Characterization of Clinically-Attenuated Burkholderia mallei by Whole Genome Sequencing: Candidate Strain for Exclusion from Select Agent Lists

is an understudied biothreat agent responsible for glanders which can be lethal in humans and animals. Research with this pathogen has been hampered in part by constraints of Select Agent regulations for safety reasons. Whole genomic sequencing (WGS) is an apt approach to characterize newly discovered or poorly understood microbial pathogens. genome. Therefore, the strain by itself is unlikely to revert naturally to its virulent phenotype. There were other genes present in one strain and not the other and vice-versa. was both avirulent in the natural host ponies, and did not possess T3SS associated genes may be fortuitous to advance biodefense research. The deleted virulence-essential T3SS is not likely to be re-acquired naturally. These findings may provide a basis for exclusion of SAVP1 from the Select Agent regulation or at least discussion of what else would be required for exclusion. This exclusion could accelerate research by investigators not possessing BSL-3 facilities and facilitate the production of reagents such as antibodies without the restraints of Select Agent regulation

Deadly Cultures: Biological Weapons Since 1945.

NoThe threat of biological weapons has never attracted as much public attention as in the past five years. Current concerns largely relate to the threat of weapons acquisition and use by rogue states or by terrorists. But the threat has deeper roots--it has been evident for fifty years that biological agents could be used to cause mass casualties and large-scale economic damage. Yet there has been little historical analysis of such weapons over the past half-century. Deadly Cultures sets out to fill this gap by analyzing the historical developments since 1945 and addressing three central issues: Why have states continued or begun programs for acquiring biological weapons? Why have states terminated biological weapons programs? How have states demonstrated that they have truly terminated their biological weapons programs? We now live in a world in which the basic knowledge needed to develop biological weapons is more widely available than ever before. Deadly Cultures provides the lessons from history that we urgently need in order to strengthen the long-standing prohibition of biological weapons