesia and euthanasia chambers and behavioural apparatus. In all cases Trigene™ (now called Anistel™) was the most common cleaning product used (43, 41, 40 and 49%, respectively). Depending on the attribute considered, between 7 and 19% of respondents thought that cleaning products definitely, or were likely to, have strong effects on standardization, mouse health, physiology or behaviour. Understanding whether and how these odours affect mouse welfare will help to refine mouse husbandry and experimental procedures through practical recommendations, to improve the quality of life of laboratory animals and the experimental data obtained

Bone sequestrum in a yearling red deer (Cervus elaphus) hind in New Zealand.

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A Collective Route to Head and Neck Cancer Metastasis

© 2018 The Author(s). Distant metastasis (DM) from head and neck cancers (HNC) portends a poor patient prognosis. Despite its important biological role, little is known about the cells which seed these DM. Circulating tumour cells (CTCs) represent a transient cancer cell population, which circulate in HNC patients' peripheral blood and seed at distant sites. Capture and analysis of CTCs offers insights into tumour metastasis and can facilitate treatment strategies. Whilst the data on singular CTCs have shown clinical significance, the role of CTC clusters in metastasis remains limited. In this pilot study, we assessed 60 treatment naïve HNC patients for CTCs with disease ranging from early to advanced stages, for CTC clusters utilizing spiral CTC enrichment technology. Single CTCs were isolated in 18/60-30% (Ranging from Stage I-IV), CTC clusters in 15/60-25% (exclusively Stage IV) with 3/15-20% of CTC clusters also containing leukocytes. The presence of CTC clusters associated with the development of distant metastatic disease(P = 0.0313). This study demonstrates that CTC clusters are found in locally advanced patients, and this may be an important prognostic marker. In vivo and in vitro studies are warranted to determine the role of these CTC clusters, in particular, whether leukocyte involvement in CTC clusters has clinical relevance

Equity of people with dementia in research, why does this issue remain?

Research in dementia has moved beyond the question of how, why and should people with dementia be participants, to the question of how to collaborate with people with dementia in the development, design, implementation and dissemination of research in dementia. So, why are we still discussing the exclusion of people with dementia in research and the need to develop human rights‐based frameworks to address issues of consent, capacity and inclusion

Exploration of crime-scene characteristics in juvenile homicide in the French-speaking part of Belgium

This study explores modeling crime-scene characteristics of juvenile homicide in the French-speaking part of Belgium. Multidimensional scaling analysis was carried out on crime-scene characteristics derived from the court files of 67 individuals under 22 years old, who had been charged with murder or attempted murder (1995-2009). Three thematic regions (Expressive: multiple offenders; Instrumental: theft; Instrumental: sex/forensic awareness) distinguished types of aggression displayed during the offense. These themes reaffirm that the expressive-instrumental differentiation found in general homicide studies is valuable when attempting to discriminate juvenile homicides. The proposed framework was found useful to classify the offenses, as 84% of homicides were assigned to a dominant theme. Additionally, associations between crime-scene characteristics and offenders' characteristics were analyzed, but no associations were found, therefore failing to provide empirical support for the homology assumption. Cultural comparisons, as well as the influence of age on the thematic structure are discussed

Biology and ecology of the invasive lionfishes, Pterois miles and Pterois volitans

The Indo-Pacific lionfishes, Pterois miles and P. volitans, are now established along the U.S. southeast coast, Bermuda, Bahamas, and are becoming established in the Caribbean. While these lionfish are popular in the aquarium trade, their biology and ecology are poorly understood in their native range. Given the rapid establishment and potential adverse impacts of these invaders, comprehensive studies of their biology and ecology are warranted. Here we provide a synopsis of lionfish biology and ecology including invasion chronology, taxonomy, local abundance, reproduction, early life history and dispersal, venomology, feeding ecology, parasitology, potential impacts, and control and management. This information was collected through review of the primary literature and published reports and by summarizing current observations. Suggestions for future research on invasive lionfish in their invaded regions are provided