Evolution and diversity of secretome genes in the apicomplexan parasite Theileria annulata

<b>BACKGROUND</b>: Little is known about how apicomplexan parasites have evolved to infect different host species and cell types. Theileria annulata and Theileria parva invade and transform bovine leukocytes but each species favours a different host cell lineage. Parasite-encoded proteins secreted from the intracellular macroschizont stage within the leukocyte represent a critical interface between host and pathogen systems. Genome sequencing has revealed that several Theileria-specific gene families encoding secreted proteins are positively selected at the inter-species level, indicating diversification between the species. We extend this analysis to the intra-species level, focusing on allelic diversity of two major secretome families. These families represent a well-characterised group of genes implicated in control of the host cell phenotype and a gene family of unknown function. To gain further insight into their evolution and function, this study investigates whether representative genes of these two families are diversifying or constrained within the T. annulata population. <b>RESULTS</b>: Strong evidence is provided that the sub-telomerically encoded SVSP family and the host-nucleus targeted TashAT family have evolved under contrasting pressures within natural T. annulata populations. SVSP genes were found to possess atypical codon usage and be evolving neutrally, with high levels of nucleotide substitutions and multiple indels. No evidence of geographical sub-structuring of allelic sequences was found. In contrast, TashAT family genes, implicated in control of host cell gene expression, are strongly conserved at the protein level and geographically sub-structured allelic sequences were identified among Tunisian and Turkish isolates. Although different copy numbers of DNA binding motifs were identified in alleles of TashAT proteins, motif periodicity was strongly maintained, implying conserved functional activity of these sites. <b>CONCLUSIONS</b>: This analysis provides evidence that two distinct secretome genes families have evolved under contrasting selective pressures. The data supports current hypotheses regarding the biological role of TashAT family proteins in the management of host cell phenotype that may have evolved to allow adaptation of T. annulata to a specific host cell lineage. We provide new evidence of extensive allelic diversity in representative members of the enigmatic SVSP gene family, which supports a putative role for the encoded products in subversion of the host immune response

Tabulation and summary of thermodynamic effects data for developed cavitation on ogive-nosed bodies

Thermodynamic effects data for developed cavitation on zero and quarter caliber ogives in Freon 113 and water are tabulated and summarized. These data include temperature depression (delta T), flow coefficient (C sub Q), and various geometrical characteristics of the cavity. For the delta T tests, the free-stream temperature varied from 35 C to 95 C in Freon 113 and from 60 C to 125 C in water for a velocity range of 19.5 m/sec to 36.6 m/sec. Two correlations of the delta T data by the entrainment method are presented. These correlations involve different combinations of the Nusselt, Reynolds, Froude, Weber, and Peclet numbers and dimensionless cavity length

Correlations by the entrainment theory of thermodynamic effects for developed cavitation in venturis and comparisons with ogive data

A semi-empirical entrainment theory was employed to correlate the measured temperature depression, Delta T, in a developed cavity for a venturi. This theory correlates Delta t in terms of the dimensionless numbers of Nusselt, Reynolds, Froude, Weber and Peclet, and dimensionless cavity length, L/D. These correlations are then compared with similar correlations for zero and quarter caliber ogives. In addition, cavitation number data for both limited and developed cavitation in venturis are presented

Diversity in parasitic nematode genomes: the microRNAs of Brugia pahangi and Haemonchus contortus are largely novel

