Transient increase of ATP as a response to temperature up-shift in Escherichia coli

BACKGROUND: Escherichia coli induces the heat shock response to a temperature up-shift which is connected to the synthesis of a characteristic set of proteins, including ATP dependent chaperones and proteases. Therefore the balance of the nucleotide pool is important for the adaptation and continuous function of the cell. Whereas it has been observed in eukaryotic cells, that the ATP level immediately decreased after the temperature shift, no data are available for E. coli about the adenosine nucleotide levels during the narrow time range of minutes after a temperature up-shift. RESULTS: The current study shows that a temperature up-shift is followed by a very fast significant transient increase of the cellular ATP concentration within the first minutes. This increase is connected to a longer lasting elevation of the cellular respiration and glucose uptake. Also the mRNA level of typical heat shock genes increases within only one minute after the heat-shock. CONCLUSION: The presented data prove the very fast response of E. coli to a heat-shock and that the initial response includes the increase of the ATP pool which is important to fulfil the need of the cell for new syntheses, as well as for the function of chaperones and proteases

Dynamics of the Energy Charge and sum of adenosine phosphates (AXP) during a cultivation of W3110 with temperature up-shift from 30 to 42°C

<p><b>Copyright information:</b></p><p>Taken from "Transient increase of ATP as a response to temperature up-shift in "</p><p>Microbial Cell Factories 2005;4():9-9.</p><p>Published online 1 Apr 2005</p><p>PMCID:PMC1087501.</p><p>Copyright © 2005 Soini et al; licensee BioMed Central Ltd.</p> Data were calculated from primary concentrations of the adenosine nucleotides shown in Figure 2

Response of the adenosine nucleotide pool to a temperature up-shift from 30 to 42°C in W3110

<p><b>Copyright information:</b></p><p>Taken from "Transient increase of ATP as a response to temperature up-shift in "</p><p>Microbial Cell Factories 2005;4():9-9.</p><p>Published online 1 Apr 2005</p><p>PMCID:PMC1087501.</p><p>Copyright © 2005 Soini et al; licensee BioMed Central Ltd.</p> (A) ATP and cultivation temperature, (B) ADP, (C) AMP. The data were obtained during the batch cultivation shown in Figure 1

Batch cultivation of W3110 with a temperature up-shift performed at time 0 by switching the temperature set-point from 30 to 42°C

<p><b>Copyright information:</b></p><p>Taken from "Transient increase of ATP as a response to temperature up-shift in "</p><p>Microbial Cell Factories 2005;4():9-9.</p><p>Published online 1 Apr 2005</p><p>PMCID:PMC1087501.</p><p>Copyright © 2005 Soini et al; licensee BioMed Central Ltd.</p> (A) OD(○), temperature (—); (B) glucose (□), acetate (△); (C) q(○), q(▽), RQ (□), (D) pH (△), relative units of ammonia added (+). The grey area indicates the time period of about 5 min during which the temperature increased

Response of mRNAs in a stirred-flask cultivation of W3110 with a temperature shock from 30°C to 42°C

<p><b>Copyright information:</b></p><p>Taken from "Transient increase of ATP as a response to temperature up-shift in "</p><p>Microbial Cell Factories 2005;4():9-9.</p><p>Published online 1 Apr 2005</p><p>PMCID:PMC1087501.</p><p>Copyright © 2005 Soini et al; licensee BioMed Central Ltd.</p> For experimental details see Materials and Methods