Additional file 2: Figure S1. of Low expression of galectin-3 is associated with poor survival in node-positive breast cancers and mesenchymal phenotype in breast cancer stem cells

The Cancer Genome Atlas (TCGA) data show the gene expression of Gal3 (LGALS3) in normal (no value), ductal breast carcinoma in situ and invasive ductal breast carcinoma (A) or normal (no value), primary site and metastatic site of human breast cancer samples (B). (C) Western blot analysis of whole cell lysates of GI-101A and its derivatives (GI-LM2, GI-LM2C, GI-LM2G) on estrogen receptor (ER) expression. Figure S2 (A) Immunofluorescence staining of GI-LM2C (upper row) and GI-LM2G spheres (lower row) for Gal3 (red), E-cadherin (CDH1, green), and vimentin (gray). (B) Immunofluorescence staining of the same cell lines for cytokeratin 18 (red) and vimentin (green). Counterstaining with DAPI (blue) was used to visualize cell nuclei. Figure S3 (A) Flow cytometric analysis shows that Gal3-positive populations (in red) of the same cell line consistently contain a lower BCSC pool than Gal3-negative populations (in green). (B) Correlation of Gal3 with CD24 and EpCAM expression is listed in a table. Figure S4 (A) Brightfield pictures of spheres in low magnification. Figure is related with Fig. 3a. (B) Sphere-formation assay and its quantification of GI-101A, GI-101A after knockout of Gal3 (GI-101A-G) as well as derivatives GI-LM2C and GI-LM2G. (C) Western blot of whole cell lysates of GI-LM2C and GI-LM2G for Wnt targets Axin2 and Tcf4. Loading control β-actin was used. The same membrane is used in Fig. S1C. (PPTX 2636 kb

Additional file 4: Figure S2. of HDAC6 activity is a non-oncogene addiction hub for inflammatory breast cancers

Inhibition of HDAC6 activity by small molecules in vitro and in vivo. The western blots show the accumulation of Ac-Îą-tubulin when SUM149 cells were treated with Ricolinostat and Tubastatin-A in vitro (a) and in vivo (b). (EPS 783 kb

Additional file 6: Figure S4. of HDAC6 activity is a non-oncogene addiction hub for inflammatory breast cancers

Response to paclitaxel treatment in breast cancer cell line models. The bars indicates the normalized survival after different breast cancer cell lines (inflammatory breast cancer (IBC) and non-IBC) were treated for two doubling times with 10 uM of paclitaxel. Expression change of HDAC6 regulon network over time upon Ricolinostat treatment. (EPS 713 kb

Additional file 2: Figure S1. of HDAC6 activity is a non-oncogene addiction hub for inflammatory breast cancers

Quality control studies of the shRNA screens. a Representative image showing the Pearson and Spearman correlation among the triplicates for T = 10 in the SUM149 cell line. b GO-term and KEGG-pathway analyses using genes commonly depleted in several cell lines (p <0.05 in >=3 cell lines, 2,555 genes) show enrichment of genes related to essential functions. c Essential genes depleted in our shRNA screen cell lines overlapped significantly with compiled screens across 72 cell lines and subtypes of cancer (Fisher’s exact test). (EPS 3172 kb