Requirement of endogenous tumor necrosis factor/cachectin for recovery from experimental peritonitis

By intrasplenic immunization we raised a rat mAb (mAb V1q; IgG2a, kappa) with a potent neutralizing activity against natural mouse TNF (1 microgram/ml mAb V1q/100 U/ml TNF). mAb V1q was used to study the role of endogenous TNF in experimental peritonitis induced by sublethal cecal ligation and puncture. mAb V1q persisted for over 5 days in the serum of mice injected with 100 micrograms of the antibody and, therefore, proved useful for in vivo experiments. As little as 20 micrograms mAb V1q/mouse prevented lethal shock of the animals by 400 micrograms LPS/mouse. In sublethal cecal ligation and puncture i.p. injection of mAb V1q directly and up to 8 h after induction of experimental peritonitis lead to death of the animals within 1 to 3 days. The lethal effect of mAb V1q was compensated by injection of recombinant mouse TNF. Similar mAb V1q effects as in immunocompetent mice were shown in severe combined immune deficiency mice deficient of mature functional B and T cells. Taken together, these data suggest that during the early phase of peritonitis endogenous TNF may stimulate nonlymphoid cells such as granulocytes, macrophages, platelets, and fibroblasts to ingest bacteria and to localize inflammation, respectively. These beneficial effects of TNF may determine survival. Thus, our data may have implications for the therapeutic management of a beginning peritonitis

Summary: Remote sensing soil moisture research

During the 1969 and 1970 growing seasons research was conducted to investigate the relationship between remote sensing imagery and soil moisture. The research was accomplished under two completely different conditions: (1) cultivated cropland in east central South Dakota, and (2) rangeland in western South Dakota. Aerial and ground truth data are being studied and correlated in order to evaluate the moisture supply and water use. Results show that remote sensing is a feasible method for monitoring soil moisture

Far-infrared line observations of planetary nebulae. 1: The O 3 spectrum

Observations of the far-infrared fine structure lines of O III have been obtained for six planetary nebulae. The infrared measurements are combined with optical O III line fluxes to probe physical conditions in the gas. From the observed line intensity ratios, a simultaneous solution was obtained for electron temperature and density, as well as means of evaluating the importance of inhomogeneities. Densities determined from the far-infrared O III lines agree well density diagnostics from other ions, indicating a fairly homogeneous density in the emitting gas. Temperatures are determined separately from the O III 4363/5007 A and 5007 A/52 micron intensity ratios and compared. Systematically higher values are derived from the former ratio, which is expected from a nebula which is not isothermal. Allowance for the presence of temperature variations within these nebulae raises their derived oxygen abundances, determinations to be reconciled with the solar value

Localization of fermions to branes: codimension d ≥ 2

Motivated by recent experiments, we consider a Schrödinger cat superposition of two widely separated coherent states in thermal equilibrium. The time development of our system is obtained using Wigner distribution functions. In contrast to our discussion for a two-Gaussian wave packet [Phys. Lett. A 286 (2001) 87], we find that, in the absence of dissipation, the interference term does not decay rapidly in time, but in common with the other two terms, it oscillates in time and persists for all times

The QSO evolution derived from the HBQS and other complete QSO surveys

An ESO Key programme dedicated to an Homogeneous Bright QSO Survey (HBQS) has been completed. 327 QSOs (Mb<-23, 0.3<z<2.2) have been selected over 555 deg^2 with 15<B<18.75. For B<16.4 the QSO surface density turns out to be a factor 2.2 higher than what measured by the PG survey, corresponding to a surface density of 0.013+/-.006 deg^{-2}. If the Edinburgh QSO Survey is included, an overdensity of a factor 2.5 is observed, corresponding to a density of 0.016+/-0.005 deg^{-2}. In order to derive the QSO optical luminosity function (LF) we used Monte Carlo simulations that take into account of the selection criteria, photometric errors and QSO spectral slope distribution. The LF can be represented with a Pure Luminosity Evolution (L(z)\propto(1+z)^k) of a two power law both for q_0=0.5 and q_0=0.1. For q_0=0.5 k=3.26, slower than the previous Boyle's (1992) estimations of k=3.45. A flatter slope beta=-3.72 of the bright part of the LF is also required. The observed overdensity of bright QSOs is concentrated at z<0.6. It results that in the range 0.3<z<0.6 the luminosity function is flatter than observed at higher redshifts. In this redshift range, for Mb<-25, 32 QSOs are observed instead of 19 expected from our best-fit PLE model. This feature requires a luminosity dependent luminosity evolution in order to satisfactorily represent the data in the whole 0.3<z<2.2 interval.Comment: Invited talk in "Wide Field Spectroscopy" (20-24 May 1996, Athens), eds. M. Kontizas et al. 6 pages and 3 eps figures, LaTex file, uses epfs.sty and crckapb.sty (included

A far-infrared study of N/O abundance ratio in galactic H 2 regions

Far-infrared lines of N++ and O++ in several galactic H II regions were measured in an effort to probe the abundance ratio N/O. New measurements are presented for W32 (630.8-0.0), Orion A, and G75.84+0.4. The combination of (N III) 57.3 millimicrons and (O III) 88.4 and 51.8 millimicrons yields measurements of N++/O++ that are largely insensitive to electron temperature, density uncertainties, and to clumping of the ionized gas, due to the similarity of the critical densities for these transitions. In the observed nebulae, N++/O++ should be indicative of N/O, a ratio that is of special importance in nucleosynthesis theory. Measurements are compared with previous measurements of M17 and W51. For nebulae in the solar circle, N++/O++ is greater than the N/O values derived from optical studies of N+/O+ in low ionization zones of the same nebulae. We find that N++/O++ in W43 is significantly higher than for the other H II regions in the sample. Since W43 is located at R = 5 kpc, which is the smallest galactocentric distance in our sample, our data appear consistent with the presence of a negative abundance gradient d(N/O)dR

Rapid enzymatic test for phenotypic HIV protease drug resistance

A phenotypic resistance test based on recombinant expression of the active HIV protease in E. coli from patient blood samples was developed. The protease is purified in a rapid onestep procedure as active enzyme and tested for inhibition by five selected synthetic inhibitors (amprenavir, indinavir, nelfinavir, ritonavir, and saquinavir) used presently for chemotherapy of HIVinfected patients. The HPLC system used in a previous approach was replaced by a continuous fluorogenic assay suitable for highthroughput screening on microtiter plates. This reduces significantly the total assay time and allows the determination of inhibition constants (K-i). The Michaelis constant (K-m) and the inhibition constant (K-i) of recombinant wildtype protease agree well with published data for cloned HIV protease. The enzymatic test was evaluated with recombinant HIV protease derived from eight HIVpositive patients scored from sensitive to highly resistant according to mutations detected by genotypic analysis. The measured K-i values correlate well with the genotypic resistance scores, but allow a higher degree of differentiation. The noninfectious assay enables a more rapid yet sensitive detection of HIV protease resistance than other phenotypic assays