Additional file 6: of Oral administration of a select mixture of Bacillus probiotics generates Tr1 cells in weaned F4ab/acR− pigs challenged with an F4+ ETEC/VTEC/EPEC strain

Immunofluorescence staining of F4 + ETEC/VTEC/EPEC strain in the ileum. The figures show representative photomicrographs of F4+ ETEC/VTEC/EPEC (red) adhesion to the ileal mucosa of pigs 1 week after F4+ ETEC/VTEC/EPEC challenge. The typical features associated with an attachment rating were as follows: 0 (A), no observed attachment of F4+ ETEC/VTEC/EPEC to the ileal mucosa; 1 (B); 2 (C); 3 (D); and 4 (E), F4+ ETEC/VTEC/EPEC were adhered to the crypt and entire villus. Scale bars, 50 μm

Additional file 1: of Oral administration of a select mixture of Bacillus probiotics generates Tr1 cells in weaned F4ab/acR− pigs challenged with an F4+ ETEC/VTEC/EPEC strain

Sequences of oligonucleotide primers used for real-time PCR, length of the respective PCR product and gene accession number. The table shows the sequences of primers used for real-time PCR in this study

Additional file 2: of Oral administration of a select mixture of Bacillus probiotics generates Tr1 cells in weaned F4ab/acR− pigs challenged with an F4+ ETEC/VTEC/EPEC strain

Sequences of oligonucleotide primers used for quantitative real-time PCR, length of the respective PCR product and gene accession number. The table shows the sequences of primers used for quantitative real-time PCR in this study