Regulatory Mechanism and Application of lncRNAs in Poultry

Long noncoding RNA (lncRNAs) are transcripts greater than 200 nt in length with decreased coding potential and are widespread in all types of biological organisms. lncRNAs can interact with protein, DNA and RNA, respectively, which may participate in the multilevel regulation of transcriptional, post-transcriptional and epigenetic modifications. It is well known that lncRNA, which length is single-stranded non-coding RNA molecule, plays crucial roles in animal growth, development, cell proliferation and differentiation, and other life activities. In this research, we review the regulation mechanism and current research status of lncRNAs in chicken economic traits and disease, which would contribute to further understanding the regulatory mechanisms and application of lncRNAs in poultry

Transcriptional factor regulation network and competitive endogenous RNA (ceRNA) network determining response of esophageal squamous cell carcinomas to neoadjuvant chemoradiotherapy

Background Neoadjuvant chemoradiotherapy (nCRT) followed by surgery benefits survival for patients with esophageal squamous cell carcinomas (ESCC) compared with surgery alone, but the clinical outcomes of nCRT are heterogeneous. This study aimed to elucidate transcriptional factor (TF) regulation network and competitive endogenous RNA (ceRNA) network determining response of ESCC to nCRT. Materials and Methods RNA microarray data of GSE59974 and GSE45670 were analyzed to investigate the significant changes of lincRNAs, miRNAs, mRNAs in responders and non-responders of nCRT in ESCC. Functional and enrichment analyses were conducted by clusterProfiler. The target lincRNAs and mRNAs of miRNAs were predicted by miRWalk. The ceRNA and TF regulatory networks were constructed using Cytoscape. Results Differentially expressed genes between responders and non-responders mainly enriched in biological process including Wnt signaling pathway and regulation of cell development and morphogenesis involved in differentiation. Besides, these genes showed enrichment in molecular function of glycosaminoglycan binding, metalloendopeptidase inhibitor and growth factor activity. KEGG analysis enriched these genes in pathways of neurotrophin signaling pathway, cell adhesion molecules and Wnt signaling pathway. We also constructed ceRNA network and TF network regulating response of ESCC to nCRT. Core regulatory miRNAs were miR-520a, miR-548am, miR-3184, miR-548d, miR-4725, miR-148a, miR-4659a and key regulatory TFs included MBNL1, SLC26A3, BMP4, ZIC1 and ANKRD7. Conclusion We identified significantly altered lincRNAs, miRNAs and mRNAs involved in the nCRT response of ESCC. In addition, the ceRNA regulatory network of lincRNA-miRNA-mRNA and TF regulatory network were constructed, which would elucidate novel molecular mechanisms determining nCRT response of ESCC, thus providing promising clues for clinical therapy

Pores Structure Change Induced by Heat Treatment in Cold-Sprayed Ti6Al4V Coating

In this study, the evolution of pores structure in cold-sprayed Ti6Al4V coating (TC4) was analyzed before and after 600-1100 °C heat treatment. It was found that almost no change happened to pores under the heat treatment temperature below 600 °C. When the heat treatment temperature was increased to 700 °C, the coating recrystallized, and pores turned to spheroid and healed because of the “bridging” effect. Some of the pores coarsened after 800 °C and 900 °C heat treatment. This kind of phenomenon grew severer when the heat treatment temperature increased to 1000 °C and 1100 °C. On the whole, with the increment of temperature, for the coating prepared at relatively low temperature, apparent porosity measured by image analysis method tended to go down first and then up, but it decreased all the time for the coating prepared at relatively high temperature. The reason for this phenomenon was contributed to the bonding state of particles in the coating. Only when there were fewer weakly bonded interfaces, the detachment between the particle interfaces which may be caused by release of residual stress did not occur, and there was no pores expansion and internal connectivity, so the porosity continuously decreased

Evaluation the activity of alveolar echinococcosis: A comparison between 18F-FDG PET and spectral CT

