Detoxifying symbionts in agriculturally important pest insects

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Fortunate those that are starting now

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The microbial diversity of cabbage pest Delia radicum across multiple life stages

The cabbage root fly Delia radicum is a worldwide pest that causes yield losses of many common cabbage crops. The bacteria associated with D. radicum are suggested to influence the pest status of their host. In this study, we characterized insect-associated bacteria of Delia radicum across multiple life stages and of their diet plant (turnip, Brassica rapa subsp. rapa) by sequencing the V3-V4 region of 16S rRNA genes using the Illumina MiSeq platform. In total, over 1.2M paired-end reads were obtained, identifying 1006 bacterial amplicon sequence variants (ASVs) in samples obtained from the eggs, larvae, pupae and adults of Delia radicum, as well as turnips that were either fresh or infested with D. radicum larvae. The microbial community in Delia radicum was dominated by Wolbachia, a common endosymbiont of arthropods which we found in all of the investigated insect samples, with the pupal stage having the highest relative abundance. Moderate amounts of Firmicutes were found only in adult D. radicum flies, but not in previous life stages. Actinobacteria were mostly found on the eggs and on the skin of fresh plants on which the eggs were deposited. These plants also harbored a large amount of Pseudomonas. The bacterial diversity of the healthy turnip was low, whereas the microbial community of decaying turnips that were heavily infested by D. radicum larvae and showing symptoms of advanced soft rot was characterized by a high bacterial diversity. Taken together, this work provides insights into the bacterial communities associated with the cabbage pest Delia radicum and its associated disease symptoms

Experimental evidence of an acetate transporter protein and characterization of acetate activation in aceticlastic methanogenesis of Methanosarcina mazei

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Effect of concentration and hydraulic reaction time on the removal of pharmaceutical compounds in a membrane bioreactor inoculated with activated sludge

We evaluated the effect of pharmaceutical concentration and hydraulic retention time in removal rates and efficiency. We also looked at the microbial community changes in response to higher pharmaceutical concentrations. To do that, we inoculated a membrane bioreactor with activated sludge and fed it with ammonium, acetate, and methanol as main growth substrates. We increased pharmaceutical concentration over time and also hydraulic retention time

Evidence for the involvement of two heterodisulfide reductases in the energy-conserving system of Methanomassiliicoccus luminyensis

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Roles of Thermokarst Lakes in a Warming World

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