3 research outputs found
Within-Mice Comparison of Microdialysis and Fiber Photometry-Recorded Dopamine Biosensor during Amphetamine Response
A fundamental concept in neuroscience is the transmission
of information
between neurons via neurotransmitters, -modulators, and -peptides.
For the past decades, the gold standard for measuring neurochemicals
in awake animals has been microdialysis (MD). The emergence of genetically
encoded fluorescence-based biosensors, as well as in vivo optical techniques such as fiber photometry (FP), has introduced
technologically distinct means of measuring neurotransmission. To
directly compare MD and FP, we performed concurrent within-animal
recordings of extracellular dopamine (DA) in the dorsal striatum (DS)
before and after administration of amphetamine in awake, freely behaving
mice expressing the dopamine sensor dLight1.3b. We show that despite
temporal differences, MD- and FP-based readouts of DA correlate well
within mice. Down-sampling of FP data showed temporal correlation
to MD data, with less variance observed using FP. We also present
evidence that DA fluctuations periodically reach low levels, and naïve
animals have rapid, predrug DA dynamics measured with FP that correlate
to the subsequent pharmacodynamics of amphetamine as measured with
MD and FP