<b>BACKGROUND:</b> MicroRNAs (miRNAs) play key roles in regulating post-transcriptional gene expression and are essential for development in the free-living nematode Caenorhabditis elegans and in higher organisms. Whether microRNAs are involved in regulating developmental programs of parasitic nematodes is currently unknown. Here we describe the the miRNA repertoire of two important parasitic nematodes as an essential first step in addressing this question. <b>RESULTS:</b> The small RNAs from larval and adult stages of two parasitic species, Brugia pahangi and Haemonchus contortus, were identified using deep-sequencing and bioinformatic approaches. Comparative analysis to known miRNA sequences reveals that the majority of these miRNAs are novel. Some novel miRNAs are abundantly expressed and display developmental regulation, suggesting important functional roles. Despite the lack of conservation in the miRNA repertoire, genomic positioning of certain miRNAs within or close to specific coding genes is remarkably conserved across diverse species, indicating selection for these associations. Endogenous small-interfering RNAs and Piwi-interacting (pi)RNAs, which regulate gene and transposon expression, were also identified. piRNAs are expressed in adult stage H. contortus, supporting a conserved role in germline maintenance in some parasitic nematodes. <b>CONCLUSIONS:</b> This in-depth comparative analysis of nematode miRNAs reveals the high level of divergence across species and identifies novel sequences potentially involved in development. Expression of novel miRNAs may reflect adaptations to different environments and lifestyles. Our findings provide a detailed foundation for further study of the evolution and function of miRNAs within nematodes and for identifying potential targets for intervention

Growth regulator trials. Part 1

This summary covers 1981 trials on chlormequat plant growth regulant (formulated as Cycocel 750A or Halloween), ECO organic seed dressing and a range of organic foliar sprays. Rates and times of application of chlormequat on wheat. 81A22, 81BA3, 81Cl8, 81E25, 81M22, 81MT2, 81N4, 81SG36, 81WH4. Effects of chlormequat on wheat varieties (S4 trials). 81A6, 81BA7, 81C6, 81E6, 81M9, 81M13, 81MT9, 81N10, 81SG26, 81WH19. Trials comparing organic seed treatments, organic foliar sprays and plant growth regulators. 81WH61, 81LG34, 81LG48, 81ME27a, 81ME276, 81TS39, 81ES51, 81ES52, 81ES53, 81ES54, 81NA57, 81N4A, 81MT2A, 81TS38A. H. McCashney, Cadoux Un-numbered, Newdegate Research Station

Experimental summaries.

Wheat Yield Potential Trials, 81BA4, 81WH7, 81KAS.Wheat-Regenerating Sandplain Lupin Rotation Trials, 79GE38, 79GE42.Nitrogen x Cycocel x Foliar Nutrients x Fungicide-on Wheat,81KA6. Historical Wheat Trials, 81A3, 81C4, 81SG22, 81WH6. Time of Planting on Cereal Yields, 81NA8, 81KA55

Fluxoid formation: size effects and non-equilibrium universality

Simple causal arguments put forward by Kibble and Zurek suggest that the scaling behaviour of condensed matter at continuous transitions is related to the familiar universality classes of the systems at quasi-equilibrium. Although proposed 25 years ago or more, it is only in the last few years that it has been possible to devise experiments from which scaling exponents can be determined and in which this scenario can be tested. In previous work, an unusually high Kibble-Zurek scaling exponent was reported for spontaneous fluxoid production in a single isolated superconducting Nb loop, albeit with low density. Using analytic approximations backed up by Langevin simulations, we argue that densities as small as these are too low to be attributable to scaling, and are conditioned by the small size of the loop. We also reflect on the physical differences between slow quenches and small rings, and derive some criteria for these differences, noting that recent work on slow quenches does not adequately explain the anomalous behaviour seen here.Comment: 7 pages, 4 figures, presentation given at CMMP 201

Defect formation in superconducting rings: external fields and finite-size effects

Consistent with the predictions of Kibble and Zurek, scaling behaviour has been seen in the production of fluxoids during temperature quenches of superconducting rings. However, deviations from the canonical behaviour arise because of finite-size effects and stray external fields. Technical developments, including laser heating and the use of long Josephson tunnel junctions, have improved the quality of data that can be obtained. With new experiments in mind we perform large-scale 3D simulations of quenches of small, thin rings of various geometries with fully dynamical electromagnetic fields, at nonzero externally applied magnetic flux. We find that the outcomes are, in practice, indistinguishable from those of much simpler Gaussian analytical approximations in which the rings are treated as one-dimensional systems and the magnetic field fluctuation-free.Comment: 10 pages, 3 figures, presentation at QFS2012, to appear in JLT