1,373 research outputs found

    The phase coherence of light from extragalactic sources - direct evidence against first order Planck scale fluctuations in time and space

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    We present a method of directly testing whether time continues to have its usual meaning on scales of <= t_P = sqrt(hbar G/c^5) ~ 5.4E-44 s, the Planck time. According to quantum gravity, the time t of an event cannot be determined more accurately than a standard deviation of the form sigma_t/t = a_o (t_P/t)^a, where a_o and a are positive constants ~1; likewise distances are subject to an ultimate uncertainty c \sigma_t, where c is the speed of light. As a consequence, the period and wavelength of light cannot be specified precisely; rather, they are independently subject to the same intrinsic limitations in our knowledge of time and space, so that even the most monochromatic plane wave must in reality be a superposition of waves with varying omega and {\bf k}, each having a different phase velcocity omega/k. For the entire accessible range of the electromagnetic spectrum this effect is extremely small, but can cumulatively lead to a complete loss of phase information if the emitted radiation propagated a sufficiently large distance. Since, at optical frequencies, the phase coherence of light from a distant point source is a necessary condition for the presence of diffraction patterns when the source is viewed through a telescope, such observations offer by far the most sensitive and uncontroversial test. We show that the HST detection of Airy rings from the active galaxy PKS1413+135, located at a distance of 1.2 Gpc, secures the exclusion of all first order (a=1) quantum gravity fluctuations with an amplitude a_o > 0.003. The same result may be used to deduce that the speed of light in vacuo is exact to a few parts in 10^32.Comment: Title change. One reference added. Final version accepted by ApJ

    Automated identification of flagella from videomicroscopy via the medial axis transform

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    Ubiquitous in eukaryotic organisms, the flagellum is a well-studied organelle that is well-known to be responsible for motility in a variety of organisms. Commonly necessitated in their study is the capability to image and subsequently track the movement of one or more flagella using videomicroscopy, requiring digital isolation and location of the flagellum within a sequence of frames. Such a process in general currently requires some researcher input, providing some manual estimate or reliance on an experiment-specific heuristic to correctly identify and track the motion of a flagellum. Here we present a fully-automated method of flagellum identification from videomicroscopy based on the fact that the flagella are of approximately constant width when viewed by microscopy. We demonstrate the effectiveness of the algorithm by application to captured videomicroscopy of Leishmania mexicana, a parasitic monoflagellate of the family Trypanosomatidae. ImageJ Macros for flagellar identification are provided, and high accuracy and remarkable throughput are achieved via this unsupervised method, obtaining results comparable in quality to previous studies of closely-related species but achieved without the need for precursory measurements or the development of a specialised heuristic, enabling in general the automated generation of digitised kinematic descriptions of flagellar beating from videomicroscopy.Comment: 10 pages, 5 figures. Author accepted manuscript. Supplementary Material available at https://doi.org/10.1038/s41598-019-41459-

    Boundary behaviours of Leishmania mexicana: a hydrodynamic simulation study

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    It is well established that the parasites of the genus Leishmania exhibit complex surface interactions with the sandfly vector midgut epithelium, but no prior study has considered the details of their hydrodynamics. Here, the boundary behaviours of motile Leishmania mexicana promastigotes are explored in a computational study using the boundary element method, with a model flagellar beating pattern that has been identified from digital videomicroscopy. In particular a simple flagellar kinematics is observed and quantified using image processing and mode identification techniques, suggesting a simple mechanical driver for the Leishmania beat. Phase plane analysis and long-time simulation of a range of Leishmania swimming scenarios demonstrate an absence of stable boundary motility for an idealised model promastigote near passive or repulsive surfaces, with behaviours ranging from boundary capture to deflection into the bulk. Indeed, the inclusion of a repulsive surface force results in the deflection of all surface-bound promastigotes, suggesting that the documented surface detachment of infective metacyclic promastigotes may be the result of morphological adaptation and simple hydrodynamics. Further, simulation elucidates a remarkable morphology-dependent hydrodynamic mechanism of boundary approach, hypothesised to be the cause of the well-established phenomenon of tip-first epithelial attachment of Leishmania promastigotes to the sandfly vector midgut.Comment: 12 pages, 9 figures. Supplementary Material available upon reques

