Eighteenth Year of the Gulf of Maine Environmental Monitoring Program

This report summarizes the metals and organic contaminant data associated with the collection and analyses of blue mussel (Mytilus edulis) tissue from selected sites along the Gulf of Maine coast during the 2008 sampling season. Contaminant monitoring is conducted by the Gulfwatch Program for the Gulf of Maine Council on the Marine Environment (GOMC). A subset of these data is compared with analytical results from earlier Gulfwatch monitoring (2001-2007). Statistical analyses are limited to descriptive measures of replicates from selected sampling sites and include: arithmetic means, and appropriate measures of variance. The primary purpose of this report is to present the current annual results, present graphical representation of spatial and temporal trends and identify potential outliers in order to provide investigators and other interested persons with contemporary information concerning water quality in the Gulf of Maine, as reflected by uptake into resident shellfish (mussels and clams)

Data for 'Something in the way she moves': the functional significance of flexibility in the multiple roles of protein disulfide isomerase (PDI)

Protein disulfide isomerase (PDI) has diverse functions in the endoplasmic reticulum as catalyst of redox transfer, disulfide isomerization and oxidative protein folding, as molecular chaperone and in multi-subunit complexes. It interacts with an extraordinarily wide range of substrate and partner proteins, but there is only limited structural information on these interactions. Extensive evidence on the flexibility of PDI in solution is not matched by any detailed picture of the scope of its motion. A new rapid method for simulating the motion of large proteins provides detailed molecular trajectories for PDI demonstrating extensive changes in the relative orientation of its four domains, great variation in the distances between key sites and internal motion within the core ligand-binding domain. The review shows that these simulations are consistent with experimental evidence and provide insight into the functional capabilities conferred by the extensive flexible motion of PDI

A Targeted Sampling Strategy for Compressive Cryo Focused Ion Beam Scanning Electron Microscopy

Cryo Focused Ion-Beam Scanning Electron Microscopy (cryo FIB-SEM) enables three-dimensional and nanoscale imaging of biological specimens via a slice and view mechanism. The FIB-SEM experiments are, however, limited by a slow (typically, several hours) acquisition process and the high electron doses imposed on the beam sensitive specimen can cause damage. In this work, we present a compressive sensing variant of cryo FIB-SEM capable of reducing the operational electron dose and increasing speed. We propose two Targeted Sampling (TS) strategies that leverage the reconstructed image of the previous sample layer as a prior for designing the next subsampling mask. Our image recovery is based on a blind Bayesian dictionary learning approach, i.e., Beta Process Factor Analysis (BPFA). This method is experimentally viable due to our ultra-fast GPU-based implementation of BPFA. Simulations on artificial compressive FIB-SEM measurements validate the success of proposed methods: the operational electron dose can be reduced by up to 20 times. These methods have large implications for the cryo FIB-SEM community, in which the imaging of beam sensitive biological materials without beam damage is crucial.Comment: Submitted to ICASSP 202

Abelian D-terms and the superpartner spectrum of anomaly-mediated supersymmetry breaking

We address the tachyonic slepton problem of anomaly mediated supersymmetry breaking using abelian D-terms. We demonstrate that the most general extra U(1) symmetry that does not disrupt gauge coupling unification has a large set of possible charges that solves the problem. It is shown that previous studies in this direction that added both an extra hypercharge D-term and another D-term induced by B-L symmetry (or similar) can be mapped into a single D-term of the general ancillary U(1)_a. The U(1)_a formalism enables identifying the sign of squark mass corrections which leads to an upper bound of the entire superpartner spectrum given knowledge of just one superpartner mass.Comment: 10 pages, 2 figures, [v2] reference added, [v3] Eq. (9) corrected, results unaffected, [v4] version to be published in Phys. Rev. D, expanded parameter space for figures to match tex

The Potential of Subsampling and Inpainting for Fast Low-Dose Cryo FIB-SEM Imaging and Tomography

Traditional image acquisition for cryo focused ion-beam scanning electron microscopy tomography often sees thousands of images being captured over a period of many hours, with immense data sets being produced. When imaging beam sensitive materials, these images are often compromised by additional constraints related to beam damage and the devitrification of the material during imaging, which renders data acquisition both costly and unreliable. Subsampling and inpainting are proposed as solutions for both of these aspects, allowing fast and low-dose imaging to take place in the FIB-SEM without an appreciable low in image quality. In this work, experimental data is presented which validates subsampling and inpainting as a useful tool for convenient and reliable data acquisition in a FIB-SEM, with new methods of handling 3-dimensional data being employed in context of dictionary learning and inpainting algorithms using a newly developed microscope control software and data recovery algorithm.Comment: In submission to "Microscopy and Microanalysis" journal. Authorship reviewed from previous submissio

