Low <i>Mfn2</i> expression is associated with cell apoptosis in mice's ovarian tissues of POF.

<p>A: Apoptosis was determined by TUNEL in the ovarian tissues (light staining in the cytotrophoblasts of the control group; deeper staining in the cisplatin group); <b>B</b>: The most representative images of western blot for Bcl-2 and Bax; <b>C</b>: Comparison of the Apoptosis Index in the ovarian cells; <b>D</b>: Comparison between Bcl-2 and Bax protein levels; <b>E</b>: Comparison of Mfn2, Bcl-2, and Bax protein levels. *<i>P</i><0.05 vs control group.</p

Morphologic change in ovarian tissues and hormone levels of the two groups.

<p><b>A</b>: Morphology of ovarian tissues was determined by use of HE staining in both the control group and the cisplatin group (blue nuclei, red cytoplasm); <b>B</b>: Comparison of the ovarian weights; <b>C</b>: The comparison of hormone levels (FSH, Estradiol). *<i>P</i><0.05 vs control group.</p

Mfn2 distribution and Mfn2 levels in ovarian tissues of the two groups.

<p><b>A</b>: Mfn2 distribution was exhibited by immunohistochemistry and exclusively located in the cell's cytoplasm; <b>B</b>: The most representative image of western blotting; <b>C</b>: The relative amounts of Mfn2 protein levels in the control and cisplatin group; <b>D</b>: The comparison of Mfn2 mRNA levels in the ovarian tissue. *<i>P</i><0.05 vs control group.</p

Mitochondrial dysfunction is correlated with reduced Mfn2 levels in ovarian tissue from the two groups.

<p><b>A</b>: Ovarian tissue ultramicrostructure as detected by TEM (Aa: the most representative image of mitochondrial morphology and cristae in the control group; Ab: the most representative image of mitochondrial membrane and cristae in the cisplatin group; Ac: the fibrosis of ovarian tissues in the cisplatin group; Ad: the fat accumulation in ovarian tissues of the cisplatin group); <b>B</b>: The mitochondrial membrane potential (ΔΨm) as detected by using JC-1; <b>C</b>: Statistical analyses of the red and green fluorescence intensity ratios; <b>D</b>: ATP levels as determined by phosphomolybdic acid colorimetric method in the two groups. *<i>P</i><0.05 vs control group.</p

Chemoenzymatic Synthesis of Cholesterol‑<i>g</i>‑Poly(amine-<i>co</i>-ester) Amphiphilic Copolymer as a Carrier for miR-23b Delivery

The lipase-catalyzed polymerization of <i>N</i>-methyldiethanolamine, diethyl sebacate and ω-pentadecanolide was performed to construct a cationic poly­(amine-<i>co</i>-ester), and a hydrophobic <i>N</i>-(2-bromoethyl)­carbamoyl cholesterol was then grafted onto its main chain through a quaternization reaction to prepare the amphiphilic copolymer Chol-<i>g</i>-PMSC-PPDL. The copolymer efficiently bound and condensed miR-23b to form stable nanocomplexes, which showed favorable cellular uptake and miR-23b transfection efficacy due to the introduction of the hydrophobic segment. After miR-23b delivery, an obvious inhibition of cell proliferation could be induced, which was attributed to the induction of cell apoptosis and cell cycle arrest. Moreover, the carrier-mediated miR-23b delivery could inhibit the migration and invasion of tumor cells. Overall, the work provides a novel chemoenzymatic strategy for constructing biodegradable and biocompatible poly­(amine-<i>co</i>-ester) derivatives, which are promising carriers for oligonucleotide delivery to achieve tumor gene therapy