Additional file 1: of Collection of non-meconium stool on fecal occult blood cards is an effective method for fecal microbiota studies in infants

Table S1: Software and R libraries used in the analysis. Table S2: P values from Adonis tests for different contrasts with different rarefaction cutoffs and different distance measures. Figure S1A: Rarefaction plot of number of assigned OTUs versus number of sequences from different storage conditions in meconium samples. The vertical line indicates the number of sequences used for the single rarefaction in analyses. Figure S1B: Rarefaction plot of number of assigned OTUs versus number of sequences from different storage conditions in stool samples. The vertical line indicates the number of sequences used for the single rarefaction in analyses. Figure S2: PCoA plots for all samples. Figure S3A. PCoA plots with Bray–Curtis, Jensen–Shannon divergence, unweighted UniFrac, and weighted UniFrac distance measures for meconium samples. Figure S3B. PCoA plots with Bray–Curtis, Jensen–Shannon divergence, unweighted UniFrac, and weighted UniFrac distance measures for stool samples. Compressed OTU file with all the samples at Family level: stoolAndMeconiumOTUByFamily.csv.zip. Compressed OTU file with all the samples at Genus level: stoolAndMeconiumOTUByGenus.csv.zip. (ZIP 3263 kb

Demographic information of participants.

<p>Demographic information of participants.</p

Factors associated with the number of sexual partners by multiple linear regressions (length of time using dating apps).

<p>Factors associated with the number of sexual partners by multiple linear regressions (length of time using dating apps).</p

Correlates of consistent condom use by multiple logistic regressions.

<p>Correlates of consistent condom use by multiple logistic regressions.</p

Correlates of condom use at the last sexual intercourse by multiple logistic regressions.

<p>Correlates of condom use at the last sexual intercourse by multiple logistic regressions.</p

Mean changes from baseline in score on the 17-item Hamilton Rating Scale for Depression (HAMD-17), Clinical Global Impression-Severity (CGI-S) and Self-rating Depression Scale (SDS).

<p>Data are expressed as mean with 95% confidence interval (95% CI). * <i>P</i>≤0.037: between-group comparison using Student <i>t</i>-test.</p

Changes in score on depression scales from baseline in MDD patients.

a<p>Overall and between-group <i>P</i> values were obtained from linear mixed-effects model analysis and student <i>t</i>-test, respectively.</p><p>MDD, major depressive disorder; n-EA, noninvasive electroacupuncture; DCEAS, dense cranial electroacupuncture stimulation; 95% CI, 95% confidence interval; HAMD-17, 17-item Hamilton Rating Scale for Depression; CGI-S, Clinical Global Impression-Severity; SDS, Self-rating Depression Scale.</p

Adverse events occurred in at least 5% of patients in either group.

a<p><i>P</i> values were calculated from Fisher Exact test.</p><p>n-EA, noninvasive electroacupuncture; DCEAS, dense cranial electroacupuncture stimulation.</p