Pedagogies of E-literary Practice for (Un)continuous Times: Lightning Talks

In response to Debbie Chacra’s charge to “celebrate and foster education, maintenance, analysis, critique, and, above all, caregiving” in maker cultures, this roundtable imagines pedagogies of e-literary practice that combine creation and caregiving in ways restorative to the ongoing tradition(s) of e-lit (“Beyond Making”). E-lit regularly confronts theoretical, cultural, and material challenges endemic to the field, as genres of previously accessible work are being lost to technological obsolescence and new developments are moving increasingly off the screen and out of practical reach. One way to counter such challenges of these (un)continuous times is through an integrated, applied, practice-based model of e-lit pedagogy. Participants discuss ways of teaching electronic literature that incorporates hands-on work like critical-creative making and procedural performance as pedagogical responses to rapid technological changes that blot out e-literature’s past and blackbox its future

Common genetic variants in the CLDN2 and PRSS1-PRSS2 loci alter risk for alcohol-related and sporadic pancreatitis

Pancreatitis is a complex, progressively destructive inflammatory disorder. Alcohol was long thought to be the primary causative agent, but genetic contributions have been of interest since the discovery that rare PRSS1, CFTR, and SPINK1 variants were associated with pancreatitis risk. We now report two significant genome-wide associations identified and replicated at PRSS1-PRSS2 (1×10-12) and x-linked CLDN2 (p < 1×10-21) through a two-stage genome-wide study (Stage 1, 676 cases and 4507 controls; Stage 2, 910 cases and 4170 controls). The PRSS1 variant affects susceptibility by altering expression of the primary trypsinogen gene. The CLDN2 risk allele is associated with atypical localization of claudin-2 in pancreatic acinar cells. The homozygous (or hemizygous male) CLDN2 genotype confers the greatest risk, and its alleles interact with alcohol consumption to amplify risk. These results could partially explain the high frequency of alcohol-related pancreatitis in men – male hemizygous frequency is 0.26, female homozygote is 0.07

Comprehensive Use Case Scenario Generation: An Approach for Modeling System of Systems Behaviors

This paper describes a reusable approach using automatic use case scenario generation to specify system of systems (SoS) behaviors. The approach leverages the Monterey Phoenix (MP) language and tools, which extend current methods significantly by making a larger number of SoS use case scenario variants available to SoS analysts. Having described how MP structures behavior models to accomplish this task in previous papers, this paper provides model-building details and advice that takes readers through the approach so they may repeat it on a SoS of their own interest. A simplified SoS model of three systems interacting in a humanitarian assistance and disaster relief (HADR) scenario is used to demonstrate the approach. The HADR example highlights the model structure features that enable automatic SoS use case scenario generation, and supporting descriptions of each part of the model are provided. The paper concludes with a summary and brief discussion of ongoing research areas that support bringing this approach into the mainstream of system of systems engineering practice.This work was sponsored by the Naval Air System Command (NAVAIR) under Systems Engineering Transformation and is a product of the Systems Engineering Research Center (SERC) research task RT-176.This work was sponsored by the Naval Air System Command (NAVAIR) under Systems Engineering Transformation and is a product of the Systems Engineering Research Center (SERC) research task RT-176

Pedagogies of E-literary Practice for (Un)continuous Times: Live Recording

This is the full recording of the live panel: for the lightning talks, please see the other entry. In response to Debbie Chacra’s charge to “celebrate and foster education, maintenance, analysis, critique, and, above all, caregiving” in maker cultures, this roundtable imagines pedagogies of e-literary practice that combine creation and caregiving in ways restorative to the ongoing tradition(s) of e-lit (“Beyond Making”). E-lit regularly confronts theoretical, cultural, and material challenges endemic to the field, as genres of previously accessible work are being lost to technological obsolescence and new developments are moving increasingly off the screen and out of practical reach. One way to counter such challenges of these (un)continuous times is through an integrated, applied, practice-based model of e-lit pedagogy. Participants discuss ways of teaching electronic literature that incorporates hands-on work like critical-creative making and procedural performance as pedagogical responses to rapid technological changes that blot out e-literature’s past and blackbox its future

Mass-spectrometry-based quantitation of Her2 in gastroesophageal tumor tissue: comparison to IHC and FISH

Trastuzumab has shown a survival benefit in cases of Her2-positive gastroesophageal cancer (GEC). Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) currently determine eligibility for trastuzumab-based therapy. However, these low-throughput assays often produce discordant or equivocal results. We developed a targeted proteomic assay based on selected reaction monitoring mass spectrometry (SRM-MS) and quantified levels (amol/mu g) of Her2-SRM protein in cell lines (n = 27) and GEC tissues (n = 139). We compared Her2-SRM protein expression with IHC/FISH, seeking to determine optimal SRM protein expression cutoffs in order to identify HER2 gene amplification. After demonstrating assay development, precision, and stability, Her2-SRM protein measurement was observed to be highly concordant with the HER2/CEP17 ratio, particularly in a multivariate regression model adjusted for SRM expression of the covariates Met, Egfr, Her3, and HER2 heterogeneity, as well as their interactions (cell lines r (2) = 0.9842; FFPE r (2) = 0.7643). In GEC tissues, Her2-SRM protein was detected at any level in 71.2 % of cases. ROC curves demonstrated that Her2-SRM protein levels have a high specificity (100 %) at an upper-level cutoff of > 750 amol/A mu g and sensitivity of 75 % at a lower-level cutoff of < 450 amol/mu g for identifying HER2 FISH-amplified tumors. An "equivocal zone" of 450-750 amol/A mu g of Her2-SRM protein was analogous to IHC2+ but represented fewer cases (9-16 % of cases versus 36-41 %). Compared to IHC, targeted SRM-Her2 proteomics provided more objective and quantitative Her2 expression with excellent HER2/CEP17 FISH correlation and fewer equivocal cases. Along with its multiplex capability for other relevant oncoproteins, these results demonstrate a refined HER2 protein expression assay for clinical application