Infection of differentiated genetically modified cell lines with <i>P</i>. <i>berghei</i>.

<p>A) Infection of Slc4a1-/- and GYPC-/- differentiated cells. Labelling with MSP-1 and confocal microscopy showing parasites in the cells. B) Flow cytometric quantification of m-cherry-parasite positive cells at 6 and 24 hours. The plots show the populations distribution for an example experiment and the bar charts are the result of at least three separate experiments (* P<0.01 and ** P>0.1 comparing the clones at each time point with WT). Data are presented as mean +/-SD. See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158238#pone.0158238.s006" target="_blank">S6 Fig</a>.</p

Differentiation of JM8 mESCs towards erythropoiesis.

<p>A) <b>top panel</b>: pluripotent JM8 cell growth under light microscopy; <b>middle panel</b>: analysis of the indicated cell surface markers by flow cytometry. B) <b>top panel</b>: morphology of embryoid bodies under light microscopy; <b>bottom panel</b>: analysis of haematopoietic markers by qRT-PCR. C) <b>top panel</b>: differentiated erythroid cells under light microscopy, black arrow indicates haemoglobinised colony of cells; <b>middle panel</b>: analysis of cell surface markers by flow cytometry; <b>bottom panel</b>: haematopoietic proteins by qRT-PCR. (Data are presented as mean +/-SD).</p

Invasion of <i>in vitro</i> differentiated erythroid cells by <i>P</i>. <i>berghei</i>.

<p>A) Rapid Romanowski staining of infected red blood cells and mESC-derived erythroid cells 6 h and 24 h post-infection. B) Confocal microscopy of mouse erythrocytes and <i>in vitro</i> generated erythroid cells infected with <i>P</i>. <i>berghei</i>. Parasites are labelled with an anti-MSP1 antibody followed by a AF488-conjugated secondary antibody and nuclei are stained with DAPI. Invasion is followed at 6 and 24 hours. C) Flow cytometry quantification of infected cells by detecting mCherry-expressing parasites. The plots show the populations distribution for an example experiment and the bar charts are the result of at least three separate experiments. Data are presented at mean +/-SD.</p

Eyrthropoietic differentiation of genetically modified cell lines.

<p>A) Flow cytometric quantification of haematopoietic markers in the differentiated GM lines. B) qRT-PCR quantification of haematopoietic protein transcripts in differentiated GM lines. Data are presented as mean +/-SD. See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158238#pone.0158238.s001" target="_blank">S1 Fig</a>.</p

Analysis of differentiated JM8 erythroid cells.

<p>A) Rapid Romanowski stain of mouse blood and differentiated cells. B) Staining Haemoglobin in mouse blood and differentiated cells with o-dianisidine. C) Flow cytometry detection of nuclei in blood and differentiated cells labelled with Hoechst 33342.</p