Phylogenetic tree of representative sequences from dominant OTUs that fell in the genus <i>Vibrio</i> constructed using neighbor-joining method.

<p>Sequences from this study are shown in blue (site SETAS) and green (site D001). Sampling depths are indicated in brackets. Square indicates ¹³C-<i>D</i>-GlcN supplemented incubation. The topology of the phylogenetic tree was evaluated by bootstrap re-sampling method with 1,000 replicates, and bootstrap values greater than 50% are shown.</p

Distribution of DNA in a CsCl density gradient, produced from surface water of site D001 after incubation with supplemental ¹³C-labeled <i>D</i>-GlcN.

<p>Distribution of corresponding natural DNA shown as a negative control. Mixture of ¹²C- and ¹³C-DNA from <i>Escherichia coli</i> shown as a positive standard.</p

Taxa with relative abundances of >0.1% of total sequences in ¹³C-DNA-based (left axis) and ¹²C-DNA-based (right axis) communities at site D001.

<p>Taxa with relative abundances of >0.1% of total sequences in ¹³C-DNA-based (left axis) and ¹²C-DNA-based (right axis) communities at site D001.</p

Phylogenetic tree of representative sequences from dominant OTUs that fell in the <i>Roseobacter</i> clade constructed using neighbor-joining method.

<p>Sequences from this study are shown in blue (site SETAS) and green (site D001). Sampling depths are indicated in brackets. Square indicates ¹³C-<i>D</i>-GlcN supplemented incubation. Circle indicates ¹³C-<i>D</i>-Glc supplemented incubation. The topology of the phylogenetic tree was evaluated by bootstrap re-sampling method with 1,000 replicates, and bootstrap values greater than 50% are shown.</p

The 2^nd (heavy) to 11^th (light) density gradient fractions were analyzed with T-RFLP among 12 density gradient fractions produced from ultracentrifugation of DNA from surface water of site D001 after incubation with supplemental ¹³C-labeled <i>D</i>-GlcN.

<p>(a) Distribution of DNA concentrations in CsCl density gradients; (b) Relative abundance of T-RFLP peaks (color bars) in each DNA fraction; (c) Community similarity analysis based on T-RFLP fingerprinting.</p

Dendrogram constructed using group average model based on Bray-Curtis similarities between communities at D001 (a) and SEATS (b) sites.

<p>Dendrogram constructed using group average model based on Bray-Curtis similarities between communities at D001 (a) and SEATS (b) sites.</p