Additional file 1 of High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China

Supplementary material 1

MOESM1 of Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8

Additional file 1: Table S1. Degradation capability of strain CNP-8 for various nitrophenols. Figure S1. HPLC identification of the intermediates of 2C4NP degradation by strain CNP-8. Figure S2. Mass spectra of the acetylated derivatives of the intermediates during 2C4NP degradation by strain CNP-8. (A) Acetylated derivative of authentic CHQ. (B) Acetylated metabolite I. (C) Acetylated derivative of authentic BT. (D) Acetylated metabolite II. Figure S3. Transformation of 2C4NP (A) and BT (B) by the cell extract of 2C4NP-induced strain CNP-8

Carbon Dioxide Microbubble Bursting Ionization Mass Spectrometry

Aerosols generated by bubble bursting have been proved to promote the extraction of analytes and have ultrahigh electric fields at their water–air interfaces. This study presented a simple and efficient ionization method, carbon dioxide microbubble bursting ionization (CDMBI), without the presence of an exogenous electric field (namely, zero voltage), by simulating the interfacial chemistries of sea spray aerosols. In CDMBI, microbubbles are generated in situ by continuous input of carbon dioxide into an aqueous solution containing low-concentration analytes. The microbubbles extract low- and high-polarity analytes as they pass through the aqueous solution. Upon reaching the water–air interface, these microbubbles burst to produce charged aerosol microdroplets with an average diameter of 260 μm (8.1–10.4 nL in volume), which are immediately transferred to a mass spectrometer for the detection and identification of extracted analytes. The above analytical process occurs every 4.2 s with a stable total ion chromatogram (relative standard deviation: 9.4%) recorded. CDMBI mass spectrometry (CDMBI-MS) can detect surface-active organic compounds in aerosol microdroplets, such as perfluorooctanoic acid, free fatty acids epoxidized by bubble bursting, sterols, and lecithins in soybean and egg, with the limit of detection reaching the level of fg/mL. In addition, coupling CDMBI-MS with an exogenous voltage yields relatively weak gains in ionization efficiency and sensitivity of analysis. The results suggested that CDMBI can simultaneously accomplish both bubbling extraction and microbubble bursting ionization. The mechanism of CDMBI involves bubbling extraction, proton transfer, inlet ionization, and electrospray-like ionization. Overall, CDMBI-MS can work in both positive and negative ion modes without necessarily needing an exogenous high electric field for ionization and quickly detect trace surface-active analytes in aqueous solutions

Flow diagram showing the selection process of articles.

*Consider, if feasible to do so, reporting the number of records identified from each database or register searched (rather than the total number across all databases/registers). **If automation tools were used, indicate how many records were excluded by a human and how many were excluded by automation tools. From: Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, et al. The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ 2021;372:n71. doi: 10.1136/bmj.n71 For more information, visit: http://www.prisma-statement.org/.</p

General information of the included studies.

IntroductionLung cancer is the primary cause of cancer-related deaths worldwide, with high rates of morbidity and mortality. The most effective treatment for early stage (I-II) non-small cell lung cancer (NSCLC) is surgical resection. However, the extent of mediastinal lymph nodes removal required and the impact of their removal remains controversial. This systematic review and meta-analysis aimed to evaluate the postoperative complications in patients with stage I-II NSCLC who received mediastinal lymph node dissection (MLND) or mediastinal lymph node sampling (MLNS).Methods and analysisAccording to the predefined inclusion criteria, we will conduct a comprehensive search for randomized controlled trials (RCTs) and observational studies examining the postoperative complications of MLND compared to MLNS in patients with stage I-II NSCLC. The search will be performed across multiple databases including PubMed, Embase, the Cochrane Library, CNKI, WanFang, Sinomed, VIP, Duxiu, and Web of Science from inception to February 2024. Additionally, relevant literature references will be retrieved and hand searching of pertinent journals will be conducted. Screening, data extraction, and quality assessment will be performed by two independent reviewers. Review Manager 5.4 will be applied in analyzing and synthesizing. The Grading of Recommendations Assessment, Development and Evaluation (GRADE) will be used to assess the quality of evidence for the whole RCTs and used Newcastle-Ottawa scale to assess the methodologic quality of observational studies.Ethics and disseminationThis study did not include personal information. Ethical approval was not required for this study. This study is based on a secondary analysis of the literature, so ethical review approval is not required. The final report will be published in a peer-reviewed journal.ConclusionThis systematic review will contribute to compare the safety and survival benefits of these two surgical techniques for the treatment of early stage NSCLC, to further guide the selection of surgical approaches.Trial registrationThe protocol of the systematic review has been registered on Open Science Framework, with a registration number of DOI https://doi.org/10.17605/OSF.IO/N2Y5D.</div

Analysis of cDNA and the protein sequence of porcine 4Ig-B7-H3.

<p>A, The cloned cDNA. The boxed, underlined and gray colored sequences show the positions of all used primers. The numbers in the brackets are the exon order of porcine B7-H3 in the genome. The strong ATG and TGA are initiation and stop codons. All exons contain the complete sequence in the genome but exon 1 in the cDNA. The numbers on the left are the base count of cDNA. B, The predicted protein sequence. The numbers on the left are the count of amino acids . All predicted domains are marked above the sequence. C, Alignment of the duplicated amino acid sequences of the two IgV and IgC-like domains, respectively. Asterisks indicate the identical amino acids. D, Schematic structure of CD276 nucleotide sequence in the porcine genome. The filled panes and lines represent Exons and Introns, respectively. The numbers under the schematics is the order of the Exons in the genome.</p

PRISMA-P-checklist.

