p450scc protein expression in normal pregnancy and preeclampsia group relative optical density.

<p>t = 3.528; 95% CI (0.9424,3.3623).</p

Linear multiple regression result of P450scc protein expression and clinical parameters.

<p>(A) CYP11A expression negatively correlated to INR (P = 0.095). (B) CYP11A expression positively correlated to Plt (P = 0.018). (C) CYP11A expression positively correlated to ALT (P = 0.02).</p

P450scc protein expression in preeclampsia group was significantly higher than normal control.

<p>Left panel: representative western blot result of the placenta samples, Right panel: statistic of western blot result from 53 normal samples and 59 preeclampsia samples (p<0.05).</p

Enhanced apoptosis in CYP11A overexpressed HTR-8/SVneo cells.

<p>(A) TUNEL staining shows increased positive signals in CYP11A overexpressed HTR-8/SVneo cells compared to vector control when cultured in hypoxia condition. (B) Western blot result showed increased cleaved caspase-3 expression in CYP11A overexpressed HTR-8/SVneo compared to vector control cells. The image shown is representative result of two independant experiments.</p

of CYP 11A mRNA in normal pregnancy group, severe pre-eclampsia group compared with the relative optical density of expression in mild preeclampsia.

<p>Normal pregnancy group with severe preeclampsia group: p = 0.015,95% CI (−0.5388, −0.0595).</p><p>Normal pregnancy group compared with mild preeclampsia: p = 0.241,95% CI (−0.6510, 0.1565).</p><p>Mild preeclampsia and severe preeclampsia group: p = 0.727,95% CI (− 0.4656, 0.3259).</p

p450scc protein expression multiple regression analysis result.

a<p>Dependent Variable: lgp450db.</p

Immunohistochemistry staining of P450scc in placenta tissue.

<p>(A) Upper panel: Visible P450scc positive expression located in the cytoplasm of syncytiotrophoblast of placenta (arrow). Left: negative control without primary antibody. Right: P450scc (CYP11A) staining; Manification 600x; scale bar = 40 uM. Down panel: the higher manification pictures of the insert part of the above figures (B) Western blot shows that there is no significant different of CYP11A expression between first and third trimester placentas.</p

Expression of CFTR in rat prostate epithelial cells.

<p>(A) Immunohistochemical staining of CFTR in SD rat prostate with negative control in the absence of primary antibody. CFTR was expressed at the apical surface of rat ventral prostate epithelium. Scale bar: 100 µm. (B) CFTR transcript was detected by RT-PCR in cultured rat prostate epithelial cells with predicted amplification products at 481 bp. (C) CFTR protein was detected in rat prostate epithelial cells by Western blotting which recognizes a band at MW 160 kDa. (D) Phase contrast image (left) and immunofluorescence staining of cytokeratin 5&8 (middle, green) or CFTR (right, green) in rat prostate epithelial cells. Cell nuclei were counterstained with DAPI (blue). Scale bar: 100 µm.</p

Expression of CFTR and CAII in human hyperplasia prostate with inflammation.

<p>CFTR (A, B) and CAII (C, D) were detected in human hyperplasia tissues. There was lymphocytes infiltration (Yellow arrow, B, D) in the inflamed area of the clinical prostate hyperplasia samples. Note that the expression of CFTR and CAII was stronger in the area with lymphocytes infiltration (Red arrow, B, D) than those without infiltration (Red arrow, A, C). (E) Negative control. Scale bar: 50 µm.</p

Solutions for different concentration of HCO₃⁻.

<p>Solutions for different concentration of HCO₃⁻.</p