Impact of cytotoxic T lymphocytes immunotherapy on prognosis of colorectal cancer patients

BackgroundExpansion and activation of cytotoxic T lymphocytes (CTLs) in vitro represents a promising immunotherapeutic strategy, and CTLs can be primed by dendritic cells (DCs) loaded with tumor-associated antigens (TAAs) transformed by recombinant adeno-associated virus (rAAV). This study aimed to explore the impact of rAAV-DC-induced CTLs on prognosis of CRC and to explore factors associated with prognosis.MethodsThis prospective observational study included patients operated for CRC at Yan’an Hospital Affiliated to Kunming Medical University between 2016 and 2019. The primary outcome was progression-free survival (PFS), secondary outcomes were overall survival (OS) and adverse events. Totally 49 cases were included, with 29 and 20 administered rAAV-DC-induced CTL and chemotherapy, respectively.ResultsAfter 37-69 months of follow-up (median, 54 months), OS (P=0.0596) and PFS (P=0.0788) were comparable between two groups. Mild fever occurred in 2 (6.9%) patients administered CTL infusion. All the chemotherapy group experienced mild-to-moderate adverse effects, including vasculitis (n=20, 100%), vomiting (n=5, 25%), nausea (n=17, 85%) and fatigue (n=17, 85%).ConclusionsLymphatic metastasis (hazard ratio [HR]=4.498, 95% confidence interval [CI]: 1.290-15.676; P=0.018) and lower HLA-I expression (HR=0.294, 95%CI: 0.089-0.965; P=0.044) were associated with poor OS in the CTL group. CTLs induced by rAAV-DCs might achieve comparable effectiveness in CRC patients compare to chemotherapy, cases with high tumor-associated HLA-I expression and no lymphatic metastasis were more likely to benefit from CTLs

A High Resolution Genetic Map Anchoring Scaffolds of the Sequenced Watermelon Genome

As part of our ongoing efforts to sequence and map the watermelon (Citrullus spp.) genome, we have constructed a high density genetic linkage map. The map positioned 234 watermelon genome sequence scaffolds (an average size of 1.41 Mb) that cover about 330 Mb and account for 93.5% of the 353 Mb of the assembled genomic sequences of the elite Chinese watermelon line 97103 (Citrullus lanatus var. lanatus). The genetic map was constructed using an F8 population of 103 recombinant inbred lines (RILs). The RILs are derived from a cross between the line 97103 and the United States Plant Introduction (PI) 296341-FR (C. lanatus var. citroides) that contains resistance to fusarium wilt (races 0, 1, and 2). The genetic map consists of eleven linkage groups that include 698 simple sequence repeat (SSR), 219 insertion-deletion (InDel) and 36 structure variation (SV) markers and spans ∼800 cM with a mean marker interval of 0.8 cM. Using fluorescent in situ hybridization (FISH) with 11 BACs that produced chromosome-specifc signals, we have depicted watermelon chromosomes that correspond to the eleven linkage groups constructed in this study. The high resolution genetic map developed here should be a useful platform for the assembly of the watermelon genome, for the development of sequence-based markers used in breeding programs, and for the identification of genes associated with important agricultural traits

Potential of Core-Collapse Supernova Neutrino Detection at JUNO

JUNO is an underground neutrino observatory under construction in Jiangmen, China. It uses 20kton liquid scintillator as target, which enables it to detect supernova burst neutrinos of a large statistics for the next galactic core-collapse supernova (CCSN) and also pre-supernova neutrinos from the nearby CCSN progenitors. All flavors of supernova burst neutrinos can be detected by JUNO via several interaction channels, including inverse beta decay, elastic scattering on electron and proton, interactions on C12 nuclei, etc. This retains the possibility for JUNO to reconstruct the energy spectra of supernova burst neutrinos of all flavors. The real time monitoring systems based on FPGA and DAQ are under development in JUNO, which allow prompt alert and trigger-less data acquisition of CCSN events. The alert performances of both monitoring systems have been thoroughly studied using simulations. Moreover, once a CCSN is tagged, the system can give fast characterizations, such as directionality and light curve

Detection of the Diffuse Supernova Neutrino Background with JUNO

As an underground multi-purpose neutrino detector with 20 kton liquid scintillator, Jiangmen Underground Neutrino Observatory (JUNO) is competitive with and complementary to the water-Cherenkov detectors on the search for the diffuse supernova neutrino background (DSNB). Typical supernova models predict 2-4 events per year within the optimal observation window in the JUNO detector. The dominant background is from the neutral-current (NC) interaction of atmospheric neutrinos with 12C nuclei, which surpasses the DSNB by more than one order of magnitude. We evaluated the systematic uncertainty of NC background from the spread of a variety of data-driven models and further developed a method to determine NC background within 15\% with {\it{in}} {\it{situ}} measurements after ten years of running. Besides, the NC-like backgrounds can be effectively suppressed by the intrinsic pulse-shape discrimination (PSD) capabilities of liquid scintillators. In this talk, I will present in detail the improvements on NC background uncertainty evaluation, PSD discriminator development, and finally, the potential of DSNB sensitivity in JUNO