IntroductionLung cancer is the primary cause of cancer-related deaths worldwide, with high rates of morbidity and mortality. The most effective treatment for early stage (I-II) non-small cell lung cancer (NSCLC) is surgical resection. However, the extent of mediastinal lymph nodes removal required and the impact of their removal remains controversial. This systematic review and meta-analysis aimed to evaluate the postoperative complications in patients with stage I-II NSCLC who received mediastinal lymph node dissection (MLND) or mediastinal lymph node sampling (MLNS).Methods and analysisAccording to the predefined inclusion criteria, we will conduct a comprehensive search for randomized controlled trials (RCTs) and observational studies examining the postoperative complications of MLND compared to MLNS in patients with stage I-II NSCLC. The search will be performed across multiple databases including PubMed, Embase, the Cochrane Library, CNKI, WanFang, Sinomed, VIP, Duxiu, and Web of Science from inception to February 2024. Additionally, relevant literature references will be retrieved and hand searching of pertinent journals will be conducted. Screening, data extraction, and quality assessment will be performed by two independent reviewers. Review Manager 5.4 will be applied in analyzing and synthesizing. The Grading of Recommendations Assessment, Development and Evaluation (GRADE) will be used to assess the quality of evidence for the whole RCTs and used Newcastle-Ottawa scale to assess the methodologic quality of observational studies.Ethics and disseminationThis study did not include personal information. Ethical approval was not required for this study. This study is based on a secondary analysis of the literature, so ethical review approval is not required. The final report will be published in a peer-reviewed journal.ConclusionThis systematic review will contribute to compare the safety and survival benefits of these two surgical techniques for the treatment of early stage NSCLC, to further guide the selection of surgical approaches.Trial registrationThe protocol of the systematic review has been registered on Open Science Framework, with a registration number of DOI https://doi.org/10.17605/OSF.IO/N2Y5D.</div

Analysis of a putative receptor of porcine 4Ig-B7-H3.

<p>A, Binding of porcine 4Ig-B7-H3-Fc to T cells. T cells treated in different conditions were stained with a combination of anti-CD3 mAb with Fc (solid line histogram) or isotypic Ab (dotted line histogram) or 4Ig-B7-H3-Fc (filled histogram). Left plot: T cells in PBMC were not activated. Middle plot: T cells in primary PBMCs were stimulated with 5 µg/ml of PHA for 24 h. Right plot: T cells in primary PBMCs were stimulated with 1 µg/ml of anti-porcine CD3 mAb coated on the 96-well plate for 48 h. B, PD-1 gene was transfected into 293 T cells. PD-1 expressed on the 293 T cells was stained with anti-PD-1 mAb (left, filled histogram) or 4Ig-B7-H3-Fc protein (right, filled histogram) or Fc (solid line histogram) or isotypic Ab (dotted line histogram) 24 hours later.</p

Distribution and analysis of the porcine B7-H3.

<p>A, Dot-blot hybridization assay of mRNA from different tissues using the probes of 4Ig-B7-H3. Samples of PBMCs, PAM, heart, liver, spleen, lung, kidney, brain, lymph nodes, small intestine, tonsil were loaded from dot 1 to 11, respectively, with positive control (dot 12) and negative control (dot 13). The upper and lower panels were B7-H3 and β-actin, respectively. B, Analysis of the porcine B7-H3 isoforms. The normalized PCR products of PBMCs, PAM, heart, liver, spleen, lung, kidney, brain, lymph nodes, small intestine, tonsil were loaded from lane 2 to 12 with DNA ladder marker (lane 1). The upper and lower panels were the PCR products of B7-H3 and β-actin, respectively.</p

Analysis of porcine B7-H3 expression on monocytes.

<p>A, Immunoprecipitation assay. The membrane protein extracted from the porcine 4Ig-B7-H3-transfected 293 T cells (Lane 1) or vacant plasmid-transfected 293 T cells (Lane 2) was pulled down with anti-porcine B7-H3 mAb-bound protein-G agarose. The pulled-down proteins were submitted to western-blot assay. Lane 3: anti-porcine B7-H3 mAb-bound protein-G agarose control. Lane 4: protein marker. B, The analysis of the porcine 2Ig-(left plot) and 4Ig-B7-H3 (right plot) expressed on 293 T cells stained by the anti-porcine B7-H3 mAb (filled histogram) or isotypic Ab (open histogram). C, non-activated monocytes were stained with a combination of anti-CD14 with either anti-B7-H3 mAb (filled histogram) or isotypic Ab (open histogram). D, The monocytes were co-cultured with IFN-γ in a final concentration of 30 ng/ml (left plots) or LPS at a final concentration of 1 µg/ml (right plots) for 24 h (upper plots) or 48 h plots (lower plots). On the indicated time points, the monocytes were harvested to be stained with a combination of anti-CD14 with either anti-B7-H3 mAb (filled histogram) or isotypic Ab (open histogram).</p