1 research outputs found
Watsonianone A from Rhodomyrtus tomentosa Fruit Attenuates Respiratory-Syncytial-Virus-Induced Inflammation <i>In Vitro</i>
Respiratory
syncytial virus (RSV) is one of the most common respiratory pathogens.
Immoderate inflammation plays a great role in causing RSV-induced
diseases. In the present study, watsonianone A, isolated from the
fruit of Rhodomyrtus tomentosa (Ait.)
Hassk, was found to show a good inhibitory effect on RSV-induced NO
production, with a half-maximal inhibitory concentration of 37.2 ±
1.6 μM. Enzyme-linked immunosorbent assay and fluorescence quantitative
polymerase chain reaction analyses indicated that watsonianone A markedly
reduced both mRNA and protein levels of tumor necrosis factor α,
interleukin 6, and monocyte chemoattractant protein 1 in RSV-infected
RAW264.7 cells. Mechanistically, watsonianone A inhibited nuclear
factor κB (NF-κB) activation by suppressing IκBα
phosphorylation. Further analysis revealed that watsonianone A activated
the thioredoxin system and decreased intracellular reactive oxygen
species (ROS) levels, which are closely associated with NF-κB
activation in RSV-infected cells. These results reveal that watsonianone
A can attenuate RSV-induced inflammation via the suppression of ROS-sensitive
inflammatory signaling