Additional file 1: of Quality assurance target for community-based breast cancer screening in China: a model simulation

Figure S1. Shows the age-specific incidence rate of breast cancer in USA, rural and urban area of China, while Table S1. includes the information of cost-utility analysis of different screening strategy among 40–69 years old women. (DOCX 28 kb

<i>Trps1</i> Differentially Modulates the Bone Mineral Density between Male and Female Mice and Its Polymorphism Associates with BMD Differently between Women and Men

<div><p>The objective of our study was to identify genetic factors that regulate bone mineral density (BMD) in mice using well defined recombinant inbred strains. For this purpose we chose the BXD recombinant inbred (RI) strains derived from progeny of the C57BL/6J (B6) and DBA/2J (D2) progenitor strains. We sampled both male and female mice (∼4 each) of 46 strains at 3 months-of-age, measured their BMD, and conducted QTL mapping. The data were analyzed to identify candidates genes contained within the most significant quantitative trait locus (QTL). Evaluation of candidate genes included functional assessment, single nucleotide polymorphism (SNP) genotyping and direct sequencing. We established that there was a QTL for BMD in males on chromosome 15 that has the impact larger than QTLs on all other chromosomes. The QTL on chromosome 15 was narrowed to a genomic region between 38 Mbp and 52 Mbp. By examining transcripts within this region, we found an important candidate gene: trichorhinophalangeal syndrome, type I (<i>Trps1</i>). SNP analysis identified a nonsynonymous SNP (<i>rs32398060</i>) in <i>Trps1</i> that co-segregated with bone mineral density. Analysis of association between this SNP within TRPS1 and BMD in a human population confirmed its significance.</p></div

TRPS1 alleles and BMD between women and men in human population.

<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084485#pone-0084485-g004" target="_blank">Figure 4A</a>. The linkage disequilibrium (LD) plot of the SNPs within the TRPS1 gene. It was plotted by the software Haploview. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084485#pone-0084485-g004" target="_blank">Figure 4B</a>. P values of 6310 among women and men. The lower the P value is, the stronger association between SNP allele and BMD is. Y-bar indicates the P values of each SNP in women and in men with different colors. X-bar indicates the number of SNP. Different color in many individual bars can be seen because the different P values between women and men in those SNP alleles. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084485#pone-0084485-g004" target="_blank">Figure 4C</a>. Potential detection of QTL using data of BMD of the femur in female mice.</p

<i>Trps1</i> as the candidate gene for BMD in male.

<p>BMD of “G” and “C” genotypes of femur and tibia in male mice.</p