Phylogram of <i>Ptilagrostis</i> obtained from MrBayes analysis of ITS dataset.

<p>Numbers above branches are support values of ML, numbers below the branches are support values of BI.</p

Illustration of <i>Ptilagrostis contracta</i>.

<p>A. habit; B. glume; C. floret; D. palea; E. callus F. outer surface of ligule; G. inner surface of ligule. Scale bar in B, C, F, G represent 5 mm; in D represent 2 mm; in E represent 0.5 mm (Drawn by Z. S. Zhang).</p

<i>Ptilagrostis contracta</i> (Stipeae, Poaceae), a New Species Endemic to Qinghai-Tibet Plateau - Sheet 0

<i>Ptilagrostis contracta</i> (Stipeae, Poaceae), a New Species Endemic to Qinghai-Tibet Plateau - Sheet

<i>Ptilagrostis contracta</i>.

<p>A-B. habitat; C. contracted panicle; D. base of panicle branch (pulvinae absent); E. spikelets; F. floret (lemma evenly pubescent); G. chromosomes; H. karyotype; I. Lemma epidermal pattern. Scale bar in D represents 2 mm; in E, F represent 5 mm; in G, H represent 5 μm.</p

RecN focus is close to the center of the cell treated with MMC.

<p>(A) Most RecN-GFP foci are colocalized with nucleoid at the center area of cells when treated with 2 μg/mL MMC. (B, C) RecN-GFP foci and nucleoids were colocalized at the position of cell plate between two daughter cells in dividing cell pairs. (D) RecN-GFP focus was localized in one of the daughter cells when the chromosome was segregated at the later period of cell cycle after MMC treatment. (E) The white arrows indicated that in a few cells, RecN–GFP foci were not colocalized with the chromosome at the central areas (5% in total, n = 196). (F) Nucleoids were more compacted and the number of RecN-GFP foci increased in some cells (white arrows) (21% of cell in a total, n = 213) after the treatment with 4 μg/mL MMC. Photographs were taken by using an Olympus FV1000 confocal microscope. Cells were stained with DAPI (blue). Each photograph involved 3 different channels for DAPI (in blue) GFP (in green) fluorescence and bright field. Scale bars correspond to 1 μm.</p

RecN appears as a single discrete globular focus in newly formed heterocyst and disappears in mature ones.

<p>(A) RecN-GFP focus in heterocyst (white arrow). (B) Rates (in percentage) of heterocysts with RecN–GFP foci after the starvation of combined nitrogen. (C) Western blotting analysis of protein extracts from vegetative cells or enriched heterocysts using anti-RecN antiserum. Lanes 1 and 2: extracts from vegetative cell in 0 and 12 h after nitrogen deprivation; Lanes 3, 4 and 5: extracts from heterocyst in 24, 48 and 72 h after nitrogen deprivation. (D) High concentration of MMC did not lead to the reformation of RecN-GFP foci in mature heterocysts. After 24 h deprivation of combined nitrogen, 4 μg/mL MMC was added into the medium for 3-day cultivation and then the photographs were captured. The white arrows indicate heterocysts. Photographs were taken by using an Olympus FV1000 confocal microscope. Cells were stained with DAPI (blue). The red fluorescence is the fluorescence of photosynthetic pigment. Scale bars correspond to 1 μm.</p

Movement of RecN in vegetative cells displayed by time-lapse imaging.

<p>The dynamic localization of RecN focus is demonstrated by time-lapse microscopy from the RG-W. Cells were photographed at 3–4 h intervals. Images on the right were taken in bright field and those on the left were taken in fluorescence respectively in 0, 3, 6, and 10 h. The newborn foci were also marked. Images were taken using a Nikon Eclipse 80i microscope. Scale bars correspond to 1 μm.</p

Segregation of RecN foci during vegetative cell division.

<p>(A) The method used to quantify the relative distance between foci along the cell division axis. In the formula, “a” means a cell inheriting the original RecN focus after division; “b” means a cell with both an original and a newborn RecN focus. Photographs were captured by using an Olympus FV1000 confocal microscope. Cells were stained with DAPI (blue). Scale bars correspond to 1 μm. (B) Cell number distribution obtained according to the relative distance. (C) Original and newborn RecN foci usually were nearly symmetrically distributed along the cell division axis. Photograph by using a Nikon Eclipse 80i microscope, in the fluorescence and bright fields. Scale bars correspond to 1 μm.</p

RecN forms a single discrete globular focus in vegetative cells.

<p>(A) The three types of vegetative cells divided according to the cell division stages. (B) Localization of RecN-GFP was determined according to its relative position along the x axis and y axis. (C). The locations of the foci in the three types of vegetative cells. The coordinate 0 is the center of the cell. (D) The distributions (in percentage) of the three types of vegetative cells at the relative position of the X-axis. The dynamic localization of RecN-GFP foci was observed with an Olympus FV1000 confocal microscope. Cells were stained with DAPI (blue). Scale bars correspond to 1 μm.</p

ReHo mapping of BD (under depressive state) patients (compared to UD patients) shows a significantly increase in ReHo in the thalamus.

<p>Hot and cold colors indicate the increase and decrease of ReHo, respectively.</p