Measurement and Correlation of Solubility on Reactive Crystallization of Methyl D‑(−)-4-Hydroxy-phenylglycinate

In this paper, the solubility of methyl D-(−)-4-hydroxy-phenylglycinate was measured under the different pH levels, pure solvents, mixed solvents, ionic strength, and impurities in the temperature range from 283 to 323 about 5 K intervals by using laser method at atmospheric pressure. The results reveal that the solubility of methyl D-(−)-4-hydroxy-phenylglycinate increases with increasing temperature in all selected solvents, which decreases with increasing mole fraction composition of water in the mixed solvents. The solubility curve of methyl D-(−)-4-hydroxy-phenylglycinate in the aqueous solution at different pH is “U” shape and the solubility of methyl D-(−)-4-hydroxy-phenylglycinate increases with concentrations of ammonium chloride and has no obvious changes when its concentration increases up to 1.25 mol/kg. It is beneficial to maximize the reaction conversion rate of D-4-hydroxyphenylglycine methyl ester hydrochloride and reduce the residual D-4-hydroxyphenylglycine methyl ester hydrochloride on reactive crystallization of methyl D-(−)-4-hydroxy-phenylglycinate. Furthermore, the modified Apelblat equation and <i>C</i>_T = <i>C</i>₀(α_H⁺/<i>k</i>₁ + <i>k</i>₂/α_H⁺ + 1) type correlation regression model have made good correlation of the experimental solubility in pure solvents, mixed solvents, and the aqueous solution at different pH, respectively

Synthesis and biological evaluation of water-soluble <i>trans</i>-[bicyclo[2.2.2]octane-7<i>R</i>,8<i>R</i>-diamine]platinum(II) complexes with linear or branched alkoxyacetates as leaving groups

<p>Four platinum(II) complexes, <i>trans</i>-[bicyclo[2.2.2]octane-7<i>R</i>,8<i>R</i>-diamine]bis(alkoxyacetato-<i>O</i>,<i>O’</i>) platinum(II) (alkoxyacetate = methoxyacetate (<b>2</b>), ethoxyacetate (<b>3</b>), isopropoxyacetate (<b>4</b>), and <i>tert</i>-butoxyacetate (<b>5</b>)) were synthesized and spectrally characterized. The cytotoxicity of these water-soluble complexes was evaluated by CCK-8 assay <i>in vitro</i> against HCT-116, HepG-2, and A549 cancer cell lines. Most of the complexes had cytotoxic activity against the tested cancer cell lines. Among them, <b>3</b> showed more potent antitumor effect than cisplatin or oxaliplatin. Complex <b>3</b> could cause HCT-116 cell line death based on an apoptotic pathway since it has a dicyclic moiety similar to 1<i>R</i>,2<i>R</i>-diaminocyclohexane in oxaliplatin. Agarose gel electrophoresis on the interaction between <b>3</b> and DNA indicated that it has different behavior from that of cisplatin or oxaliplatin, which has a high correlation with the ligand used.</p

Antibacterial PVDF Coral-Like Hierarchical Structure Composite Film Fabrication for Self-Cleaning and Radiative Cooling Effect

Passive radiative cooling (PRC) is a zero-energy-consumption technology that reflects sunlight and radiates heat to cold outer space. In this work, a porous poly(vinylidene fluoride)–poly(methyl methacrylate) (PVDF–PMMA) composite film is fabricated by decorating zinc-imidazolate metal–organic framework (MOF) (ZIF-8) particles obtained by phase inversion. Due to the competent scattering via the coral-like hierarchical structures and the vibration excitations of specific functional groups, the prepared film exhibits good solar reflectance (92.6%) and intermediate infrared emittance (99.1%), with an average sub-ambient cooling of 10.4 °C under a solar radiation intensity of 0.6 AM1.5. Additionally, poly(vinylidene fluoride) has a low surface energy, while the ZIF-8 particles and coral-like hierarchical structures enhance the surface roughness, endowing the surface with significant superhydrophobicity characterized by a water contact angle (WCA) of 157.5° and a sliding angle (SA) of 2°. These films exhibit excellent antibacterial properties. When the content of ZIF-8 particles in the film is 300 mg·L–1, the antibacterial rate reaches 100% after 1 h of treatment. Thus, the ZIF-8 porous poly(vinylidene fluoride)–poly(methyl methacrylate) composite (ZPPP) film has potential application prospects in areas with high health and environmental requirements, such as cold chain transportation and public spaces

Antibacterial PVDF Coral-Like Hierarchical Structure Composite Film Fabrication for Self-Cleaning and Radiative Cooling Effect

Passive radiative cooling (PRC) is a zero-energy-consumption technology that reflects sunlight and radiates heat to cold outer space. In this work, a porous poly(vinylidene fluoride)–poly(methyl methacrylate) (PVDF–PMMA) composite film is fabricated by decorating zinc-imidazolate metal–organic framework (MOF) (ZIF-8) particles obtained by phase inversion. Due to the competent scattering via the coral-like hierarchical structures and the vibration excitations of specific functional groups, the prepared film exhibits good solar reflectance (92.6%) and intermediate infrared emittance (99.1%), with an average sub-ambient cooling of 10.4 °C under a solar radiation intensity of 0.6 AM1.5. Additionally, poly(vinylidene fluoride) has a low surface energy, while the ZIF-8 particles and coral-like hierarchical structures enhance the surface roughness, endowing the surface with significant superhydrophobicity characterized by a water contact angle (WCA) of 157.5° and a sliding angle (SA) of 2°. These films exhibit excellent antibacterial properties. When the content of ZIF-8 particles in the film is 300 mg·L–1, the antibacterial rate reaches 100% after 1 h of treatment. Thus, the ZIF-8 porous poly(vinylidene fluoride)–poly(methyl methacrylate) composite (ZPPP) film has potential application prospects in areas with high health and environmental requirements, such as cold chain transportation and public spaces