    Theology, News and Notes - Vol. 50, No. 01

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    Theology News & Notes was a theological journal published by Fuller Theological Seminary from 1954 through 2014.https://digitalcommons.fuller.edu/tnn/1146/thumbnail.jp

    Structural and wetting properties of nature\u27s finest silks (order Embioptera)

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    Insects from the order Embioptera (webspinners) spin silk fibres which are less than 200 nm in diameter. In this work, we characterized and compared the diameters of single silk fibres from nine species—Antipaluria urichi, Pararhagadochir trinitatis, Saussurembia calypso, Diradius vandykei, Aposthonia ceylonica, Haploembia solieri, H. tarsalis, Oligotoma nigra and O. saundersii. Silk from seven of these species have not been previously quantified. Our studies cover five of the 10 named taxonomic families and represent about one third of the known taxonomic family-level diversity in the order Embioptera. Naturally spun silk varied in diameter from 43.6 ± 1.7 nm for D. vandykei to 122.4 ± 3.2 nm for An. urichi. Mean fibre diameter did not correlate with adult female body length. Fibre diameter is more similar in closely related species than in more distantly related species. Field observations indicated that silk appears shiny and smooth when exposed to rainwater. We therefore measured contact angles to learn more about interactions between silk and water. Higher contact angles were measured for silks with wider fibre diameter and higher quantity of hydrophobic amino acids. High static contact angles (ranging up to 122° ± 3° for An. urichi) indicated that silken sheets spun by four arboreal, webspinner species were hydrophobic. A second contact angle measurement made on a previously wetted patch of silk resulted in a lower contact angle (average difference was greater than 27°) for all four species. Our studies suggest that silk fibres which had been previously exposed to water exhibited irreversible changes in hydrophobicity and water adhesion properties. Our results are in alignment with the ‘super-pinning’ site hypothesis by Yarger and co-workers to describe the hydrophobic, yet water adhesive, properties exhibited by webspinner silk fibres. The physical and chemical insights gained here may inform the synthesis and development of smaller diameter silk fibres with unique water adhesion properties

    Ammonia Emissions from Twelve U.S. Broiler Chicken Houses

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    Twelve commercial broiler houses in the U.S. were each monitored for at least thirteen 48 h periods over the course of one year to obtain ammonia emission data. Paired repetition of houses on four farms represents current construction with variety in litter management (built-up or new litter each flock) and climate conditions (cold or mixed-humid). Ammonia concentration was determined using portable electrochemical sensors incorporating a fresh air purge cycle. Ventilation rate was determined via in-situ measurement of fan capacity, fan on-off times, and house static pressure difference. There were seasonal trends in exhaust ammonia concentration (highest in cold weather) and ventilation rates (highest in warm weather) but not for emission rate. Flocks with at least three monitoring periods (13 of 22 flocks) demonstrated similar emission rates at a given bird age among the four study farms and across the seasons. An analysis of emissions from all houses on the three farms using built-up litter resulted in predicted regression slopes of 0.028, 0.034, and 0.038 g NH3 bird-1 d-1 per day of age; the fourth farm, managed with new litter, had the lowest emission rate at 0.024 g NH3 bird-1 d-1. The intercept of these composite relationships was influenced by litter conditions, with flocks on new litter having essentially no emissions for about six days while built-up litter flocks had emissions starting at flock placement. Data from all four farms and all flocks provided a regression slope of 0.031(±0.001 std error) g NH3 bird-1 d-1 per day of age. Emission rate per animal unit for built-up litter flocks indicated very high emissions for the youngest birds (under 14 days of age), after which time the emissions decreased exponentially and were then relatively steady for the balance of the flock cycle
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