5 research outputs found
Ratiometric Fluorescence Aptasensor of Allergen Protein Based on Multivalent Aptamer-Encoded DNA Flowers as Fluorescence Resonance Energy Transfer Platform
Life-threatening allergic reactions to food allergens,
particularly
peanut protein Ara h1, are a growing public health concern affecting
millions of people worldwide. Thus, accurate and rapid detection is
necessary for allergen labeling and dietary guidance and ultimately
preventing allergic incidents. Herein, we present a novel ratiometric
fluorescence aptasensor based on multivalent aptamer-encoded DNA flowers
(Mul-DNFs) for the high-stability and sensitive detection of allergen
Ara h1. The flower-shaped Mul-DNFs were spontaneously packaged using
ultralong polymeric DNA amplicons driven by a rolling circle amplification
reaction, which contains a large number of Ara h1 specific recognition
units and has excellent binding properties. Furthermore, dual-color
fluorescence-labeled Mul-DNFs probes were developed by hybridizing
them with Cy3- and Cy5-labeled complementary DNA (cDNA) to serve as
a ratiometric fluorescence aptasensor platform based on fluorescence
resonance energy transfer. Benefiting from the combined merits of
the extraordinary synergistic multivalent binding ability of Mul-DNFs,
the excellent specificity of the aptamer, and the sensitivity of the
ratiometric sensor to avoid exogenous interference. The developed
ratiometric aptasensor showed excellent linearity (0.05–2000
ng mL–1) with a limit of detection of 0.02 ng mL–1. Additionally, the developed ratiometric fluorescence
aptasensor was utilized for quantifying the presence of Ara h1 in
milk, infant milk powder, cookies, bread, and chocolate with recoveries
of 95.7–106.3%. The proposed ratiometric aptasensor is expected
to be a prospective universal aptasensor platform for the rapid, sensitive,
and accurate determination of food and environmental hazards
MS<sup>3</sup> Imaging Enables the Simultaneous Analysis of Phospholipid CC and <i>sn</i>-Position Isomers in Tissues
Mass spectrometry (MS) imaging of lipids in tissues with
high structure
specificity is challenging in the effective fragmentation of position-selective
structures and the sensitive detection of multiple lipid isomers.
Herein, we develop an MS3 imaging method for the simultaneous
analysis of phospholipid CC and sn-position
isomers by on-tissue photochemical derivatization, nanospray desorption
electrospray ionization (nano-DESI), and a dual-linear ion trap MS
system. A novel laser-based sensing probe is developed for the real-time
adjustment of the probe-to-surface distance for nano-DESI. This method
is validated in mouse brain and kidney sections, showing its capability
of sensitive resolving and imaging of the fatty acyl chain composition,
the sn-position, and the CC location of phospholipids
in an MS3 scan. MS3 imaging of phospholipids
has shown the capability of differentiation of cancerous, fibrosis,
and adjacent normal regions in liver cancer tissues
Improved Sb<sub>2</sub>S<sub>3</sub>/TiO<sub>2</sub> Nanoarray Heterojunction Solar Cells by an Insulating Hole-Selective Tunneling Layer
Photovoltaic technology for sustainable
energy production
on a
large scale is mainly limited by the lack of solar cells with efficient,
stable, and low-cost characteristics. Antimony trisulfide (Sb2S3) has attracted intensive attention as a potential
photon-absorbing material for efficient and stable inorganic heterojunction
solar cells due to its feasible preparation. Here, a hole-selective
tunneling interfacial engineering method is developed to improve the
performance of solar cells based on solution-processed Sb2S3/TiO2 nanoarray heterojunction (Sb2S3/TiO2-NHJ), for which an ultrathin poly(methyl
methacrylate) (PMMA) film deposited into Sb2S3/TiO2-NHJ serves as the insulating hole-selective tunneling
interfacial layer in the solar cells. It is found that the hole-selective
tunneling PMMA interfacial layer effectively blocks the channels for
interfacial recombination of photogenerated charge carriers, and the
origins for the PMMA interfacial layer to boost the overall device
performance get elucidated
Development of a Miniature Mass Spectrometry System for Point-of-Care Analysis of Lipid Isomers Based on Ozone-Induced Dissociation
Disorder of lipid homeostasis is closely associated with
a variety
of diseases. Although mass spectrometry (MS) approaches have been
well developed for the characterization of lipids, it still lacks
an integrated and compact MS system that is capable of rapid and detailed
lipid structural characterization and can be conveniently transferred
into different laboratories. In this work, we describe a novel miniature
MS system with the capability of both ozone-induced dissociation (OzID)
and collision-induced dissociation (CID) for the assignment of sites
of unsaturation and sn-positions in glycerolipids.
A miniature ozone generator was developed, which can be operated at
a relatively high pressure. By maintaining high-concentration ozone
inside the linear ion trap, OzID efficiency was significantly improved
for the identification of CC locations in unsaturated lipids,
with reaction times as short as 10 ms. Finally, the miniature OzID
MS system was applied to the analysis of CC locations and sn-positions of lipids from biological samples. Direct sampling
and fast detection of changes in phospholipid isomers were demonstrated
for the rapid discrimination of breast cancer tissue samples, showing
the potential of the miniature OzID MS system for point-of-care analysis
of lipid isomer biomarkers in complex samples
Data_Sheet_1_Influence of pre-stroke dependency on safety and efficacy of endovascular therapy: A systematic review and meta-analysis.PDF
Background and purposeIn the landmark trials studying endovascular thrombectomy (EVT), pre-stroke dependent (PSD) patients were generally excluded. This systematic review and meta-analysis aimed to compare the safety and efficacy of EVT between PSD and pre-stroke independent (PSI) patients.MethodsWe searched CENTRAL, Embase, and Ovid MEDLINE up to 11 November 2021 for studies assessing PSD and PSI patients, which were separately defined as pre-stroke mRS score >2 or >1, and ≤2 or ≤1 accordingly. Two authors extracted data and assessed the risk of bias. A meta-analysis was carried out using the random-effects model. Adjusted OR and 95% CI were used to estimate adjusted pool effects. The main outcomes included favorable outcomes, successful recanalization, symptomatic intracranial hemorrhage, and 90-day mortality.ResultsA total of 8,004 records met the initial search strategy, and ten studies were included in the final decision. Compared with PSImRS≤2, PSDmRS>2 had a lower favorable outcome (OR 0.51; 95% CI, 0.33–0.79) and higher 90-day mortality (OR 3.32; 95% CI, 2.77–3.98). No significant difference was found in successful recanalization and sICH. After adjustment, only 90-day mortality (aOR 1.99; 95% CI, 1.58–2.49) remained significantly higher in PSDmRS>2. Compared with PSImRS≤1, PSDmRS>1 had lower 90-day mortality (OR, 3.10; 95% CI, 1.84–5.24). No significant difference was found regarding the favorable outcome, successful recanalization, and sICH. After adjustment, no significant difference was found in a favorable outcome, but a higher rate of 90-day mortality (aOR, 2.13; 95% CI, 1.66–2.72) remained in PSDmRS>1.ConclusionsPSD does not innately influence the EVT outcomes regarding sICH and favorable outcomes but may increase the risk of 90-day mortality. Until further evidence is available, it is reasonable to suggest EVT for patients with PSD.</p