Who is leading China's family planning policy discourse in Weibo? A social media text mining analysis

The advent of digital social media in China has altered our understanding of who sets the policy agenda and forms public opinion. Using text mining analysis of more than 74,000 Weibo user comments (over 4 million words) on 6 years' worth of The People's Daily media coverage, this study investigates social media interactions on family planning policy issues between the state-run news media and individual users in China. Our analysis demonstrates that Weibo postings about the topic by government-run news networks and comments by the general public are affecting each other, but also presenting partially reverse or bottom-up agenda-setting effects. Through latent dirichlet allocation (LDA) modeling, we identified major latent topic sets (women's right to work, family culture/tradition, law/regulation, and social welfare/wellbeing) and found that Weibo users' main concerns on China's family planning have changed over time. We also found that gender differences affect the topics of commenters

Additional file 1: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Table S1. Risk of bias in included studies using modified Newcastle-Ottawa Scale. (DOCX 51 kb

Additional file 4: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S3. Forest plot and meta-analysis of postoperative long-term eGFR. (PDF 160 kb

Additional file 5: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S4. Forest plot and meta-analysis of postoperative acute renal failure. (PDF 159 kb

Additional file 11: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S10. Forest plot and subgroup meta-analysis of postoperative long-term change of Cr level. (PDF 83 kb

Direct comparisons between phenotype of THP-1, N₂ and Nef.

<p>Surface expressions of CD11c, CD14, CD40,CD80,CD83,CD86,HLA-DR of stable cell lines–Nef and N₂ (control) within 4 weeks were determined via flow cytometry following establishment. The histograms showed a direct comparison of CD11c,CD14, CD40,CD80,CD83,CD86,HLA-DR levels for Nef <i>vs</i> N₂ and THP-1 Blue line, Nef; green line, N2; red line, THP-1. One representative of three performed experiments was presented.</p

Establishment of stable cell lines Nef and N₂.

<p>The THP-1 cell was transfected with pEGFP-<i>nef</i> and pEGFP-N₂ respectively, giving rise to Nef and N₂ following the limiting dilution in the presence of 550 µg/ml of G418 within 4 weeks. (A): The visualized PCR product on an ethidium bromide stained 1.2% agarose gel, without any template as controls; (B): a, THP-1 in light background; b, THP-1 in black background; c, EGFP expression by N₂ in light background; d, EGFP expression by N₂ in black background; e, Nef-EGFP expression by Nef in light background; f, Nef-EGFP expression by Nef in black background. Microscopy of cells in culture at 400X.magnification.</p

Comparative morphology and phenotype between THP-1, N₂ and Nef upon co-stimulation with GM-CSF,IL-4,TNFα and ionomycin.

<p>(A): morphology of the above cell types was observed in light background (upper panel) and black one (lower panel) at magnification of 400X; (B): The histograms showed a direct comparison of CD11c, CD14, CD40,CD80,CD83,CD86,HLA-DR levels for Nef <i>vs</i> N₂ and THP-1,(7-day culture based on “5+2” protocol ); (C): The histograms showed a direct comparison of above surface markers expression levels for Nef <i>vs</i> N₂ and THP-1 (2-day culture based on “0+2” protocol). One representative of three performed experiments was presented.</p

Additional file 6: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S5. Forest plot and meta-analysis of postoperative newly increased chronic kidney disease (CKD)(stage≥3). (PDF 82 kb

Additional file 10: of Off-clamp partial nephrectomy has a positive impact on short- and long-term renal function: a systematic review and meta-analysis

Figure S9. Forest plot and subgroup meta-analysis of postoperative long-term % eGFR change. (PDF 84 kb