Single-Particle Tracking Reveals Anti-Persistent Subdiffusion in Cell Extracts

Single-particle tracking (SPT) has become a powerful tool to quantify transport phenomena in complex media with unprecedented detail. Based on the reconstruction of individual trajectories, a wealth of informative measures become available for each particle, allowing for a detailed comparison with theoretical predictions. While SPT has been used frequently to explore diffusive transport in artificial fluids and inside living cells, intermediate systems, i.e., biochemically active cell extracts, have been studied only sparsely. Extracts derived from the eggs of the clawfrog Xenopus laevis, for example, are known for their ability to support and mimic vital processes of cells, emphasizing the need to explore also the transport phenomena of nano-sized particles in such extracts. Here, we have performed extensive SPT on beads with 20 nm radius in native and chemically treated Xenopus extracts. By analyzing a variety of distinct measures, we show that these beads feature an anti-persistent subdiffusion that is consistent with fractional Brownian motion. Chemical treatments did not grossly alter this finding, suggesting that the high degree of macromolecular crowding in Xenopus extracts equips the fluid with a viscoelastic modulus, hence enforcing particles to perform random walks with a significant anti-persistent memory kernel

Feasibility of low-dose digital pulsed video-fluoroscopic swallow exams (VFSE): effects on radiation dose and image quality

Background: Fluoroscopy is a frequently used examination in clinical routine without appropriate research evaluation latest hardware and software equipment. Purpose: To evaluate the feasibility of low-dose pulsed video-fluoroscopic swallowing exams (pVFSE) to reduce dose exposure in patients with swallowing disorders compared to high-resolution radiograph examinations (hrVFSE) serving as standard of reference. Material and Methods: A phantom study (Alderson-Rando Phantom, 60 thermoluminescent dosimeters [TLD]) was performed for dose measurements. Acquisition parameters were as follows: (i) pVFSE: 76.7 kV, 57 mA, 0.9 Cu mm, pulse rate/s 30;(ii) hrVFSE: 68.0 kV, 362 mA, 0.2 Cu mm, pictures 30/s. The dose area product (DAP) indicated by the detector system and the radiation dose derived from the TLD measurements were analyzed. In a patient study, image quality was assessed qualitatively (5-point Likert scale, 5 = hrVFSE;two independent readers) and quantitatively (SNR) in 35 patients who subsequently underwent contrast-enhanced pVFSE and hrVFSE. Results: Phantom measurements showed a dose reduction per picture of factor 25 for pVFSE versus hrVFSE images (0.0025 mGy versus 0.062 mGy). The DAP (mu Gym 2) was 28.0 versus 810.5 (pVFSE versus hrVFSE) for an average examination time of 30 s. Direct and scattered organ doses were significantly lower for pVFSE as compared to hrVFSE (P< 0.05). Image quality was rated 3.9 +/- 0.5 for pVFSE versus the hrVFSE standard;depiction of the contrast agent 4.8 +/- 0.3;noise 3.6 +/- 0.5 (P< 0.05);SNR calculations revealed a relative decreased of 43.9% for pVFSE as compared to hrVFSE. Conclusion: Pulsed VFSE is feasible, providing diagnostic image quality at a significant dose reduction as compared to hrVFSE

Condensation of Ideal Bose Gas Confined in a Box Within a Canonical Ensemble

We set up recursion relations for the partition function and the ground-state occupancy for a fixed number of non-interacting bosons confined in a square box potential and determine the temperature dependence of the specific heat and the particle number in the ground state. A proper semiclassical treatment is set up which yields the correct small-T-behavior in contrast to an earlier theory in Feynman's textbook on Statistical Mechanics, in which the special role of the ground state was ignored. The results are compared with an exact quantum mechanical treatment. Furthermore, we derive the finite-size effect of the system.Comment: 18 pages, 8 figure

HAT-P-11: Discovery of a Second Planet and a Clue to Understanding Exoplanet Obliquities

HAT-P-11 is a mid-K dwarf that hosts one of the first Neptune-sized planets found outside the solar system. The orbit of HAT-P-11b is misaligned with the star's spin --- one of the few known cases of a misaligned planet orbiting a star less massive than the Sun. We find an additional planet in the system based on a decade of precision radial velocity (RV) measurements from Keck/HIRES. HAT-P-11c is similar to Jupiter in its mass ($M_P \sin{i} = 1.6\pm0.1$ $M_J$) and orbital period ($P = 9.3^{+1.0}_{-0.5}$ year), but has a much more eccentric orbit ($e=0.60\pm0.03$). In our joint modeling of RV and stellar activity, we found an activity-induced RV signal of $\sim$7 m s$^{-1}$, consistent with other active K dwarfs, but significantly smaller than the 31 m s$^{-1}$ reflex motion due to HAT-P-11c. We investigated the dynamical coupling between HAT-P-11b and c as a possible explanation for HAT-P-11b's misaligned orbit, finding that planet-planet Kozai interactions cannot tilt planet b's orbit due to general relativistic precession; however, nodal precession operating on million year timescales is a viable mechanism to explain HAT-P-11b's high obliquity. This leaves open the question of why HAT-P-11c may have such a tilted orbit. At a distance of 38 pc, the HAT-P-11 system offers rich opportunities for further exoplanet characterization through astrometry and direct imaging.Comment: 16 pages, 11 figures, 4 tables. Accepted to A

