A platinum shell for ultraslow ligand exchange: unmodified DNA adsorbing more stably on platinum than thiol and dithiol on gold

Due to the ultraslow ligand exchange rate on Pt, non-thiolated DNA is adsorbed on platinum nanoparticles (PtNPs) more stably than thiolated and even dithiolated DNA on AuNPs. Adsorption kinetics, capacity and stability are systematically compared as a function of DNA sequence. The Pt conjugates can tolerate extreme pH, salt, and thiol molecules. Taking advantage of the optical properties of AuNPs and the extreme stability of DNA on PtNPs, DNA-functionalized Au@Pt NPs are prepared using a cost-effective and more stable bioconjugation method. The DNA-directed assembly of non-thiolated DNA conjugates is also demonstrated.University of Waterloo || Natural Sciences and Engineering Research Council || Foundation for Shenghua Scholar of Central South University || National Natural Science Foundation of China || Grant No. 21301195 China Scholarship Council || CSC, Grant No. 20140637011

No Fun no Use? The Impact of Gamification on User’s Continuous Usage Intention toward E-Business App

Gamification as an internal incentive method has been widely adopted in practice. Compared with the traditional external rewards, internal rewards can stimulate the enthusiasm of users. This paper，therefore，explores and constructs a model on how gamification (herein composed of three dimensions: sense of achievement, social influence and sense of ownership) affects user’s continuance intention from the perspective of intrinsic motivation (herein composed of three dimensions: self-presentation, entertainment and self-efficacy). Through the multiple regression analysis of 456 e-shoppers sample data by using the software of SMARTPLS 3.0, we draw the following five conclusions: gamification was positively correlated with self-presentation, entertainment and self-efficacy; sense of achievement, social influence and sense of ownership, significantly positively affected self-presentation, entertainment and self-efficacy; there is no correlation between self-presentation and users\u27 continuance intention; entertainment and self-efficacy are positively correlated with users\u27 continuance intention; and entertainment and self-efficacy play a mediation role between gamification and users\u27 continuance intention

Tandem Phosphorothioate Modifications for DNA Adsorption Strength and Polarity Control on Gold Nanoparticles

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Applied Materials & Interfaces, copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Zhou, W., Wang, F., Ding, J., & Liu, J. (2014). Tandem Phosphorothioate Modifications for DNA Adsorption Strength and Polarity Control on Gold Nanoparticles. ACS Applied Materials & Interfaces, 6(17), 14795–14800. https://doi.org/10.1021/am504791bUnmodified DNA was recently used to functionalize gold nanoparticles via DNA base adsorption. Compared to thiolated DNA, however, the application of unmodified DNA is limited by the lack of sequence generality, adsorption polarity control and poor adsorption stability. We report that these problems can be solved using phosphorothioate (PS) DNA. PS DNA binds to gold mainly via the sulfur atom and is thus less sequence dependent. The adsorption affinity is ranked to be thiol > PS > adenine > thymine. Tandem PS improves adsorption strength, allows tunable DNA density, and the resulting conjugates are functional at a low cost.University of Waterloo || Natural Sciences and Engineering Research Council || Foundation for Shenghua Scholar of Central South University || National Natural Science Foundation of China || Grant No. 2130119

EDIS: Entity-Driven Image Search over Multimodal Web Content

Making image retrieval methods practical for real-world search applications requires significant progress in dataset scales, entity comprehension, and multimodal information fusion. In this work, we introduce \textbf{E}ntity-\textbf{D}riven \textbf{I}mage \textbf{S}earch (EDIS), a challenging dataset for cross-modal image search in the news domain. EDIS consists of 1 million web images from actual search engine results and curated datasets, with each image paired with a textual description. Unlike datasets that assume a small set of single-modality candidates, EDIS reflects real-world web image search scenarios by including a million multimodal image-text pairs as candidates. EDIS encourages the development of retrieval models that simultaneously address cross-modal information fusion and matching. To achieve accurate ranking results, a model must: 1) understand named entities and events from text queries, 2) ground entities onto images or text descriptions, and 3) effectively fuse textual and visual representations. Our experimental results show that EDIS challenges state-of-the-art methods with dense entities and a large-scale candidate set. The ablation study also proves that fusing textual features with visual features is critical in improving retrieval results

Aptamer-based biosensors for biomedical diagnostics

Aptamers are single-stranded nucleic acids that selectively bind to target molecules. Most aptamers are obtained through a combinatorial biology technique called SELEX. Since aptamers can be isolated to bind to almost any molecule of choice, can be readily modified at arbitrary positions and they possess predictable secondary structures, this platform technology shows great promise in biosensor development. Over the past two decades, more than one thousand papers have been published on aptamer-based biosensors. Given this progress, the application of aptamer technology in biomedical diagnosis is still in a quite preliminary stage. Most previous work involves only a few model aptamers to demonstrate the sensing concept with limited biomedical impact. This Critical Review aims to summarize progress that might enable practical applications of aptamers for biological samples. First, general sensing strategies based on the unique properties of aptamers are summarized. Each strategy can be coupled to various signaling methods. Among these, a few detection methods including fluorescence lifetime, flow cytometry, upconverting nanoparticles, nanoflare technology, magnetic resonance imaging, electronic aptamer-based sensors, and lateral flow devices have been discussed in more detail since they are more likely to work in a complex sample matrix. The current limitations of this field include the lack of high quality aptamers for clinically important targets. In addition, the aptamer technology has to be extensively tested in a clinical sample matrix to establish reliability and accuracy. Future directions are also speculated to overcome these challenges.University of Waterloo || Natural Sciences and Engineering Research Council || Foundation for Shenghua Scholar of Central South University |

NF-Y Recruits Both Transcription Activator and Repressor to Modulate Tissue- and Developmental Stage-Specific Expression of Human γ-Globin Gene

The human embryonic, fetal and adult β-like globin genes provide a paradigm for tissue- and developmental stage-specific gene regulation. The fetal γ-globin gene is expressed in fetal erythroid cells but is repressed in adult erythroid cells. The molecular mechanism underlying this transcriptional switch during erythroid development is not completely understood. Here, we used a combination of in vitro and in vivo assays to dissect the molecular assemblies of the active and the repressed proximal γ-globin promoter complexes in K562 human erythroleukemia cell line and primary human fetal and adult erythroid cells. We found that the proximal γ-globin promoter complex is assembled by a developmentally regulated, general transcription activator NF-Y bound strongly at the tandem CCAAT motifs near the TATA box. NF-Y recruits to neighboring DNA motifs the developmentally regulated, erythroid transcription activator GATA-2 and general repressor BCL11A, which in turn recruit erythroid repressor GATA-1 and general repressor COUP-TFII to form respectively the NF-Y/GATA-2 transcription activator hub and the BCL11A/COUP-TFII/GATA-1 transcription repressor hub. Both the activator and the repressor hubs are present in both the active and the repressed γ-globin promoter complexes in fetal and adult erythroid cells. Through changes in their levels and respective interactions with the co-activators and co-repressors during erythroid development, the activator and the repressor hubs modulate erythroid- and developmental stage-specific transcription of γ-globin gene.</p