Control strains of <i>E</i>. <i>coli</i> using virulent specific primers.

<p>Lane M: Generuler 1 kb plus (Fermentas); lane C: Multiplex of e<i>ae</i> (EPEC), <i>ipaH</i> (EIEC), <i>stx</i> (EHEC), <i>aggR</i> (EAEC); lane 1: <i>eae</i> (881 bp); lane 2: <i>ipaH</i> (619 bp); lane 3: <i>stx</i> (518 bp); lane 4: <i>aggR</i> (254 bp) and lane N: negative control.</p

In vitro toxicity studies of novel solar water disinfection reactors using the E-screen bioassay and the Ames test

Abstract Solar water disinfection (SODIS) is a cost-effective point of use method for disinfecting water, usually in a 2 L polyethylene terephthalate (PET) plastic bottle. To increase the volume of water disinfected, three novel transparent reactors were developed using PET in 25 L transparent jerrycans, polymethyl methacrylate (PMMA) in tubular solar reactors capable of delivering >20 L of water and polypropylene (PP) in 20 L buckets. In vitro bioassays were used to investigate any toxic substances leached from the plastic reactors into disinfected water as a result of exposure to sunshine for up to 9 months. The Ames test was used to test for mutagenicity and the E-screen bioassay to test for estrogenicity. No mutagenicity was detected in any sample and no estrogenicity was found in the SODIS treated water produced by the PMMA reactors or the PP buckets. While water disinfected using the PET reactors showed no estrogenicity following exposure to the sun for 3 and 6 months, estrogenicity was detected following 9 months' exposure to sunlight; however levels detected were within the acceptable daily intake for 17β-estradiol (E2) of up to 50 ng/kg body weight/day