Observing the LMC with APEX: Signatures of Large-scale Feedback in the Molecular Clouds of 30 Doradus

Stellar feedback plays a crucial role in star formation and the life cycle of molecular clouds. The intense star formation region 30 Doradus, which is located in the Large Magellanic Cloud (LMC), is a unique target for detailed investigation of stellar feedback owing to the proximity of the hosting galaxy and modern observational capabilities that together allow us to resolve individual molecular clouds $-$ nurseries of star formation. We study the impact of large-scale feedback on the molecular gas using the new observational data in the $^{12}$CO(3$-$2) line obtained with the APEX telescope. Our data cover an unprecedented area of 13.8 sq. deg. of the LMC disc with a spatial resolution of 5 pc and provide an unbiased view of the molecular clouds in the galaxy. Using this data, we located molecular clouds in the disc of the galaxy, estimated their properties, such as the areal number density, relative velocity and separation, width of the line profile, CO line luminosity, size, and virial mass, and compared these properties of the clouds of 30 Doradus with those in the rest of the LMC disc. We find that, compared with the rest of the observed molecular clouds in the LMC disc, those in 30 Doradus show the highest areal number density; they are spatially more clustered, they move faster with respect to each other, and they feature larger linewidths. In parallel, we do not find statistically significant differences in such properties as the CO line luminosity, size, and virial mass between the clouds of 30 Doradus and the rest of the observed field. We interpret our results as signatures of gas dispersal and fragmentation due to high-energy large-scale feedback.Comment: Accepted for publication in A&A. 13 pages, 7 figures, 4 table

K2-19b and c are in a 3:2 Commensurability but out of Resonance: A Challenge to Planet Assembly by Convergent Migration

K2-19b and c were among the first planets discovered by NASA's K2 mission and together stand in stark contrast with the physical and orbital properties of the solar system planets. The planets are between the size of Uranus and Saturn at 7.0 ± 0.2 R⊕ and 4.1 ± 0.2 R⊕, respectively, and reside a mere 0.1% outside the nominal 3:2 mean-motion resonance. They represent a different outcome of the planet formation process than the solar system, as well as the vast majority of known exoplanets. We measured the physical and orbital properties of these planets using photometry from K2, Spitzer, and ground-based telescopes, along with radial velocities from Keck/HIRES. Through a joint photodynamical model, we found that the planets have moderate eccentricities of e ≈ 0.20 and well-aligned apsides Δϖ ≈ 0°. The planets occupy a strictly nonresonant configuration: the resonant angles circulate rather than librate. This defies the predictions of standard formation pathways that invoke convergent or divergent migration, both of which predict Δ ≈ 180° and eccentricities of a few percent or less. We measured masses of M_(p,b) = 32.4 ± 1.7 M⊕ and M_(p,c) = 10.8 ± 0.6 M⊕. Our measurements, with 5% fractional uncertainties, are among the most precise of any sub-Jovian exoplanet. Mass and size reflect a planet's core/envelope structure. Despite having a relatively massive core of M_(core) ≈ 15 M⊕, K2-19b is envelope-rich, with an envelope mass fraction of roughly 50%. This planet poses a challenge to standard models of core-nucleated accretion, which predict that cores ≳10 M⊕ will quickly accrete gas and trigger runaway accretion when the envelope mass exceeds that of the core

The impact of concomitant chronic total occlusion on clinical outcomes in patients undergoing transcatheter aortic valve replacement: a large single-center analysis

BackgroundCoronary artery disease (CAD) is a common finding in patients with severe aortic stenosis undergoing transcatheter aortic valve replacement (TAVR). However, the impact on prognosis of chronic total occlusions (CTOs), a drastic expression of CAD, remains unclear.Methods and resultsWe retrospectively reviewed 1,487 consecutive TAVR cases performed at a single tertiary care medical center. Pre-TAVR angiograms were analyzed for the presence of a CTO. At the time of TAVR, 11.2% (n = 167) patients had a CTO. There was no significant association between the presence of a CTO and in-hospital or 30-day mortality. There was also no difference in long-term survival. LV ejection fraction and mean aortic gradients were lower in the CTO group.ConclusionsOur analysis suggests that concomitant CTO lesions in patients undergoing TAVR differ in their risk profile and clinical findings to patients without CTO. CTO lesion per se were not associated with increased mortality, nevertheless CTOs which supply non-viable myocardium in TAVR population were associated with increased risk of death. Additional research is needed to evaluate the prognostic significance of CTO lesions in TAVR patients