Additional file 1 of Outlier detection in spatial error models using modified thresholding-based iterative procedure for outlier detection approach

Supplementary Material 1

Antibacterial PVDF Coral-Like Hierarchical Structure Composite Film Fabrication for Self-Cleaning and Radiative Cooling Effect

Passive radiative cooling (PRC) is a zero-energy-consumption technology that reflects sunlight and radiates heat to cold outer space. In this work, a porous poly(vinylidene fluoride)–poly(methyl methacrylate) (PVDF–PMMA) composite film is fabricated by decorating zinc-imidazolate metal–organic framework (MOF) (ZIF-8) particles obtained by phase inversion. Due to the competent scattering via the coral-like hierarchical structures and the vibration excitations of specific functional groups, the prepared film exhibits good solar reflectance (92.6%) and intermediate infrared emittance (99.1%), with an average sub-ambient cooling of 10.4 °C under a solar radiation intensity of 0.6 AM1.5. Additionally, poly(vinylidene fluoride) has a low surface energy, while the ZIF-8 particles and coral-like hierarchical structures enhance the surface roughness, endowing the surface with significant superhydrophobicity characterized by a water contact angle (WCA) of 157.5° and a sliding angle (SA) of 2°. These films exhibit excellent antibacterial properties. When the content of ZIF-8 particles in the film is 300 mg·L–1, the antibacterial rate reaches 100% after 1 h of treatment. Thus, the ZIF-8 porous poly(vinylidene fluoride)–poly(methyl methacrylate) composite (ZPPP) film has potential application prospects in areas with high health and environmental requirements, such as cold chain transportation and public spaces

Antibacterial PVDF Coral-Like Hierarchical Structure Composite Film Fabrication for Self-Cleaning and Radiative Cooling Effect

Passive radiative cooling (PRC) is a zero-energy-consumption technology that reflects sunlight and radiates heat to cold outer space. In this work, a porous poly(vinylidene fluoride)–poly(methyl methacrylate) (PVDF–PMMA) composite film is fabricated by decorating zinc-imidazolate metal–organic framework (MOF) (ZIF-8) particles obtained by phase inversion. Due to the competent scattering via the coral-like hierarchical structures and the vibration excitations of specific functional groups, the prepared film exhibits good solar reflectance (92.6%) and intermediate infrared emittance (99.1%), with an average sub-ambient cooling of 10.4 °C under a solar radiation intensity of 0.6 AM1.5. Additionally, poly(vinylidene fluoride) has a low surface energy, while the ZIF-8 particles and coral-like hierarchical structures enhance the surface roughness, endowing the surface with significant superhydrophobicity characterized by a water contact angle (WCA) of 157.5° and a sliding angle (SA) of 2°. These films exhibit excellent antibacterial properties. When the content of ZIF-8 particles in the film is 300 mg·L–1, the antibacterial rate reaches 100% after 1 h of treatment. Thus, the ZIF-8 porous poly(vinylidene fluoride)–poly(methyl methacrylate) composite (ZPPP) film has potential application prospects in areas with high health and environmental requirements, such as cold chain transportation and public spaces

Functional Analyse of GLUT1 and GLUT12 in Glucose Uptake in Goat Mammary Gland Epithelial Cells

<div><p>Glucose transport, mediated by glucose transporters, is necessary for mammary gland development and lactation. GLUT1 and GLUT12 could both be expressed in the pregnant and lactating mammary gland to participate in the glucose uptake process. In this study, the goat GLUT1 and GLUT12 genes were cloned from Saanen dairy goats and transfected into goat mammary gland epithelial cells to assess their biological functions and distributions. The results showed that both goat GLUT1 and GLUT12 had 12 predicted membrane-spanning helices. Goat GLUT1 and GLUT12 each influenced the mRNA expression of the other transporter and increased the glucose consumption and lactose yield in GLUT1- and GLUT12-transfected goat mammary gland epithelial cells, respectively. The overexpression of GLUT1 or GLUT12 also increased the expression of amino acid transporters SLC1A5, SLC3A2 and SLC7A5 and affected genes expressions in GMGE cells. Using immunofluorescence staining, GLUT1 was detected throughout the cytoplasm and localized to the Golgi apparatus around the nuclear membrane, whereas GLUT12 was mainly distributed in the perinuclear region and cytoplasm. This study contributes to the understanding of how GLUT1 and GLUT12 cooperate in the incorporation of nutrient uptake into mammary gland epithelial cells and the promotion of milk synthesis in the goat mammary gland during lactation.</p></div

Supplementary document for Broadband ASE source-enabled self-homodyne DA-RoF fronthaul using cascaded SOAs and multicore fiber - 6853676.pdf

Supplemental Documen

The effects of different OGD durations on MTT reduction and apoptosis and necrosis in astrocytes.

<p>Cells were exposed to OGD for 4, 6 or 8 h, followed by reperfusion for 0, 24 or 48 h. The percentage of MTT reduction, apoptosis and necrosis was assayed after various durations of reperfusion. Values are expressed as percentage of control values and are from 3 to 6 independent experiments. * <i>P</i><0.05; ** <i>P</i><0.01; *** <i>P</i><0.001, compared with control group.</p