Forest plot of HDL-c levels among different diets.

<p>The squares and horizontal lines correspond to the study-specific SMD and 95% CI. The area of the squares reflects the study-specific weight (inverse of the variance). The diamond represents the pooled SMD and 95% CI. % weighted for random-effects.</p

Characteristics of literatures included in the meta-analysis and observational studies evaluating the effects of diets in HDL (mmol/l)¹.

1<p>Data were expressed as Mean ± SD; To convert from mg/dl to mmol/L, multiply by 0.02586;</p>2<p>Euro-Amer (Europe and North America): UK, USA, Slovak, Australia, Germany, and Greece; Asia-Latin (Asia and Latin America): Brazil and China.</p>3<p>Cross: cross-sectional studies; Cohort: cohort studies.</p

Results of search for eligible studies.

<p>Results of search for eligible studies.</p

Verification of PSMB1 expression in PA under prolonged hypoxia.

<p><b>A:</b> Zoomed-in view of protein spot 28 in the 2DE gel in Fig. 1. <b>B:</b> Relative protein expression of PSMB1 in the 2DE gel. The normoxia group was considered as 100% and the relative fold-change in protein level is shown by spot density. <b>C:</b> Relative mRNA levels for PSMB1 from the PA of rats exposed to 10% O₂ for 21 days compared with normoxia control. Bar values are mean ± SEM (n = 5 in each group). *<i>P</i><0.05 versus respective normoxia control. <b>D:</b> Protein levels of PSMB1 measured by Western blot in PA isolated from rats exposed to hypoxia (10% O₂, 21 days) or normoxia. <b>E:</b> Band intensity of PSMB1 normalized to α-actin. Mean values ± SE were calculated from three independent samples. *<i>P</i><0.01 versus normoxia.</p

Verification of PSMB6 expression in the PA under prolonged hypoxia.

<p><b>A:</b> Zoomed-in view of protein spot 14 in the 2DE gel in Fig. 1. <b>B:</b> Relative protein expression of PSMB6 in the 2DE gel. The normoxia group was considered as 100% and the relative fold-change in protein level is shown by spot density. <b>C:</b> Relative mRNA levels for PSMB6 from the PA of rats exposed to 10% O₂ for 21 days compared with the normoxia control. Bar values are mean ± SEM (n = 5 in each group). *<i>P</i><0.05 versus respective normoxia control. <b>D:</b> Protein levels of PSMB6 measured by Western blot in the PA isolated from rats exposed to hypoxia (10% O₂, 21 days) or normoxia. <b>E:</b> Band intensity of PSMB6 normalized to α-actin. Mean values ± SEM were calculated from three independent samples. *<i>P</i><0.01 versus normoxia.</p

Effects of PSMB6 siRNA on the proliferation of PASMCs induced by prolonged hypoxia.

<p><b>A</b> and <b>B</b>: The effects of PSMB6 siRNA (siPSMB6) on PSMB6 protein expression in PASMCs. PASMCs were transfected with 25 nM of non-targeting control siRNA (siNT) or siPSMB6 for 6 h in serum-free SMBM. PASMCs were then exposed to siRNA for 72 h before subsequent analysis with Western blotting. <b>C</b>: Effects of prolonged hypoxia and siPSMB6 on the proliferation of PASMCs by BrdU assay. PASMCs were treated with normoxia or prolonged hypoxia (4% O₂, 60 h) with or without siPSMB6 treatment at dosages of 25 nM. Bar values are means ± SEM. *<i>P</i><0.05 compared with normoxia nontargeting siRNA. §<i>P</i><0.05 compared with hypoxic nontargeting siRNA (<i>n</i> = 3 in each group).</p

Effect of MG132 on proliferation of PASMCs induced by prolonged hypoxia.

<p>PASMCs were treated with normoxia or prolonged hypoxia (4% O₂, 60 h) with or without MG132 at various dosages (0.01 to 0.2 µM). The bar graph represents the effects of prolonged hypoxia and MG132 on the proliferation of PASMCs by MTT assay. Bar values are means ± SEM. *<i>P</i><0.05 compared with normoxia control. §<i>P</i><0.05 compared with hypoxic control (n = 3 in each group).</p

Up-regulated proteins identified by MS or MS/MS and database searches in the hypoxia group.

a<p>Protein spot numbers on 2DE gel.</p>b<p>Accession numbers from NCBI database (accessible at <a href="http://www.ncbi.nlm.nih.gov/" target="_blank">http://www.ncbi.nlm.nih.gov/</a>) or the Swiss-Prot Protein Database (<a href="http://web.expasy.org/docs/swiss-prot_guideline.html" target="_blank">http://web.expasy.org/docs/swiss-prot_guideline.html</a>).</p>c<p>Theoretical molecular mass.</p>d<p>Theoretical PI.</p>e<p>MASCOT protein score (based on combined MS and MS/MS spectra) of greater than 64 (<i>P</i><0.05) or the total ion score (based on MS/MS spectra) of greater than 30 (<i>P</i><0.05).</p

Up-regulated proteins identified by MS or MS/MS and database searches in the normoxia group.

a<p>Protein spot numbers on 2DE gel.</p>b<p>Accession numbers from NCBI database (accessible at <a href="http://www.ncbi.nlm.nih.gov/" target="_blank">http://www.ncbi.nlm.nih.gov/</a>) or the Swiss-Prot Protein Database (<a href="http://web.expasy.org/docs/swiss-prot_guideline.html" target="_blank">http://web.expasy.org/docs/swiss-prot_guideline.html</a>).</p>c<p>Theoretical molecular mass.</p>d<p>Theoretical PI.</p>e<p>MASCOT protein score (based on combined MS and MS/MS spectra) of greater than 64 (<i>P</i><0.05) or the total ion score (based on MS/MS spectra) of greater than 30 (<i>P</i><0.05).</p

Two-dimensional electrophoresis maps.

<p>The two-dimensional electrophoresis maps represent the separation view of the extracted proteins in the distal PA isolated from normoxic and CHPH rats (10% O₂, 21 days). PA whole protein (190 µg) was separated on y1y∼10 IPG strip in the first dimension and 12% SDS-PAGE in the second dimension. The up-regulated protein spots are marked in the gels. There are 5 spots in the normoxia group and 24 spots in the hypoxia group. The molecular markers are shown on the right of the gel. The PI information is shown on the bottom of both gels.</p