Real-time Monitoring for the Next Core-Collapse Supernova in JUNO

Core-collapse supernova (CCSN) is one of the most energetic astrophysical events in the Universe. The early and prompt detection of neutrinos before (pre-SN) and during the SN burst is a unique opportunity to realize the multi-messenger observation of the CCSN events. In this work, we describe the monitoring concept and present the sensitivity of the system to the pre-SN and SN neutrinos at the Jiangmen Underground Neutrino Observatory (JUNO), which is a 20 kton liquid scintillator detector under construction in South China. The real-time monitoring system is designed with both the prompt monitors on the electronic board and online monitors at the data acquisition stage, in order to ensure both the alert speed and alert coverage of progenitor stars. By assuming a false alert rate of 1 per year, this monitoring system can be sensitive to the pre-SN neutrinos up to the distance of about 1.6 (0.9) kpc and SN neutrinos up to about 370 (360) kpc for a progenitor mass of 30$M_{\odot}$ for the case of normal (inverted) mass ordering. The pointing ability of the CCSN is evaluated by using the accumulated event anisotropy of the inverse beta decay interactions from pre-SN or SN neutrinos, which, along with the early alert, can play important roles for the followup multi-messenger observations of the next Galactic or nearby extragalactic CCSN.Comment: 24 pages, 9 figure

Feature Correspondences Increase and Hybrid Terms Optimization Warp for Image Stitching

Feature detection and correct matching are the basis of the image stitching process. Whether the matching is correct and the number of matches directly affect the quality of the final stitching results. At present, almost all image stitching methods use SIFT+RANSAC pattern to extract and match feature points. However, it is difficult to obtain sufficient correct matching points in low-textured or repetitively-textured regions, resulting in insufficient matching points in the overlapping region, and this further leads to the warping model being estimated erroneously. In this paper, we propose a novel and flexible approach by increasing feature correspondences and optimizing hybrid terms. It can obtain sufficient correct feature correspondences in the overlapping region with low-textured or repetitively-textured areas to eliminate misalignment. When a weak texture and large parallax coexist in the overlapping region, the alignment and distortion often restrict each other and are difficult to balance. Accurate alignment is often accompanied by projection distortion and perspective distortion. Regarding this, we propose hybrid terms optimization warp, which combines global similarity transformations on the basis of initial global homography and estimates the optimal warping by adjusting various term parameters. By doing this, we can mitigate projection distortion and perspective distortion, while effectively balancing alignment and distortion. The experimental results demonstrate that the proposed method outperforms the state-of-the-art in accurate alignment on images with low-textured areas in the overlapping region, and the stitching results have less perspective and projection distortion

Numerical Simulation on the Transient Temperature Field of Anode Rodding in Aluminium Reduction Cells

The temperature field of the anode rodding process is the root of most of problems related to the anode. A 3D transient thermal finite element model was developed, based on ANSYS in this paper. The temperature field of the rodding process was investigated using this model. The results were presented and analyzed in detail, including the transient temperature fields of different components in the anode, the characteristics of the cast iron solidification, and the cooling rate distribution. The conclusions were as follows. When the stub hole was filled up at 10 s, 30% by volume of cast iron was below the liquidus temperature. At 70 s, the cast iron solidified completely. The cast iron close to the stub solidified prior to that close to the carbon. The solidification mode was intermediate-freezing. The solidifying sequence was vertically from bottom to top, indicating that shrinkage cavities were less likely to occur. The temperature of the stub was higher than the carbon, and portion at high temperature was concentrated around the stub hole. The cooling rate during solidification was 4.3 °C s−1 to 43.7 °C s−1, and the gradient on the radial direction was relatively obvious

A broadscale shift in antarctic temperature trends

During the second half of the twentieth century, the Antarctic Surface Air Temperature (SAT) trends are characterized by fast warming over West Antarctica but mild cooling over East Antarctica. However, after 2000, the warming over several stations in the Antarctic Peninsula slowed down, whereas the South Pole experienced fast warming. These reversed SAT trends show strong regionality and seasonality, together with large uncertainty and disagreement among different observational and reanalysis datasets, which makes it difficult to achieve a comprehensive understanding of the multi-decadal Antarctic SAT trend and its reversal. In this study, we use the Combined Maximum Covariance Analysis (CMCA) method to extract the most coherent modes of the Antarctic SAT trends among six reanalysis datasets and 26 station-based observations. Further analysis shows that the reversals of the SAT trends before and after 2000, especially for austral spring and summer, are mainly attributed to the reversed trends of the leading CMCA modes and their related atmospheric circulation and thermal advection patterns over Antarctica. For austral spring, the reversal of the west-warming-east-cooling pattern over Antarctica is closely related to the changes of thermal advection induced by the anomalous circulation center over the Antarctic Peninsula–Weddell Sea region. For summer, the post-2000 reversal of the Antarctic Peninsula-warming-East Antarctic-cooling is attributed to the stratospheric ozone recovery over the Antarctic, and the associated adjustment of the southern annular mode. The CMCA decomposition better combines the information from different measurements, clarifying the long-term SAT trend and its reversal for different seasons