The Lantern Vol. 17, No. 2, Spring 1949

• Psyche • Home Country • Liberation • The Last Haul • The Tempting of Willie • The Turtle • Interlude • Black Waters • Lines on Abandoned Spring House • Afraid • Gone is the Winter\u27s Night • A Word to the Wisehttps://digitalcommons.ursinus.edu/lantern/1047/thumbnail.jp

The Lantern Vol. 17, No. 3, Summer 1949

• All the Silver in Taxco • The Fall • Parlor Games • Something There Is • Friday Night • Evening • Checker-Board Country • A Noise • Expected Up In Heaven Today • When Time Has Torn My Youth • Impression of Deathhttps://digitalcommons.ursinus.edu/lantern/1048/thumbnail.jp

The Lantern Vol. 16, No. 3, Spring 1948

• Not So Light • Babba\u27s Luck • Winter Night • God Hath Wrought • Less Than Trivia • What is Progress? • Betrayal • The Key • Journey From a Star • War and Peace • Experiment in Prose Poetry • Dawn • Eternal Question • My Gift • Jazz Fantasy • M.W. Witmerhttps://digitalcommons.ursinus.edu/lantern/1045/thumbnail.jp

The Determination of alpha_s from Tau Decays Revisited

We revisit the determination of alpha_s(m_tau) using a fit to inclusive tau hadronic spectral moments in light of (1) the recent calculation of the fourth-order perturbative coefficient K_4 in the expansion of the Adler function, (2) new precision measurements from BABAR of e+e- annihilation cross sections, which decrease the uncertainty in the separation of vector and axial-vector spectral functions, and (3) improved results from BABAR and Belle on tau branching fractions involving kaons. We estimate that the fourth-order perturbative prediction reduces the theoretical uncertainty, introduced by the truncation of the series, by 20% with respect to earlier determinations. We discuss to some detail the perturbative prediction and show that the effect of the incomplete knowledge of the series is reduced by using the so-called contour-improved calculation, as opposed to fixed-order perturbation theory which manifests convergence problems. The corresponding theoretical uncertainties are studied at the tau and Z mass scales. Nonperturbative contributions extracted from the most inclusive fit are small, in agreement with earlier determinations. Systematic effects from quark-hadron duality violation are estimated with simple models and found to be within the quoted systematic errors. The fit gives alpha_s(m_tau) = 0.344 +- 0.005 +- 0.007, where the first error is experimental and the second theoretical. After evolution to M_Z we obtain alpha_s(M_Z) = 0.1212 +- 0.0005 +- 0.0008 +- 0.0005, where the errors are respectively experimental, theoretical and due to the evolution. The result is in agreement with the corresponding NNNLO value derived from essentially the Z width in the global electroweak fit. The alpha_s(M_Z) determination from tau decays is the most precise one to date.Comment: 22 pages, 7 figure

Identification of New Agonists and Antagonists of the Insect Odorant Receptor Co-Receptor Subunit

BACKGROUND: Insects detect attractive and aversive chemicals using several families of chemosensory receptors, including the OR family of olfactory receptors, making these receptors appealing targets for the control of insects. Insect ORs are odorant-gated ion channels, comprised of at least one common subunit (the odorant receptor co-receptor subunit, Orco) and at least one variable odorant specificity subunit. Each of the many ORs of an insect species is activated or inhibited by an unique set of odorants that interact with the variable odorant specificity subunits, making the development of OR directed insect control agents complex and laborious. However, several N-,2-substituted triazolothioacetamide compounds (VUAA1, VU0450667 and VU0183254) were recently shown to act directly on the highly conserved Orco subunit, suggesting that broadly active compounds can be developed. We have explored the chemical space around the VUAA1 structure in order to identify new Orco ligands. PRINCIPAL FINDINGS: We screened ORs from several insect species, using heterologous expression in Xenopus oocytes and an electrophysiological assay, with a panel of 22 compounds structurally related to VUAA1. By varying the nitrogen position in the pyridine ring and altering the moieties decorating the phenyl ring, we identified two new agonists and a series of competitive antagonists. Screening smaller compounds, similar to portions of the VUAA1 structure, also yielded competitive antagonists. Importantly, we show that Orco antagonists inhibit odorant activation of ORs from several insect species. Detailed examination of one antagonist demonstrated inhibition to be through a non-competitive mechanism. CONCLUSIONS: A similar pattern of agonist and antagonist sensitivity displayed by Orco subunits from different species suggests a highly conserved binding site structure. The susceptibility to inhibition of odorant activation by Orco antagonism is conserved across disparate insect species, suggesing the ligand binding site on Orco as a promising target for the development of novel, broadly active insect repellants

Triosephosphate isomerase I170V alters catalytic site, enhances stability and induces pathology in a Drosophila model of TPI deficiency ☆

Triosephosphate isomerase (TPI) is a glycolytic enzyme which homodimerizes for full catalytic activity. Mutations of the TPI gene elicit a disease known as TPI Deficiency, a glycolytic enzymopathy noted for its unique severity of neurological symptoms. Evidence suggests that TPI Deficiency pathogenesis may be due to conformational changes of the protein, likely affecting dimerization and protein stability. In this report, we genetically and physically characterize a human disease-associated TPI mutation caused by an I170V substitution. Human TPI I170V elicits behavioral abnormalities in Drosophila. An examination of hTPI I170V enzyme kinetics revealed this substitution reduced catalytic turnover, while assessments of thermal stability demonstrated an increase in enzyme stability. The crystal structure of the homodimeric I170V mutant reveals changes in the geometry of critical residues within the catalytic pocket. Collectively these data reveal new observations of the structural and kinetic determinants of TPI Deficiency pathology, providing new insights into disease pathogenesis