MOESM1 of Natural genetic variability reduces recalcitrance in poplar

Additional file 1. Additional details for rare natural poplar variants. Additional file 1 provides the list of plants and their lignin content. This file also provides details of the graphs with box plots, and average imprecisions in measurement of sugar yield and lignin content

Additional file 5: Table S3. of Quantitative trait locus mapping of Populus bark features and stem diameter

Number of candidate genes detected across QTL for the three traits. Note: The number of genes for each trait in QTL clusters based on MQM mapping with cofactor selection, sorted by significance and reproducibility. (DOCX 13 kb

Additional file 3: FigureS2. of Quantitative trait locus mapping of Populus bark features and stem diameter

Frequency distribution for bark texture, diameter and bark thickness (a, b, and c, respectively) across Oregon and West Virginia sites and various years in Populus Family 52–124. NOTE: All supporting tables, except for Table S3, are in excel format submitted as separate files. (TIFF 209 kb

Additional file 7: Table S5. of Quantitative trait locus mapping of Populus bark features and stem diameter

The 90th percentile candidate genes within the ninety four QTL detected in Populus Family 52–124. Physical localization, annotation and expression profile of gene models in the 90th percentile with high expression within LOD peaks for each QTL interval for all traits. (XLSX 78 kb

居民組織

2003-2004 > Academic research: refereed > Chapter in an edited book (author

Characterization of <i>MORE AXILLARY GROWTH</i> Genes in <i>Populus</i>

<div><p>Background</p><p>Strigolactones are a new class of plant hormones that play a key role in regulating shoot branching. Studies of branching mutants in Arabidopsis, pea, rice and petunia have identified several key genes involved in strigolactone biosynthesis or signaling pathway. In the model plant Arabidopsis, <i>MORE AXILLARY GROWTH1</i> (<i>MAX1</i>), <i>MAX2</i>, <i>MAX3</i> and <i>MAX4</i> are four founding members of strigolactone pathway genes. However, little is known about the strigolactone pathway genes in the woody perennial plants.</p><p>Methodology/Principal Finding</p><p>Here we report the identification of MAX homologues in the woody model plant <i>Populus trichocarpa</i>. We identified the sequence homologues for each MAX protein in <i>P. trichocarpa</i>. Gene expression analysis revealed that <i>Populus MAX</i> paralogous genes are differentially expressed across various tissues and organs. Furthermore, we showed that <i>Populus MAX</i> genes could complement or partially complement the shoot branching phenotypes of the corresponding Arabidopsis <i>max</i> mutants.</p><p>Conclusion/Significance</p><p>This study provides genetic evidence that strigolactone pathway genes are likely conserved in the woody perennial plants and lays a foundation for further characterization of strigolactone pathway and its functions in the woody perennial plants.</p></div

Expression of <i>Populus MAX</i> homologous genes across various tissues and organs.

<p>(<b>A</b>) Illustration of tissues and organs used for expression analysis. (<b>B</b>) Quantitative RT-PCR data. Shown are means ± S.E. of three biological replicates.</p

Genetic complementation of <i>Arabidopsis max1</i> mutants with <i>Populus MAX1</i> genes.

<p>(<b>A</b>) RT-PCR analysis of <i>35S:PtrMAX1a</i> transgenic lines. (<b>B</b>) RT-PCR analysis of <i>35S:PtrMAX1b</i> transgenic lines. (<b>C</b>) Number of primary rosette-leaf branches. Shown are average numbers of primary rosette-leaf branches from at least 10 individual plants ± S.E. *, significant difference from <i>max1-4</i>, p<0.05.</p

Genetic complementation of <i>Arabidopsis max2</i> mutants with <i>Populus MAX2</i> genes.

<p>(<b>A</b>) RT-PCR analysis of <i>35S:PtrMAX2a</i> transgenic lines. (<b>B</b>) RT-PCR analysis of <i>35S:PtrMAX2b</i> transgenic lines. (<b>C</b>) Number of primary rosette-leaf branches. Shown are average numbers of primary rosette-leaf branches from at least 10 individual plants ± S.E. *, significant difference from <i>max2-4</i>, p<0.05.</p

LOD traces for QTL B<i>M-2</i> on LG II based on 4-year height (red) and 4-year diameter (green) measured in Clatskanie and 4-year height measured in Boardman (purple).

<p>Broken horizontal line represents linkage groupwise LOD significance threshold calculated based on 1,000 permutations at the 0.05 significance level.</p