AbstractPurposeTo assess the iodine concentration of hepatic alveolar echinococcosis (HAE) using spectral computed tomography (CT) with comparison of [18F] fluorodeoxyglucose positron-emission tomography (18F-FDG PET), and to estimate the value of spectral CT for evaluation of HAE activity.Materials and methods18 patients with histologically confirmed or clinically proved HAE underwent spectral CT and 18F-FDG PET examinations. After three-phase scanning, the quantitative iodine-based material decomposition images and optimal monochromatic image of spectral CT were reconstructed and iodine concentration (IC) was measured in different organizational structures.Results18F-FDG PET identified increased metabolic activity in the corresponding lesions in 13 patients (13/18, 72.2%). The iodine concentration in marginal zone of lesion were significantly higher than in solid component of lesion and normal liver parenchyma during PVP and VP. The iodine value of edge tissue of the lesion and normal liver and iodine value of normal liver tissues showed statistically significant difference (P < 0.001). There was correlation between IC and SUVmax in marginal zone of HAE lesion, it was highest during PVP (r = 0.873, p < 0.001). There was low correlation between CT values and SUVmax.ConclusionThere was good correlation between spectral CT and 18F-FDG PET. Spectral CT could be recommended as a more practical tool in the clinical routine

Preparation and characterization of magnesium coating deposited by cold spraying

Magnesium (Mg) and its alloys have a great potential as structural materials due to their beneficial combination of high strength to weight ratio, high thermal conductivity and good machinability. However, few literatures about Mg coatings fabricated by cold spraying can be found. In this study, Mg coatings were fabricated by cold spraying, and the microstructure, phase structure, oxygen content and microhardness of the coating prepared under different main gas temperatures were investigated. The critical velocity of the particle was evaluated through numerical simulations. The particle deformation behavior and bonding mechanism were discussed. The result of the oxygen content test shows that the oxygen contents of the coatings did not increase comparing with that of the feedstock powder. The simulation results show that the critical velocity of Mg particles was in a range of 653 m/s to 677 m/s. The observation of the coating fracture morphology shows that the formation of the coating was due to the intensive plastic deformation and mechanical interlocking. The microhardness of the coating increased with the increase of the main gas temperature from 350oC to 450oC due to the decrease of the coating porosity.Comment: 20 pages, 9 figur

Efficient Energy Conversion through Vortex Arrays in the Turbulent Magnetosheath

Turbulence is often enhanced when transmitted through a collisionless plasma shock. We investigate how the enhanced turbulent energy in the Earth's magnetosheath effectively dissipates via vortex arrays. This research topic is of great importance as it relates to particle energization at astrophysical shocks across the universe. Wave modes and intermittent coherent structures are the key candidate mechanisms for energy conversion in turbulent plasmas. Here, by comparing in-situ measurements in the Earth's magnetosheath with a theoretical model, we find the existence of vortex arrays at the transition between the downstream regions of the Earth's bow shock. Vortex arrays consist of quasi-orthogonal kinetic waves and exhibit both high volumetric filling factors and strong local energy conversion, thereby showing a greater dissipative energization than traditional waves and coherent structures. Therefore, we propose that vortex arrays are a promising mechanism for efficient energy conversion in the sheath regions downstream of astrophysical shocks

Cytotoxic secondary metabolites isolated from Penicillium sp. YT2019-3321, an endophytic fungus derived from Lonicera Japonica

IntroductionEndophytic fungi associated with medicinal plants have proven to possess a high potential to produce structurally diverse metabolites, some of which are valuable for medicinal applications. In this study, Penicillium sp. YT2019-3321, an endophytic fungus derived from traditional Chinese medicine Lonicera japonica, was chemically studied.MethodsThe chemical structures of the isolated compounds were established by a correlative interpretation of HRESIMS and NMR spectroscopic data. The optical resolution of (±)-1 by chiral HPLC yielded individual enantiomers (+)-1 and (–)-1, and their stereochemistry were solved by X-ray diffraction crystallography, respectively.Results and discussionEight structurally diversified secondary metabolites, including two previously unreported polyketides, named (±)-chrysoalide B (1) and penicidone E (2), were isolated and identified from Penicillium sp. YT2019-3321. Compound 2 possessed the γ-pyridone nucleus, which is rarely found in natural products. Cytotoxic assay revealed that the new compound 2 demonstrated a dose-dependent cytotoxicity against the human pancreatic tumor cells PATU8988T with the IC50 value of 11.4 μM. Further studies indicated that 2 significantly induced apoptosis of PATU8988T cell lines, characterized by the morphologies abnormity, the reduction of cell number, the upregulation of proportion of apoptotic cells, and the ratio of Bcl-2 to Bax. Our study demonstrates that fungal secondary metabolites may have important significance in the discovery of drug leads