5 research outputs found
Nano-calciumphosphate generation with novel surface and chemical features for improvement of cell activity in bone repair and replacement
Ideal biomaterial for bone replacement implanted should be resorbed by osteoclasts, while osteoblastic activity deposits new bone. Electrospun biphasic nanobioceramic scaffolds were synthesized for in vitro testing,
contributing to bone tissue engineering.This paper was initially delivered at the Annual Congress of the Biological
Sciences Division of the South African Academy for Science and Art, ARC-Plant Protection Research Institute, Roodeplaat, Pretoria, South
Africa on 01 October 2010.http://www.satnt.ac.zaam2014ay201
Calciumphosphate scaffolds for bone tissue repair and applications – in vitro characterisation
The purpose of this study was to generate electrospun biphasic nanobioceramic scaffolds for
in vitro testing, ultimately contributing to bone tissue engineering.This abstract was initially
presented at the annual
Biological Sciences
Symposium, presented
under the protection of the
Suid-Afrikaanse Akademie
vir Wetenskap en Kuns. The
symposium was held at the
University of Johannesburg
on 01 October 2011.http://www.satnt.ac.z
In vitro osteoclast-like and osteoblast cells' response to electrospun calcium phosphate biphasic candidate scaffolds for bone tissue engineering
Successful long term bone replacement and repair remain a challenge today. Nanotechnology
has makes it possible to alter materials’ characteristics and therefore possibly improve on the
material itself. In this study, biphasic (hydroxyapatite/β-tricalcium phosphate (HA/β-TCP))
nanobioceramic scaffolds were prepared by the electrospinning technique in order to mimic
the extracellular matrix (ECM). Scaffolds were characterised by scanning electron
microscopy (SEM) and Attentuated Total Reflectance Fourier Transform Infrared (ATRFTIR).
Osteoblasts as well as monocytes that were differentiated into osteoclast-like cells,
were cultured separately on the biphasic bioceramic scaffolds for up to 6 days and the
proliferation, adhesion and cellular response were determined using lactate dehydrogenase
(LDH) cytotoxicity assay, nucleus and cytoskeleton dynamics, analysis of the cell cycle
progression, measurement of the mitochondrial membrane potential and the detection of
phosphatidylserine expression. SEM analysis of the biphasic bioceramic scaffolds revealed
nano fibers spun in a mesh-like scaffold. Results indicate that the biphasic bioceramic
electrospun scaffolds are biocompatible and have no significant negative effects on either
osteoblasts or osteoclast-like cells in vitro.Council for Scientific and Industrial Research (CSIR), Pretoria, South Africa.http://www.springer.com/materials/journal/10853hb201
Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes
Thesis (MSc)--Stellenbosch University, 2005.ENGLISH ABSTRACT: This study describes:
I. The cloning of the CVP 19 gene and construction of the intracellular expression
vector pPIC3.5K-CYP19.
II. The transformation of the yeast, Pichia pastoris with the constructed vector.
III. The expression ofP450arom in Pichia pastoris.
IV. The determination of enzyme activity and isolation of the protein from the Pichia
pastoris cells.
V. The expression of P450c 17 in Pichia pastoris.
VI. The determination of kinetic constants for the conversion of progesterone to
170H-progesterone and 160H-progesterone by P450c17.AFRIKAANSE OPSOMMING: Hierdie studie beskryf:
I. Die klonering van die CVP 19 geen en die konstruksie van die intrasellulêre
uitdrukkingsplasmied, pPIC3.5K-CYPI9.
II. Die transformasie van die gis, Pichia pastoris, met die gekonstrueerde plasmied.
III. Die uitdrukking van aromatase in Pichia pastoris.
IV. Die bepaling van ensiemaktiwiteit en die isolering van die proteïen vanuit Pichia
pastoris.
V. Die uitdrukking van P450c17 in Pichia pastoris.
VI. Die bepaling van kinetiese konstantes vir die omsetting van progesteroon na
170H-progesteroon en 160H-progesteroon deur P450c17
Calciumphosphate scaffolds for bone tissue repair and applications – <i>in vitro<i/> characterisation
<div style="font-size: 12px; font-family: serif; left: 227.349px; top: 259.024px; transform: scale(1.12257, 1); transform-origin: 0% 0% 0px;" dir="ltr">Konvensioneel was sterk, bio-inerte material nog altyd die fokus vir beenvervanging en</div><div style="font-size: 12px; font-family: serif; left: 227.349px; top: 275.157px; transform: scale(1.07552, 1); transform-origin: 0% 0% 0px;" dir="ltr">beenherstel. Bioaktiwiteit van huidige vervangingsmateriale benodig verbetering om vinniger herstel en beter been herkonstruksie in die pasiënt te verseker.</div