Changes in cardiac-driven perivascular fluid movement around the MCA in a pharmacological model of acute hypertension detected with non-invasive MRI

Perivascular spaces mediate a complex interaction between cerebrospinal fluid and brain tissue that may be an important pathway for solute waste clearance. Their structural or functional derangement may contribute to the development of age-related neurogenerative conditions. Here, we employed a non-invasive low b-value diffusion-weighted ECG-gated MRI method to capture perivascular fluid movement around the middle cerebral artery of the anaesthetised rat brain. Using this method, we show that such MRI estimates of perivascular fluid movement directionality are highly sensitive to the cardiac cycle. We then show that these measures of fluid movement directionality are decreased in the angiotensin-II pharmacological model of acute hypertension, with an associated dampening of vessel pulsatility. This translational MRI method may, therefore, be useful to monitor derangement of perivascular fluid movement associated with cardiovascular pathologies, such as hypertension, in order to further our understanding of perivascular function in neurology

Volumetric Spatial Correlations of Neurovascular Coupling Studied using Single Pulse Opto-fMRI

Neurovascular coupling describes the link between neuronal activity and cerebral blood flow. This relationship has been the subject of intense scrutiny, with most previous work seeking to understand temporal correlations that describe neurovascular coupling. However, to date, the study of spatial correlations has been limited to two-dimensional mapping of neuronal or vascular derived signals emanating from the brain's surface, using optical imaging techniques. Here, we investigate spatial correlations of neurovascular coupling in three dimensions, by applying a single 10 ms pulse of light to trigger optogenetic activation of cortical neurons transduced to express channelrhodopsin2, with concurrent fMRI. We estimated the spatial extent of increased neuronal activity using a model that takes into the account the scattering and absorption of blue light in brain tissue together with the relative density of channelrhodopsin2 expression across cortical layers. This method allows precise modulation of the volume of activated tissue in the cerebral cortex with concurrent three-dimensional mapping of functional hyperemia. Single pulse opto-fMRI minimizes adaptation, avoids heating artefacts and enables confined recruitment of the neuronal activity. Using this novel method, we present evidence for direct proportionality of volumetric spatial neurovascular coupling in the cerebral cortex

Sticky DNA, a Long GAA·GAA·TTC Triplex That Is Formed Intramolecularly, in the Sequence of Intron 1 of the Frataxin Gene

Friedreich's ataxia is caused by the massive expansion of GAA.TTC repeats in intron 1 of the frataxin (X25) gene. Our prior investigations showed that long GAA.TTC repeats formed very stable triplex structures which caused two repeat tracts to adhere to each other (sticky DNA). This process was dependent on negative supercoiling and the presence of divalent metal ions. Herein, we have investigated the formation of sticky DNA from plasmid monomers and dimers; sticky DNA is formed only when two tracts of sufficiently long (GAA.TTC)(n) (n = 59-270) are present in a single plasmid DNA and are in the direct repeat orientation. If the inserts are in the indirect (inverted) repeat orientation, no sticky DNA was observed. Furthermore, kinetic studies support the intramolecular nature of sticky DNA formation. Electron microscopy investigations also provide strong data for sticky DNA as a single long triplex. Hence, these results give new insights into our understanding of the capacity of sticky DNA to inhibit transcription and thereby reduce the level of frataxin protein as related to the etiology of Friedreich's ataxia

Higgs boson mass limits in perturbative unification theories

Motivated in part by recent demonstrations that electroweak unification into a simple group may occur at a low scale, we detail the requirements on the Higgs mass if the unification is to be perturbative. We do this for the Standard Model effective theory, minimal supersymmetry, and next-to-minimal supersymmetry with an additional singlet field. Within the Standard Model framework, we find that perturbative unification with sin2(thetaW)=1/4 occurs at Lambda=3.8 TeV and requires mh<460 GeV, whereas perturbative unification with sin2(thetaW)=3/8 requires mh<200 GeV. In supersymmetry, the presentation of the Higgs mass predictions can be significantly simplified, yet remain meaningful, by using a single supersymmetry breaking parameter Delta_S. We present Higgs mass limits in terms of Delta_S for the minimal supersymmetric model and the next-to-minimal supersymmetric model. We show that in next-to-minimal supersymmetry, the Higgs mass upper limit can be as large as 500 GeV even for moderate supersymmetry masses if the perturbative unification scale is low (e.g., Lambda=10 TeV).Comment: 20 pages, latex, 6 figures, references adde