Preservation of (−)-Epigallocatechin-3-gallate Antioxidant Properties Loaded in Heat Treated β-Lactoglobulin Nanoparticles

(−)-Epigallocatechin-3-gallate (EGCG) was loaded in heat treated β-lactoglobulin (β-Lg) for the preservation of antioxidant activity. The effects of pH (2.5–7.0), the heating temperature of β-Lg (30–85 °C), the molar ratio of β-Lg to EGCG (1:2–1:32), and the β-Lg concentration (1–10 mg/mL) on the properties of β-Lg–EGCG complexes were studied. All four factors significantly influenced the particle size, the ζ-potential, and the entrapment efficiency of EGCG and EGCG loading in β-Lg particles. A stable and clear solution system could be obtained at pH 6.4–7.0. The highest protection of EGCG antioxidant activity was obtained with β-Lg heated at 85 °C and the molar ratio of 1:2 (β-Lg: EGCG). β-Lg–EGCG complexes were found to have the same secondary structure as native β-Lg

Additional file 4: Figure S2. of A genome-wide expression profile analysis reveals active genes and pathways coping with phosphate starvation in soybean

Identification of differently up-regulated genes in roots between different soybean accessions. (DOC 26 kb

Additional file 1: Figure S1. of A genome-wide expression profile analysis reveals active genes and pathways coping with phosphate starvation in soybean

Roots morphological of soybean accession CD and YH under low-P condition (0.005 mmol/L P) (A) Root morphological before low-P treatments (B) Root morphological after 10 days of low-P treatments. (DOC 190 kb

Additional file 2: Table S1. of A genome-wide expression profile analysis reveals active genes and pathways coping with phosphate starvation in soybean

KEGG and Go enrichment analysis of differently expressed genes. (a) 317 DEGs between different P treatments (b) 195 DEGs between different materials (c) 253 DEGs between different tissues (d) 85 overlapping genes between the 195 marked DEGs between CD and YH and 317 DEGs in roots and leaves (e) 68 overlapping genes between the 253 marked DEGs between roots and leaves and 317 DEGs in roots and leaves (f) 42 common DEGs. (XLS 86 kb

Additional file 5: Table S3. of A genome-wide expression profile analysis reveals active genes and pathways coping with phosphate starvation in soybean

10 genes showing opposite expression patterns in roots between different soybean accessions. (DOC 42 kb

Additional file 3: Table S2. of A genome-wide expression profile analysis reveals active genes and pathways coping with phosphate starvation in soybean

The list of DEGs between different P treatments in the roots and leaves of CD and YH. (XLS 55 kb