Variation detection based on next-generation sequencing of type Chinese 1 strains of Toxoplasma gondii with different virulence from China

A: Summary of annotation for SNPs; B: Summary of annotation for indels; C: Summary of annotation for SVs; D: Summary of annotation for CNVs. (DOCX 18 kb

Synthesis of Glaucogenin D, a Structurally Unique Disecopregnane Steroid with Potential Antiviral Activity

The first chemical synthesis of glaucogenin D, a 13,14:14,15-disecopregnane steroid with potential antiviral activity, has been accomplished in 12 steps from a hirundigenin-type intermediate. The present route would also be amenable to the synthesis of natural and unnatural glaucogenin derivatives for SAR studies

Scalable Synthesis of Cyclocitrinol

A 10-step synthesis of the C25 steroid natural product cyclocitrinol from inexpensive, commercially available pregnenolone is reported. This synthesis features a biomimetic cascade rearrangement to efficiently construct the challenging bicyclo[4.4.1] A/B ring system, which enabled a gram-scale synthesis of the bicyclo[4.4.1] enone intermediate <b>18</b> in only nine steps. This work also provides experimental support for the biosynthetic origin of cyclocitrinol

A Comprehensive and Effective Mass Spectrometry-Based Screening Strategy for Discovery and Identification of New Brassinosteroids from Rice Tissues

The exploration and identification of new brassinosteroid (BR) compounds is critical to improve the biosynthetic research of BRs and expand the chemodiversity of active BRs. However, traditional methods are labor-intensive, time-consuming, and less sensitive. Here, we present a facile screening strategy for discovering and identifying novel BRs from plant tissues based on ultra performance liquid chromatography-mass spectrometry (UPLC-MS). A total of 14 potential BRs were discovered from only 1 g of rice tissues and structurally elucidated by following a MS-based clue, acquired through multiple reaction monitoring (MRM) data-dependent enhanced product ion (EPI) scan, high resolution MS, and MS survey-dependent MS/MS. One of the 14 candidates was identified as 6-deoxo-28-homotyphasterol, a brand new BR compound that is reported for the first time in the BRs biosynthesis pathway. Detailed comparison with reference standards and quantitative level analysis in rice BRs mutants confirmed the availability of the other candidates. This effective, yet simple method provides an efficient way to find more and more chemically new BR biosynthetic intermediates in plants, which is significant for complementing the biosynthesis and metabolism network of BRs. This strategy may also be used to discover unknown compounds of other plant hormone species as well as their key metabolites

Study on the Mechanism of Ionic Stabilizers on Shale Gas Reservoir Mechanics in Northwestern Hunan

The shale of the lower Cambrian Niutitang formation in northwestern Hunan is an ideal reservoir for shale gas. There is a close connection between borehole stability and drilling fluid in shale gas drilling. Ionic stabilizer is a new type of stratum consolidation agent that inhibits the hydration expansion of clay minerals and improves mechanical strength of the borehole. The traditional idea of pore wall protection is to use drilling fluid additives to prevent shale from interacting with water. However, ionic stabilizer can change the hydrophilic of clay minerals in shale, making the particles become hydrophobic and dense, therefore, the formation stability can be enhanced simultaneously. The material used in this paper is different from the normal ionic stabilizer, some chemical bonds that have been changed in the new material called enhanced normality ionic (ENI) stabilizer. This paper utilized the shale samples those obtained from Niutitang formation to study the connection between ENI and the mechanical properties of shale. Mechanical tests and microscopic pore tests were performed on different samples which were soaked in water and the ENI with different concentrations. It has been found through tests that ENI can inhibit the development of shale pores, and as the concentration increases, the inhibition increases. In addition, as the ENI concentration increases, the uniaxial compressive strength and Young&rsquo;s modulus of the shale increase, and the ratio of stability coefficients decreases. It can be concluded that the ENI can improve the mechanical strength of carbon shale, and prevent the development of rock damage. Moreover, it can improve the ability of rock to resist damage, and enhance borehole stability initiatively

Biosynthesis Investigations of Terpenoid, Alkaloid, and Flavonoid Antimicrobial Agents Derived from Medicinal Plants

The overuse of antibiotics in the past decades has led to the emergence of a large number of drug-resistant microorganisms. In recent years, the infection rate caused by multidrug-resistant microorganisms has been increasing, which has become one of the most challenging problems in modern medicine. Plant-derived secondary metabolites and their derivatives have been identified to display significant antimicrobial abilities with good tolerance and less adverse side effects, potentially having different action mechanisms with antibiotics of microbial origin. Thus, these phyto-antimicrobials have a good prospect in the treatment of multidrug-resistant microorganisms. Terpenoids, alkaloids, and flavonoids made up the predominant part of the currently reported phytochemicals with antimicrobial activities. Synthetic biology research around these compounds is one of the hotspot fields in recent years, which not only has illuminated the biosynthesis pathways of these phyto-antimicrobials but has also offered new methods for their production. In this review, we discuss the biosynthesis investigations of terpenoid, alkaloid, and flavonoid antimicrobial agents—using artemisinin and oleanolic acid (terpenoids), berberine and colchicine (alkaloids), and baicalin (flavonoids) as examples—around their antimicrobial action mechanisms, biosynthesis pathway elucidation, key enzyme identification, and heterologous production, in order to provide useful hints for plant-derived antimicrobial agent discovery and development

The Protective Effects of 5-Methoxytryptamine-α-lipoic Acid on Ionizing Radiation-Induced Hematopoietic Injury

Antioxidants are prospective radioprotectors because of their ability to scavenge radiation-induced reactive oxygen species (ROS). The hematopoietic system is widely studied in radiation research because of its high radiosensitivity. In the present study, we describe the beneficial effects of 5-methoxytryptamine-α-lipoic acid (MLA), which was synthesized from melatonin and α-lipoic acid, against radiation-induced hematopoietic injury. MLA administration significantly enhanced the survival rate of mice after 7.2 Gy total body irradiation. The results showed that MLA not only markedly increased the numbers and clonogenic potential of hematopoietic cells but also decreased DNA damage, as determined by flow cytometric analysis of histone H2AX phosphorylation. In addition, MLA decreased the levels of ROS in hematopoietic cells by inhibiting NOX4 expression. These data demonstrate that MLA prevents radiation-induced hematopoietic syndrome by increasing the number and function of and by inhibiting DNA damage and ROS production in hematopoietic cells. These data suggest MLA is beneficial for the protection of radiation injuries